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Gluconacetobacter hansenii subsp. nov., a High-Yield Bacterial Cellulose Producing Strain Induced by High Hydrostatic Pressure

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Abstract

Strain M438, deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-producing strain ever reported. In this paper, M438 was identidied as Gluconacetobacter hansenii subsp. nov. on the basis of the results obtained by examining it phylogenetically, phenotypically, and physiologically–biochemically. Furthermore, the genetic diversity of strain M438 and its initial strain was examined by amplified fragment length polymorphism. The results indicated that strain M438 was a deletion mutant induced by HHP, and the only deleted sequence showed 99% identity with 24,917–24,723 bp in the genome sequence of Ga. hansenii ATCC23769, and the complement gene sequence was at 24,699–25,019 bp with local tag GXY_15142, which codes small multidrug resistance (SMR) protein. It can be inferred that SMR might be related to inhibiting BC production to a certain extent.

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Abbreviations

CGMCC:

China General Microbiological Culture Collection

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Acknowledgments

The authors are thankful to Dr. Wu Ruiqin for technical support and Dr. Liu Liu for valuable suggestions. This work was supported by the Fundamental Research Funds for the Central Universities (QN2009072).

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Authors and Affiliations

  1. College of Food Science and Engineering, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 28 Xinong Road, Yangling, Xi’an, Shaanxi, 712100, China

    Han-Jing Ge, Shuang-Kui Du, De-Hui Lin, Jun-Na Zhang, Jin-Le Xiang & Zhi-Xi Li

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  1. Han-Jing Ge
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Correspondence to Zhi-Xi Li.

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Ge, HJ., Du, SK., Lin, DH. et al. Gluconacetobacter hansenii subsp. nov., a High-Yield Bacterial Cellulose Producing Strain Induced by High Hydrostatic Pressure. Appl Biochem Biotechnol 165, 1519–1531 (2011). https://doi.org/10.1007/s12010-011-9372-2

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  • DOI: https://doi.org/10.1007/s12010-011-9372-2

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