Abstract
With the global population explosion, the need for increasing crop productivity is reaching its peak. The significance of organic means of cultivation including biofertilizers and biopesticides is undeniable in this context. Over the last few decades, the use of rhizobacteria to induce crop productivity has gained particular interest of researchers. Of these, several Bacillus spp. have been known for their potential plant growth-promoting and phyto-pathogenic actions. Keeping this background in mind, this study was formulated with an aim to unravel the PGPR and phyto-pathogenic potency of Bacillus sp. isolated from extreme environmental conditions, viz. high-altitude waters of Ganges at Gangotri (Basin Extent Longitude Latitude—73° 2′ to 89° 5′ E 21° 6′ to 31° 21′ N). Based on recent studies showing the impact of biofilm on bacterial PGPR potency, three novel strains of Bacillus subtilis were isolated on basis of their extremely high biofilm-producing abilities (BRAM_G1: Accession Number MW006633; BRAM_G2: Accession Numbers MT998278-MT998280; BRAM_G3: Accession Number MT998617), and were tested for their PGPR properties like nutrient sequestration, growth hormone production (IAA, GA3), stress-responsive enzyme production (ACC deaminase) and lignocellulolytic and agriculturally important enzyme productions. The strains were further tested for the plethora of metabolites (liquid and VOCs) exuded by them. Finally, the strains both in individually and in an association, i.e. consortium was tested on a test crop, viz. Zea mays L., and the data were collected at regular intervals and the results were statistically analysed. In the present study, the role of high-altitude novel Bacillus subtilis strains as potent PGPR has been analysed statistically.
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References
Ahemad, M., & Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University Science, 26, 1–20. https://doi.org/10.1016/j.jksus.2013.05.001
Holbrook, A. A., Edge, W. J. W., & Bailey, F. (1961) Spectrophotometric method for determination of gibberellic acid. Gibberellins, Advances in Chemistry, American Chemical Society, pp. 159–167
Arora, N. K., Mehnaz, S., & Balestrini, R. (2016). Bioformulations: For sustainable agriculture. Springer. https://doi.org/10.1016/j.mib.2017.03.011
Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., Smith, D. L. (2018). Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 9, https://doi.org/10.3389/fpls.2018.01473
Bailly, A., & Weisskopf, L. (2012). The modulating effect of bacterial volatiles on plant growth: Current knowledge and future challenges. Plant Signaling & Behavior, 7, 79–85. https://doi.org/10.4161/psb.7.1.18418
Roy, B., Maitra, D., Mitra, A.K. (2021). Methods of sample preparation and assay of bacterial biofilms with special reference to their significance in agriculture and extreme environments. In: Nag, M., Lahiri, D. (eds), Analytical Methodologies for Biofilm Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1378-8_2
Roy, B., Maitra, D., Chandra, A., Ghosh, J., & Mitra, A. K. (2022). Biofilm production in a novel polyextremophilic Bacillus subtilis: A strategic maneuver for survival. Biocatalysis and Agricultural Biotechnology, 45, 102517. https://doi.org/10.1016/j.bcab.2022.102517. ISSN 1878-8181.
Glick, B. R. (2005). Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS Microbiology Letters, 251(1), 1–7. https://doi.org/10.1016/j.femsle.2005.07.030
Borriss, R. (2011). Use of plant-associated bacillus strains as biofertilizers and biocontrol agents in agriculture. In: Maheshwari, D. (eds), Bacteria in Agrobiology: Plant Growth Responses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20332-9_3
Chaudhari, D. S., Dhotre, D. P., Jani, K., et al. (2020). Bacterial communities associated with the biofilms formed in high-altitude brackish water Pangong Tso located in the Himalayan plateau. Current Microbiology, 77, 4072–4084. https://doi.org/10.1007/s00284-020-02244-4
Maitra, D., Roy, B., Chandra, A., Choudhury, S. S., & Mitra, A. K. (2022). Biofilm producing Bacillus vallismortis TR01K from tea rhizosphere acting as plant growth promoting agent. Biocatalysis and Agricultural Biotechnology, 45, 102507. https://doi.org/10.1016/j.bcab.2022.102507. ISSN 1878-8181.
Mekonnen, E., Kebede, A., Nigussie, A., Kebede, G., & Tafesse, M. (2021). Isolation and characterization of urease-producing soil bacteria”. International Journal of Microbiology, 2021(8888641), 11. https://doi.org/10.1155/2021/8888641
Fahad, S., Hussain, S., Bano, A., Saud, S., Hassan, S., Shan, D., et al. (2015). Potential role of phytohormones and plant growth-promoting rhizobacteria in abiotic stresses: Consequences for changing environment. Environmental Science and Pollution Research, 22, 4907–4921. https://doi.org/10.1007/s11356-014-3754-2
Zannier, F., Portero, L. R., Ordoñez, O. F., Martinez, L. J., Farías, M. E., & Albarracin, V. H. (2019). Polyextremophilic bacteria from high altitude andean lakes: Arsenic resistance profiles and biofilm production. BioMed Research International, 2019(1231975), 11. https://doi.org/10.1155/2019/1231975
Jha, C. K., & Saraf, M. (2011). In vitro evaluation of indigenous plant growth promoting rhizobacteria isolated from Jatropha curcas rhizosphere. International Journal of Genetic Engineering and Biotechnology, 2, 91–100.
