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The Expression of Zinc Transporters Changed in the Intestine of Weaned Pigs Exposed to Zinc Chitosan Chelate

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Abstract

This study was conducted to investigate the effect of zinc chitosan chelate (CS-Zn) on zinc transporter expression and content of tissue zinc in weaned piglets. A total of 90 weaned pigs (Duroc × Landrace × Yorkshire) were randomly allocated to treatment groups with supplementation of 100 mg/kg zinc as ZnSO4, 100 mg/kg zinc as mixture of ZnSO4 and chitosan, or 100 mg/kg zinc as CS-Zn, respectively. After 30 days of trial, 18 piglets (six pigs per treatment) were killed and the samples of duodenal mucosa were taken for analysis of zinc transporter mRNA expressions and protein abundance. The results show that CS-Zn more effectively increases (p < 0.05) the average daily gain (ADG) and serum zinc concentration. Zinc concentration in the liver and kidney did not differ between treatments. The mRNA expressions of ZnT1, ZIP4, and ZIP5 in CS-Zn treatment were all upregulated (p < 0.05) than ZnSO4 or mixture of ZnSO4 and chitosan groups. ZnT1 abundance was greater (p < 0.05) with CS-Zn as compared with ZnSO4 and mixture of ZnSO4 and chitosan treatments, whereas ZIP4 and ZIP5 abundance was higher (p < 0.05) in ZnSO4 group. The results indicate that CS-Zn is more effective in serum zinc accumulation, and it might regulate zinc homeostasis by affecting zinc transporter mRNA expression and absorption mechanism might be different with ZnSO4.

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Acknowledgments

The authors thank Bojing Liu for her skillful technical assistance. This work was financed by the National Science Foundation (31272477).

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Authors and Affiliations

  1. Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China

    Mengyuan Lv, Xiongfeng Fu, Luansha Hu, Xiaojing Yue & Xinyan Han

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  1. Mengyuan Lv
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  2. Xiongfeng Fu
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  3. Luansha Hu
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  4. Xiaojing Yue
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  5. Xinyan Han
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Correspondence to Xinyan Han.

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Lv, M., Fu, X., Hu, L. et al. The Expression of Zinc Transporters Changed in the Intestine of Weaned Pigs Exposed to Zinc Chitosan Chelate. Biol Trace Elem Res 174, 328–334 (2016). https://doi.org/10.1007/s12011-016-0732-1

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  • DOI: https://doi.org/10.1007/s12011-016-0732-1

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