Abstract
A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), and pure algae, were investigated. All feeds were consumed by zebrafish and showed optimal growth and weight gain with a survival rate of 100%. Lipids were extracted from four different tissues, brain, liver, muscle, and blood, to understand the distribution of fatty acids with respect to the feed. Maximum lipid was observed in zebrafish fed with 50:50 feed in all tissue samples. An increasing concentration of fatty acids was observed upon increasing the experimental time. Algae feed supported the DHA accumulation in all tissue samples compared to other feeds and resulted in an overall increment of polyunsaturated fatty acid content. To understand the role of fatty acids during zebrafish embryogenesis, eggs were collected at the end of the experiment and fatty acid content was analyzed. However, no significant difference was observed in fatty acid composition of embryos fed with algae. This provides a base for the understanding of fatty acid distribution in zebrafish with commercial and algae feeds and support the utilization of Schizochytrium biomass as a potential replacement for fishmeal.
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Maehre, H., Jensen, I.-J., Elvevoll, E., & Eilertsen, K.-E. (2015). ω-3 fatty acids and cardiovascular diseases: effects, mechanisms and dietary relevance. International Journal of Molecular Sciences, 16(9), 22636–22661.
McManus, A., Hunt, W., Storey, J., McManus, J., & Hilhorst, S. (2014). Perceptions and preference for fresh seafood in an Australian context. International Journal of Consumer Studies, 38(2), 146–152.
Pontecorvo, G., & Schrank, W. E. (2012). The expansion, limit and decline of the global marine fish catch. Marine Policy, 36(5), 1178–1181.
Thurstan, R. H., & Roberts, C. M. (2014). The past and future of fish consumption: can supplies meet healthy eating recommendations? Marine Pollution Bulletin, 89(1–2), 5–11.
Cabral, E. M., et al. (2013). Replacement of fish meal by plant protein sources up to 75% induces good growth performance without affecting flesh quality in ongrowing Senegalese sole. Aquaculture, 380–383, 130–138.
Turchini, G. M., Torstensen, B. E., & Ng, W.-K. (2009). Fish oil replacement in finfish nutrition. Reviews in Aquaculture, 1(1), 10–57.
Badvipour, S., Eustance, E., & Sommerfeld, M. R. (2016). Process evaluation of energy requirements for feed production using dairy wastewater for algal cultivation: theoretical approach. Algal Research, 19, 207–214.
Vaz, B. d. S., Moreira, J. B., Morais, M. G. d., & Costa, J. A. V. (2016). Microalgae as a new source of bioactive compounds in food supplements. Current Opinion in Food Science, 7, 73–77.
Byreddy, A. R. (2016). Thraustochytrids as an alternative source of omega-3 fatty acids, carotenoids and enzymes. Lipid Technology, 28(3–4), 68–70.
Gupta, A., Singh, D., Byreddy, A. R., Thyagarajan, T., Sonkar, S. P., Mathur, A. S., Tuli, D. K., Barrow, C. J., & Puri, M. (2016). Exploring omega-3 fatty acids, enzymes and biodiesel producing thraustochytrids from Australian and Indian marine biodiversity. Biotechnology Journal, 11(3), 345–355.
García-Ortega, A., Kissinger, K. R., & Trushenski, J. T. (2016). Evaluation of fish meal and fish oil replacement by soybean protein and algal meal from Schizochytrium limacinum in diets for giant grouper Epinephelus lanceolatus. Aquaculture, 452, 1–8.
Ulloa, P. E., Iturra, P., Neira, R., & Araneda, C. (2011). Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes. Reviews in Fish Biology and Fisheries, 21(4), 649–666.
Byreddy, A. R., Barrow, C. J., & Puri, M. (2016). Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase. Bioresource Technology, 200, 464–469.
Yoganantharjah, P., Byreddy, A. R., Fraher, D., Puri, M., & Gibert, Y. (2017). Rapid quantification of neutral lipids and triglycerides during zebrafish embryogenesis. International Journal of Developmental Biology, 61(1-2), 105–111.