Kasim, W. A., Gaafar, R. M., Abou-Ali, R. M., Omar, M. N., & Hewait, H. M. (2016). Effect of biofilm forming plant growth promoting rhizobacteria on salinity tolerance in barley. Annals of Agricultural Science, 61, 217–227.
Mah, T. F., Pitts, B., Pellock, B., Walker, G. C., Stewart, P. S., & O’toole, G. A. (2003). A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance. Nature, 426, 306–310. https://doi.org/10.1038/nature02122
Malusá E, Sas-Paszt L, Ciesielska J. (2012). Technologies for beneficial microorganisms inocula used as biofertilizers. Scientific World Journal, 2012, 491206. https://doi.org/10.1100/2012/491206.
Yazdani, M., Bahmanyar, M.A., Pirdashti, H., & AliEsmaili, M. (2009). Effect of Phosphate Solubilization Microorganisms (PSM) and Plant Growth Promoting Rhizobacteria (PGPR) on Yield and Yield Components of Corn (Zea mays L.). World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 3, 50–52.
Pahari, A., Pradhan, A., Nayak, S.K., Mishra, B.B. (2017). Bacterial siderophore as a plant growth promoter. In: Patra, J., Vishnuprasad, C., Das, G. (eds), Microbial Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-10-6847-8_7
Pandea, A., Kaushik, S., Pandey, P., & Negi, A. (2019). Isolation, characterization, and identification of phosphate-solubilizing Burkholderia cepacia from the sweet corn cv. Golden Bantam rhizosphere soil and effect on growth-promoting activities. International Journal of Vegetable Science. https://doi.org/10.1080/19315260.2019.1692121. Taylor & Francis.
Pandit, A., Adholeya, A., Cahill, D., Brau, L., & Kochar, M. (2020). Microbial biofilms in nature: Unlocking their potential for agricultural applications. Journal of Applied Microbiology, 129(2), 199–211.
Radhakrishnan, M., Samshath, K. J., & Balagurunathan, R. (2014). Hydroxamate siderophore from Bacillus spSD12 isolated from iron factory soil. Current World Environment: An International Research Journal of Environmental Sciences, 9(3), 990–993.
Reddy, E.C. et al. (2022). Hydrolytic enzyme producing Plant Growth-Promoting Rhizobacteria (PGPR) in plant growth promotion and biocontrol. In: Sayyed, R.Z., Uarrota, V.G. (eds), Secondary Metabolites and Volatiles of PGPR in Plant-Growth Promotion. Springer, Cham. https://doi.org/10.1007/978-3-031-07559-9_15
Pii, Y., Mimmo, T., Tomasi, N., Terzano, R., Cesco, S., & Crecchio, C. (2015). Microbial interactions in the rhizosphere: Beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review. Biology and Fertility of Soils, 51, 403–415. https://doi.org/10.1007/s00374-015-0996-1
Santoyo, G., Orozco-Mosqueda, M. D., & Govindappa, M. (2012). Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: A review. Biocontrol Science and Technology, 22, 855–872. https://doi.org/10.1016/j.micres.2008.08.007
Schwyn, B., & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160(1), 47–56. https://doi.org/10.1016/0003-2697(87)90612-9
Shaikh, S. S., Wani, S. J., & Sayyed, R. Z. (2018). Impact of interactions between rhizosphere and rhizobacteria: A review. Journal of Bacteriology and Mycology, 5, 1058.
Shivlata, L., Satyanarayana, T. (2017). Actinobacteria in agricultural and environmental sustainability. In: Singh, J., Seneviratne, G. (eds), Agro-Environmental Sustainability. Springer, Cham. https://doi.org/10.1007/978-3-319-49724-2_9
Sivasakthi, S., Usharani, G., & Saranraj, P. (2014). Biocontrol potentiality of plant growth promoting bacteria (PGPR)-Pseudomonas fluorescens and Bacillus subtilis: A review. African Journal of Agricultural Research, 9, 1265–1277.
Subba Rao, N. S. (1977). Soil microorganisms and plant growth. Oxford and IBH Publishing Co.
Timmusk, S., Behers, L., Muthoni, J., Muraya, A., & Aronsson, A. C. (2017). Perspectives and challenges of microbial application for crop improvement. Frontiers in Plant Science, 8, 49. https://doi.org/10.3389/fpls.2017.00049
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255, 571–586. https://doi.org/10.1023/A:1026037216893
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Roy, B., Maitra, D., Biswas, A. et al. Efficacy of High-Altitude Biofilm-Forming Novel Bacillus subtilis Species as Plant Growth-Promoting Rhizobacteria on Zea mays L. Appl Biochem Biotechnol 196, 643–666 (2024). https://doi.org/10.1007/s12010-023-04563-1
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DOI: https://doi.org/10.1007/s12010-023-04563-1