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Kinetics of Ergothioneine Inhibition of Mushroom Tyrosinase

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Abstract

The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC50 value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver–Burk plot of experimental kinetic data. The IC50 value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110 ± 0.305 mg/ml, much higher than the IC50 for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine.

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Acknowledgments

The authors thank the National Science Council, Taiwan, ROC for financial support (NSC99-2622-B-241-003-CC3).

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Authors and Affiliations

  1. Department of Nursing, Chang Gung Institution of Technology, Chia-Yi, Taiwan

    Wayne C. Liao

  2. Plastic and Reconstructive Cosmetic Surgery Center, Kuang Tien General Hospital, Taichung, Taiwan

    Wen Hong Wu

  3. Department of Applied Cosmetology, Hungkuang University, Shalu District, Taichung, 433, Taiwan

    Pei-Chuan Tsai, Hui-Feng Wang, Yi-Hsin Liu & Chin-Feng Chan

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  1. Wayne C. Liao
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  2. Wen Hong Wu
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Correspondence to Chin-Feng Chan.

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Liao, W.C., Wu, W.H., Tsai, PC. et al. Kinetics of Ergothioneine Inhibition of Mushroom Tyrosinase. Appl Biochem Biotechnol 166, 259–267 (2012). https://doi.org/10.1007/s12010-011-9421-x

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  • DOI: https://doi.org/10.1007/s12010-011-9421-x

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