Skip to main content
SpringerLink
Log in

Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The extracellular lipase of Yarrowia lipolytica presents numerous potentialities for biotechnological applications. This work describes the development and storage of powders obtained from supernatants containing Y. lipolytica lipase by freeze-drying as downstream process that is important in obtaining a stable lipase powder with high enzymatic activity. Lipase was produced by Y. lipolytica U6 mutant strain in 20-L bioreactor. Non-concentrated cell-free culture supernatant samples were supplemented with different concentrations (0.5–1 %) of maltodextrin and glycerol as additives to freeze-drying. Effects of additives, temperature, pH, and storage time on lipase powders were determined. After addition of additives, freeze-drying yield increased 3.5-fold compared to supernatant without additive. Maltodextrin with 0.5 % concentration gave the best protection of lipase during dehydration treatment and its freeze-drying yield (77 %) is better than other formulations. Lipase powders were stored at 4 and 25 °C for 46 weeks without loss of lipase activity. A common impediment to the production of commercial enzyme is their low-stability aqueous solutions. The present study shows that freeze-dried lipase powders of Y. lipolytica have good stability for storage and various applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Barth, G., & Gaillardin, C. (1997). FEMS Microbiology Reviews, 19, 219–237.

    Article  CAS  Google Scholar 

  2. Fickers, P., Benetti, P. H., Wache, Y., Marty, A., Mauersberger, S., et al. (2005). FEMS Yeast Research, 5, 527–543.

    Article  CAS  Google Scholar 

  3. Darvishi, F., Nahvi, I., Zarkesh-Esfahani, H., & Momenbeik, F. (2009). Journal of Biomedicine and Biotechnology. doi:10.1155/2009/562943.

  4. Thevenieau, F., Le Dall, M. T., Nthangeni, B., Mauersberger, S., Marchal, R., et al. (2007). Fungal Genetics and Biology, 44, 531–542.

    Article  CAS  Google Scholar 

  5. Guieysse, D., Sandoval, G., Faure, L., Nicaud, J. M., Monsan, P., et al. (2004). Tetrahedron-Asymmetry, 15, 3539–3543.

    Article  CAS  Google Scholar 

  6. Cancino, M., Bauchart, P., Sandoval, G., Nicaud, J. M., André, I., et al. (2008). Tetrahedron-Asymmetry, 19, 1608–1612.

    Article  CAS  Google Scholar 

  7. Destain, J., Roblain, D., & Thonart, P. (1997). Biotechnology Letters, 19, 105–107.

    Article  CAS  Google Scholar 

  8. Pignede, G., Wang, H. J., Fudalej, F., Gaillardin, C., Seman, M., et al. (2000). Journal of Bacteriology, 182, 2802–2810.

    Article  CAS  Google Scholar 

  9. Fickers, P., Ongena, M., Destain, J., Weekers, F., & Thonart, P. (2006). Enzyme and Microbial Technology, 38, 756–759.

    Article  CAS  Google Scholar 

  10. Alloue, W. A. M., Destain, J., Ongena, M., Blecker, C., & Thonart, P. (2008). Preparative Biochemistry and Biotechnology, 38, 217–228.

    Article  CAS  Google Scholar 

  11. Mine, Y., Fukunaga, K., Samejima, K. I., Yoshimoto, M., Nakao, K., et al. (2003). Journal of Bioscience and Bioengineering, 96, 525–528.

    Article  CAS  Google Scholar 

  12. Yasuda, M., Kiguchi, T., Kasahara, H., Ogino, H., & Ishikawa, H. (2000). Journal of Bioscience and Bioengineering, 90, 681–683.

    CAS  Google Scholar 

  13. Werner, L., Latzko, F., & Hampel, W. (1993). Biotechnology Techniques, 7, 663–666.

    Article  CAS  Google Scholar 

  14. Aloulou, A., Rodriguez, J. A., Puccinelli, D., Mouz, N., Leclaire, J., et al. (2007). Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1771, 228–237.

    Article  CAS  Google Scholar 

  15. Adams, G. (2007). Methods in Molecular Biology., 368, 15–38.

    Article  CAS  Google Scholar 

  16. Millqvist-Fureby, A., Malmsten, M., & Bergenstahl, B. (1999). International Journal of Pharmacology, 188, 243–253.

    Article  CAS  Google Scholar 

  17. Dokic, P., Jakovljevic, J., & Dokic-Baucal, L. J. (1998). Colloids Surface A, 141, 435–440.

    Article  CAS  Google Scholar 

  18. Lovelock, J. E. (1953). Biochimica et Biophysica Acta, 11, 28–36.

    Article  CAS  Google Scholar 

  19. Darvishi, F., Destain, J., Nahvi, I., Thonart, P., & Zarkesh-Esfahani, H. (2011). New Biotechnology, 28, 756–760.

    Article  CAS  Google Scholar 

  20. Kar, T., Delvigne, F., Masson, M., Destain, J., & Thonart, P. (2008). Journal of Industrial Microbiology & Biotechnology, 35, 1053–1059.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Authors would like to thank Annick Salve, Martine Allegaert and Robin Dubois Dauphin for their help.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran

    Farshad Darvishi

  2. Department of Biology, Faculty of Science, University of Isfahan, Isfahan, 81746-73696, Iran

    Iraj Nahvi & Hamid Zarkesh-Esfahani

  3. Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Université de Liège, Gembloux Agro-Bio Tech, Passage des Déportés 2, 5030, Gembloux, Belgium

    Jacqueline Destain & Philippe Thonart

Authors
  1. Farshad Darvishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jacqueline Destain
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Iraj Nahvi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Philippe Thonart
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hamid Zarkesh-Esfahani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Farshad Darvishi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Darvishi, F., Destain, J., Nahvi, I. et al. Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase. Appl Biochem Biotechnol 168, 1101–1107 (2012). https://doi.org/10.1007/s12010-012-9844-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9844-z

Keywords

Search

Navigation