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Epidural Administration of Curcumin-Loaded Polycaprolactone/Gelatin Electrospun Nanofibers for Extended Analgesia After Laminectomy in Rats

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Abstract

Several clinical studies have reported the analgesic effect of curcumin (Curc) in various situations such as rheumatoid arthritis, osteoarthritis, and postsurgical pain. Therefore, in this work, Curc-loaded electrospun nanofibers (NFs) are designed to evaluate their sustained release on analgesic effect duration in rats after epidural placement via repeated formalin and tail-flick tests. The Curc-loaded polycaprolactone/gelatin NFs (Curc-PCL/GEL NFs) are prepared through an electrospinning technique and introduced to the rat’s epidural space after laminectomy. The physicochemical and morphology features of the prepared Curc-PCL/GEL NFs were characterized via FE-SEM, FTIR, and degradation assay. The in vitro and in vivo concentrations of Curc were measured to evaluate the analgesic efficacy of the drug-loaded NFs. Rat nociceptive responses are investigated through repeated formalin and tail-flick tests for 5 weeks after the placement of NFs. Curc had a sustained release from the NFs for 5 weeks, and its local pharmaceutical concentrations were much greater than plasma concentrations. Rat’s pain scores in both early and late phases of the formalin test were remarkably decreased in the experimental period. Rat’s tail-flick latency was remarkably enhanced and remained constant for up to 4 weeks. Our findings show that the Curc-PCL/GEL NFs can supply controlled release of Curc to induce extended analgesia after laminectomy.

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Data Availability

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

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Acknowledgements

The authors would like to thank the “Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran” for their kind cooperation.

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Authors and Affiliations

  1. Department of Anesthesiology, 3201 Hospital, Hanzhong, 723000, China

    Tingting Jiang & Yu Han

  2. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Niloufar Esmaeilizadeh

  3. Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran

    Amin Barkhordari

  4. Medical Laboratory Techniques Department, Al-Mustaqbal University College, Babylon, Hilla, 51001, Iraq

    Abduladheem Turki Jalil

  5. Department of Biophysics, College of Applied Sciences, University of Anbar, Anbar, Iraq

    Marwan Mahmood Saleh

  6. Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq

    Marwan Mahmood Saleh

  7. Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran

    Sona Talaei

  8. Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran

    Younes Pilehvar

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Contributions

TJ and YH: methodology, investigation, original draft preparation. NE: conceptualization, investigation, resources. AB: investigation, methodology, validation. ATJ: formal analysis, writing–review and editing. MMS: methodology, writing–review and editing. ST: project administration. YP: supervision, writing–review and editing, funding acquisition.

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Correspondence to Younes Pilehvar.

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All procedures performed in studies involving human participants were under the ethical standards of the Ethics Committee of Tabriz University of Medical Sciences and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Tingting Jiang and Yu Han are co-first authors (these authors contributed equally to this work).

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Jiang, T., Han, Y., Esmaeilizadeh, N. et al. Epidural Administration of Curcumin-Loaded Polycaprolactone/Gelatin Electrospun Nanofibers for Extended Analgesia After Laminectomy in Rats. Appl Biochem Biotechnol 195, 6557–6571 (2023). https://doi.org/10.1007/s12010-023-04342-y

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