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Bio-therapeutic Potential and Cytotoxicity Assessment of Pectin-Mediated Synthesized Nanostructured Cerium Oxide

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Abstract

In the present studies, renewable and nontoxic biopolymer, pectin, was extracted from Indian red pomelo fruit peels and used for the synthesis of cerium oxide nanoparticles (CeO2-NPs) having bio-therapeutic potential. The structural information of extracted pectin was investigated by FTIR and NMR spectroscopic techniques. Physicochemical characteristics of this pectin suggested its application in the synthesis of metal oxide nanoparticles. Using this pectin as a template, CeO2-NPs were synthesized by simple, one step and eco-friendly approach. The UV–Vis spectrum of synthesized CeO2-NPs exhibited a characteristic absorption peak at wavelength 345 nm, which can be assigned to its intrinsic band gap (3.59 eV) absorption. Photoluminescence measurements of CeO2-NPs revealed that the broad emission was composed of seven different bands. FTIR analysis ensured involvement of pectin in the formation and stabilization of CeO2-NPs. FT-Raman spectra showed a sharp Raman active mode peak at 461.8 cm−1 due to a symmetrical stretching mode of Ce–O vibration. DLS, FESEM, EDX, and XRD analysis showed that the CeO2-NPs prepared were polydispersed, spherical shaped with a cubic fluorite structure and average particle size ≤40 nm. These CeO2-NPs displayed broad spectrum antimicrobial activity, antioxidant potential, and non-cytotoxic nature.

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Acknowledgment

SNP and JSP are thankful to the UGC, New Delhi for UGS-BSR/RFSMS fellowships. PBC is grateful to the DST, New Delhi for INSPIRE fellowship. All the authors acknowledge the SAIF-Chandigarh for NMR spectroscopic analysis, SAIF-IIT Chennai for FT-Raman spectroscopic study and UGS-SAP-DRS III and DST-FIST for providing infrastructure at School of Life Sciences of North Maharashtra University, Jalgaon.

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  1. Department of Microbiology, School of Life Sciences, North Maharashtra University, Umavi Nagar, Post Box 80, Jalgaon, 425 001, India

    Sandeep N. Patil, Jayasinh S. Paradeshi & Bhushan L. Chaudhari

  2. Department of Polymer Science and Technology, University Institute of Chemical Technology, North Maharashtra University, Jalgaon, 425 001, India

    Prapti B. Chaudhari & Satyendra J. Mishra

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  1. Sandeep N. Patil
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  2. Jayasinh S. Paradeshi
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Correspondence to Bhushan L. Chaudhari.

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Patil, S.N., Paradeshi, J.S., Chaudhari, P.B. et al. Bio-therapeutic Potential and Cytotoxicity Assessment of Pectin-Mediated Synthesized Nanostructured Cerium Oxide. Appl Biochem Biotechnol 180, 638–654 (2016). https://doi.org/10.1007/s12010-016-2121-9

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