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Capability Enhancement of Fumaric Acid Production by Rhizopus arrhizus Through Carbon-Nitrogen Sources Coordination

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Abstract

Fumaric acid production from the fermentation process by Rhizopus was considered a potential method. But poor conversion efficiency and low space-time productivity greatly hampered industrial production. Here, we reported improving these problems through carbon-nitrogen sources coordination optimization strategy. Five commonly used nitrogen sources were selected to conduct element analysis and fermentation efficiency comparison. Casein was proven to be the optimum nitrogen source and further investigated in a stirred-tank reactor. It showed that the fermentation cycle was significantly shortened by the application of casein. Combined with optimization of glucose content, the space-time productivity of fumaric acid reached 0.76 g/L h with a yield to 0.31 g/g glucose, which was the highest among the results gotten in the stirred-tank reactor. It illustrated that carbon-nitrogen sources coordination optimization strategy was in favor of the improvement of the fermentation process and laid a promising foundation for the development of fumaric acid industrial production.

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Funding

This research was financially supported by the National Key Research Program (2016YFD0400601), National Natural Science Foundation of China (21978019), China Postdoctoral Science Foundation (2019M660420), Petro China Innovation Foundation (H2018415), and Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009).

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Authors and Affiliations

  1. Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Hao Xing, Huan Liu, Yakun Zhang, Yue Yu, Xiaolan Huang, Qi Xiao, Li Deng & Fang Wang

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  1. Hao Xing
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  2. Huan Liu
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  3. Yakun Zhang
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  4. Yue Yu
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  5. Xiaolan Huang
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  6. Qi Xiao
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  7. Li Deng
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  8. Fang Wang
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Correspondence to Li Deng.

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Xing, H., Liu, H., Zhang, Y. et al. Capability Enhancement of Fumaric Acid Production by Rhizopus arrhizus Through Carbon-Nitrogen Sources Coordination. Appl Biochem Biotechnol 193, 1231–1237 (2021). https://doi.org/10.1007/s12010-020-03461-0

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  • DOI: https://doi.org/10.1007/s12010-020-03461-0

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