Pedroso, G. L., Hammes, T. O., Escobar, T. D., Fracasso, L. B., Forgiarini, L. F., da Silveira, T. R. (2012). Blood collection for biochemical analysis in adult zebrafish. Journal of Visualized Experiments, 63, e3865.
Gupta, T., & Mullins, M. C. (2010). Dissection of organs from the adult zebrafish. Journal of Visualized Experiments, 37, 1717.
Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(1), 911–917.
Sarker, P. K., Kapuscinski, A. R., Lanois, A. J., Livesey, E. D., Bernhard, K. P., & Coley, M. L. (2016). Towards sustainable aquafeeds: complete substitution of fish oil with marine microalga Schizochytrium sp. improves growth and fatty acid deposition in juvenile Nile tilapia (Oreochromis niloticus). PLoS One, 11(6), e0156684.
Kousoulaki, K., Mørkøre, T., Nengas, I., Berge, R. K., & Sweetman, J. (2016). Microalgae and organic minerals enhance lipid retention efficiency and fillet quality in Atlantic salmon (Salmo salar L.). Aquaculture, 451, 47–57.
Glencross, B., & Rutherford, N. (2011). A determination of the quantitative requirements for docosahexaenoic acid for juvenile barramundi (Lates calcarifer). Aquaculture Nutrition, 17(2), e536–e548.
Van Hoestenberghe, S., et al. (2016). Schizochytrium as a replacement for fish oil in a fishmeal free diet for jade perch, Scortum barcoo (McCulloch & Waite). Aquaculture Research, 47(6), 1747–1760.
Hong, H., Zhou, Y., Wu, H., Luo, Y., & Shen, H. (2014). Lipid content and fatty acid profile of muscle, brain and eyes of seven freshwater fish: a comparative study. Journal of the American Oil Chemists' Society, 91(5), 795–804.
Norambuena, F., Hermon, K., Skrzypczyk, V., Emery, J. A., Sharon, Y., Beard, A., & Turchini, G. M. (2015). Algae in fish feed: performances and fatty acid metabolism in juvenile Atlantic salmon. PLoS One, 10(4), e0124042.
Van Hoestenberghe, S., Roelants, I., Vermeulen, D., & Goddeeris, B. M. (2013). Total replacement of fish oil with vegetable oils in the diet of juvenile jade perch scortum barcoo reared in recirculating aquaculture systems. Journal of Agricultural Science and Technology, 5B, 385–398.
Bureau, D. P., Hua, K., & Harris, A. M. (2008). The effect of dietary lipid and long-chain n-3 PUFA levels on growth, energy utilization, carcass quality, and immune function of rainbow trout, Oncorhynchus mykiss. Journal of the World Aquaculture Society, 39(1), 1–21.
Suloma A, Ogata H., Garibay ES, Chavez DR, El-Haroun ER (2008) Fatty acid composition of Nile Tilapia Oreochromis niloticus muscles: a comparative study with commercially important tropical freshwater fish in Philippines, in 8th International Symposium on Tilapia in Agriculture. Egypt Ministry of Agriculture: Cairo, p. 921–932.
Jezierska, B., Hazel, J. R., & Gerking, S. D. (1982). Lipid mobilization during starvation in the rainbow trout, Salmo gairdneri Richardson, with attention to fatty acids. Journal of Fish Biology, 21(6), 681–692.
Kousoulaki, K., et al. (2015). Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae. Journal of Nutritional Science, 4, e24.
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The authors would like to thank the Deakin University zebrafish facility for providing excellent husbandry care. AB and PY acknowledge the support of Deakin scholarship.
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Byreddy, A.R., Yoganantharjah, P., Gupta, A. et al. Suitability of Novel Algal Biomass as Fish Feed: Accumulation and Distribution of Omega-3 Long-Chain Polyunsaturated Fatty Acid in Zebrafish. Appl Biochem Biotechnol 188, 112–123 (2019). https://doi.org/10.1007/s12010-018-2906-0
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DOI: https://doi.org/10.1007/s12010-018-2906-0