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Reversal of Osteoporotic Changes of Mineral Composition in Femurs of Diabetic Rats by Insulin

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Abstract

Insulin plays an important role in bone prevention of diabetic osteoporosis, but little is known about the relation between the bone mineral density (BMD) increase and the change of mineral element content after treated with insulin. To address this problem, male Wistar rats were randomly divided into three groups: normal group (n = 6), streptozotocin-induced diabetic group (n = 5), and streptozotocin-induced diabetic group with insulin treatment (n = 5). The femoral BMD was measured by dual energy X-ray absorptiometry, and the element content was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The results showed that the femoral BMD in diabetic group was significantly lower than that in normal group (P < 0.01) but restored by insulin treatment (P < 0.01 vs diabetic group). ICP-AES analysis revealed that the element content of calcium (Ca), phosphorous (P), magnesium (Mg), strontium (Sr), and potassium (K) in diabetic group were remarkably lower than those in normal group (P < 0.01) but only Ca, P, and Mg content were significantly increased compared with diabetic group (P < 0.05) after insulin treatment. However, no significant differences were observed in element zinc (Zn) content among three groups. Our findings suggested that the loss of Ca, P, Mg, Sr, and K content accounted for the lower BMD in streptozotocin-induced diabetes rats, insulin treatment could restore BMD by increasing the content of Ca, P, and Mg.

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Acknowledgement

This work is supported by the Scientific Research Foundation of Graduate University of Chinese Academy of Sciences (No. 055101FM03) and China National Natural Sciences Foundation (No. 20571084).

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  1. School of Life Sciences, Graduate University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, People’s Republic of China

    Xiaolin Zhang, Yurong Fei, Min Zhang, Dan Wei, Ming Li, Wenjun Ding & Jianhong Yang

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  1. Xiaolin Zhang
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  2. Yurong Fei
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Correspondence to Jianhong Yang.

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Zhang, X., Fei, Y., Zhang, M. et al. Reversal of Osteoporotic Changes of Mineral Composition in Femurs of Diabetic Rats by Insulin. Biol Trace Elem Res 121, 233–242 (2008). https://doi.org/10.1007/s12011-007-8043-1

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  • DOI: https://doi.org/10.1007/s12011-007-8043-1

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