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Enhanced Butanol Production Through Adding Organic Acids and Neutral Red by Newly Isolated Butanol-Tolerant Bacteria

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Abstract

As alternative microorganisms for butanol production with high butanol tolerant and productivity are in high demand, one excellent butanol-tolerant bacterium, S10, was isolated and identified as Clostridium acetobutylicum S10. In order to enhance the performance of butanol production, organic acids and neutral red were added during butanol fermentation. Synergistic effects were exhibited in the combinations of organic acids and neutral red to promote butanol production. Consequently, the optimal concentrations of combined acetate, butyrate, and neutral red were determined at sodium acetate 1.61 g/L, sodium butyrate 1.88 g/L, and neutral red 0.79 g/L, respectively, with the butanol yield of 6.09 g/L which was 20.89 % higher than that in control. These results indicated that combination of adding organic acid and neutral red is a potential effective measure to improve butanol production.

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Acknowledgment

This research was supported by the National Science and Technology Ministry (No. 2014BAJ21B02), National High Technology Research and Development Program (2011AA10A205), and the “Twelfth Five-Year Plan” National Science and Technology Program on Rural Area (2014BAL02B00) and Fundamental Research Funds for the Central Universities (No. HIT. KISTP. 201424).

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Authors and Affiliations

  1. School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China

    Cheng Jiang, Guangli Cao, Zhenyu Wang, Ying Li, Jinzhu Song, Hua Cong & Qian Yang

  2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China

    Guangli Cao & Qian Yang

  3. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, 150001, China

    Junzheng Zhang

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  1. Cheng Jiang
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  2. Guangli Cao
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Correspondence to Qian Yang.

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Jiang, C., Cao, G., Wang, Z. et al. Enhanced Butanol Production Through Adding Organic Acids and Neutral Red by Newly Isolated Butanol-Tolerant Bacteria. Appl Biochem Biotechnol 180, 1416–1427 (2016). https://doi.org/10.1007/s12010-016-2176-7

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