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Overexpression of Dioxygenase Encoding Gene Accelerates the Phenolic Aldehyde Conversion and Ethanol Fermentability of Zymomonas mobilis

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Abstract

NADH-dependent reductase enzyme catalyzes the phenolic aldehyde conversion and correspondingly improves the ethanol fermentability of the ethanologenic Zymomonas mobilis. This study constructed the transcriptional landscape of mono/dioxygenase genes in Z. mobilis ZM4 under the stress of the toxic phenolic aldehyde inhibitors of 4-hydroxybenzaldehyde, syringaldehyde, and vanillin. One specific dioxygenase encoding gene ZMO1721 was differentially expressed by 3.07-folds under the stress of 4-hydroxybenzaldehyde among the eleven mono/dioxygenase genes. The purified ZMO1721 shared 99.9% confidence and 48.0% identity with the oxidoreductase in Rhodoferax ferrireducens T118 was assayed and the NADH-dependent reduction activity was confirmed for phenolic aldehyde vanillin conversion. The ZMO1721 gene was then overexpressed in Z. mobilis ZM4 and the 4-hydroxybenzaldehyde conversion rate was accelerated. The cell growth, glucose consumption, and ethanol productivity of Z. mobilis ZM4 were also improved by ZMO1721 overexpression. The genes identified on improving phenolic aldehyde tolerance and ethanol fermentability in this study could be used as the synthetic biology tools for modification of ethanologenic strains.

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Availability of Data and Materials

The data and materials that support the findings of this study are available from the corresponding author upon reasonable request.

Funding

This research was supported by Natural Science Foundation of Jiangxi (20192BAB204002), Open Funding Project of the State Key Laboratory of Bioreactor Engineering, and Doctor Science Research Foundation of Jiujiang University (8879524).

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Authors and Affiliations

  1. Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China

    Xia Yi, Jun Mei & Ling Lin

  2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Wei Wang

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XY and WW designed the experiment and drafted the manuscript. XY, JM, and LL carried out the experiment. WW and XY were in charge of the overall project. All authors read and approved to publish the final manuscript.

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Correspondence to Xia Yi.

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Yi, X., Mei, J., Lin, L. et al. Overexpression of Dioxygenase Encoding Gene Accelerates the Phenolic Aldehyde Conversion and Ethanol Fermentability of Zymomonas mobilis. Appl Biochem Biotechnol 193, 3017–3027 (2021). https://doi.org/10.1007/s12010-021-03551-7

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