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Diagnostic and Prognostic Values of KLF5 and RUNX1 in Acute Kidney Injury in Septic Patients

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Abstract

Diagnostic and prognostic values of Kruppel-like factor 5 (KLF5) and Runt-related transcription factor 1 (RUNX1) were determined in sepsis-induced acute kidney injury (SI-AKI). The study included 120 septic patients and set two groups: SI-AKI group (n = 60) or non-AKI group (n = 60). Fasting venous blood was drawn, and KLF5 and RUNX1 levels were measured. The receiver operating characteristic curve was plotted for diagnostic evaluation of KLF5 and RUNX1 in SI-AKI. The correlation between KLF5 and RUNX1 and serum creatinine (Scr), cystatin C (Cys-C), and kidney injury molecule 1 (KIM-1) were assessed by Pearson method. Predictive values of KLF5 and RUNX1 in 28-day survival of SI-AKI patients were considered by Kaplan-Meier survival curves and multivariate Cox regression analysis. Serum KLF5 and RUNX1 in SI-AKI patients were upregulated. Serum KLF5 and RUNX1 were of high diagnostic value in distinguishing SI-AKI patients from non-AKI patients. KLF5 and RUNX1 were in a positive correlation with Scr, Cys-C, and KIM-1, respectively. The 28-day survival of SI-AKI patients with high serum KLF5 or RUNX1 expression was poor, and serum KLF5 and RUNX1 expression were independently correlated with SI-AKI patients’ survival. KLF5 and RUNX1 have diagnostic and prognostic values in SI-AKI patients.

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Data Availability

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Medical ICU, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou City, 510630, Guangdong Province, China

    JiaJia Hu, YuSen Lin, YanHong Wang, YunFeng Shi, XiaoHan Shi & BenQuan Wu

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  1. JiaJia Hu
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  2. YuSen Lin
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  3. YanHong Wang
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  4. YunFeng Shi
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Contributions

J Hu designed the research study. Y Lin and Y Wang performed the research. Y Shi and X Shi provided help and advice on the experiments. J Hu and B Wu analyzed the data. J Hu wrote the manuscript. B Wu reviewed and edited the manuscript. All authors contributed to editorial changes in the manuscript. All authors read and approved the final manuscript.

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Correspondence to BenQuan Wu.

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Ethics Approval and Consent to Participate

The present study was approved by the Ethics Committee of The Third Affiliated Hospital of Sun Yat-sen University, and written informed consent was provided by all patients prior to the study start. All procedures were performed in accordance with the ethical standards of the Institutional Review Board and The Declaration of Helsinki, and its later amendments or comparable ethical standards.

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Written informed consent for publication was obtained from all participants.

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The authors declare no competing interests.

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Supplementary Information

ESM 1

Supplementary Figure 1 Results of bioinformatics analysis. A: Differentially expressed genes between Non-AKI and SI-AKI; B-C: KLF5 and RUNX1 were significantly upregulated in SI-AKI. (TIF 3430 kb)

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Hu, J., Lin, Y., Wang, Y. et al. Diagnostic and Prognostic Values of KLF5 and RUNX1 in Acute Kidney Injury in Septic Patients. Appl Biochem Biotechnol (2024). https://doi.org/10.1007/s12010-024-04956-w

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