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Influence of Cobalt and Zinc Exposure on mRNA Expression Profiles of Metallothionein and Cytocrome P450 in Rainbow Trout

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Abstract

The present research aims to evaluate the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on metallothioneins and cytocrome P450. Mature rainbow trouts were exposed to 10 mg/L CoCl2.6H2O and 1 mg/L ZnSO4.7H2O. After 6, 12, 24, and 48 h of treatment, expressions of muscle MT-A, MT-B, and CYP P4501A1 mRNAs were measured by means of quantitative real-time polymerase chain reaction. During the exposure experiments, no mortalities occurred. We observed that expression levels of all genes increased with exposure time. Since the organism has not learned how to completely dispose of heavy metals and tends to bioaccumulate them, our results indicate that cobalt and zinc exposure may result in accumulation of the non-eliminated metals which may lead to fish death.

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Authors and Affiliations

  1. Hınıs Vocational Training School, Atatürk University, 25600, Erzurum, Turkey

    Saltuk Buğrahan Ceyhun

  2. Biotechnology Research Center, Atatürk University, 25240, Erzurum, Turkey

    Saltuk Buğrahan Ceyhun, Orhan Erdoğan & Şükrü Beydemir

  3. Agriculture Faculty, Department of Agricultural Biotechnology, Atatürk University, 25240, Erzurum, Turkey

    Ercüment Aksakal

  4. Agriculture Faculty, Department of Agricultural Biotechnology, Ondokuz Mayıs University, 55200, Samsun, Turkey

    Deniz Ekinci

  5. Science Faculty, Department of Molecular Biology and Genetics, Atatürk University, 25240, Erzurum, Turkey

    Orhan Erdoğan

  6. Science Faculty, Chemistry Department, Atatürk University, 25240, Erzurum, Turkey

    Şükrü Beydemir

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  1. Saltuk Buğrahan Ceyhun
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  2. Ercüment Aksakal
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Correspondence to Saltuk Buğrahan Ceyhun.

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Ceyhun, S.B., Aksakal, E., Ekinci, D. et al. Influence of Cobalt and Zinc Exposure on mRNA Expression Profiles of Metallothionein and Cytocrome P450 in Rainbow Trout. Biol Trace Elem Res 144, 781–789 (2011). https://doi.org/10.1007/s12011-011-9068-z

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