Abstract
This study presents a novel antibacterial surface comprised of Eucalyptus essential oil trapped within the micro-nanostructures of a polydimethylsiloxane (PDMS) substrate. A wettability study showed that the replication of the micro-nanostructure on PDMS led to a superhydrophobic behavior of the surface. The SEM images revealed that the micro-nanostructure increased the contact angle of the PDMS surfaces. Fourier transform infrared spectroscopy analysis led to the conclusion that the essential oil was entrapped in the PDMS, thereby promoting its antibacterial activity. Multiple tests demonstrate the antibacterial ability of these superhydrophobic surfaces in preventing the establishment of Escherichia coli and Bacillus cereus bacterial colonies and biofilms. These surfaces reduce contamination by 99.98% when exposed to a bacteria-rich water droplet. Our observations highlight the role of Eucalyptus essential oil in limiting initial bacterial growth and biofilm formation on exposed surfaces.
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References
Maillard, JY, Bloomfield, SF, Courvalin, P, Essack, SY, Gandra, S, Gerba, CP, Rubino, JR, Scott, EA, “Reducing Antibiotic Prescribing and Addressing the Global Problem of Antibiotic Resistance by Targeted Hygiene in the Home and Everyday Life Settings: A Position Paper.” Am. J. Infect. Control, 48 (9) 1090–1099. https://doi.org/10.1016/j.ajic.2020.04.011 (2020)
Forson, AM, van der Mei, HC, Sjollema, J, “Impact of Solid Surface Hydrophobicity and Micrococcal Nuclease Production on Staphylococcus aureus Newman Biofilms.” Sci. Rep., 10 12093. https://doi.org/10.1038/s41598-020-69084-x (2020)
Tremblay, YDN, Hathroubi, S, Jacques, M, “Les Biofilms Bactériens: Leur Importance en Santé Animale et en Santé Publique.” Can. J. Vet. Res., 78 110–116 (2014)
Li, YH, Tian, X, “Quorum Sensing and Bacterial Social Interactions in Biofilms.” Sensors, 12 (3) 2519–2538. https://doi.org/10.3390/s120302519 (2012)
Abdallah, M, Benoliel, C, Drider, D, Dhulster, P, Chihib, N-E, “Biofilm Formation and Persistence on Abiotic Surfaces in the Context of Food and Medical Environments.” Arch. Microbiol., 196 (7) 453–472. https://doi.org/10.1007/s00203-014-0983-1 (2014)
Douarche, C, Bailleux, V, Even, C, Allain, J-M, Regeard, C, Raspaud, E, “La Mécanique des Biofilms à la Surface de Liquides.” Reflets Phys., 56 20–24. https://doi.org/10.1051/refdp/201856020 (2018)
Flemming, HC, Meier, M, Schild, T, “Mini-Review: Microbial Problems in Paper Production.” Biofouling, 29 683–696. https://doi.org/10.1080/08927014.2013.798865 (2013)
Galié, S, García-Gutiérrez, C, Miguélez, EM, Villar, CJ, Lombó, F, “Biofilms in the Food Industry: Health Aspects and Control Methods.” Front. Microbiol., 9 898. https://doi.org/10.3389/fmicb.2018.00898 (2018)
Kanematsu, H, Barry, DM, Formation and Control of Biofilm in Various Environments. Springer Nature Singapore Pte. Ltd., Singapore (2020)
Avossa, J, Bifulco, A, Amendola, E, Gesuele, F, Oscurato, SL, Gizaw, Y, Mensitieri, G, Branda, F, “Forming Nanostructured Surfaces Through Janus Colloidal Silica Particles with Nanowrinkles: A New Strategy to Superhydrophobicity.” Appl. Surf. Sci., 465 73–81. https://doi.org/10.1016/j.apsusc.2018.09.131 (2019)
Bregnocchi, A, Jafari, R, Momen, G, “Design Strategies for Antiviral Coatings and Surfaces: A Review.” Appl. Surf. Sci. Adv., 8 100224. https://doi.org/10.1016/j.apsadv.2022.100224 (2022)
Gogolides, E, Ellinas, K, Tserepi, A, “Hierarchical Micro and Nano Structured, Hydrophilic, Superhydrophobic and Superoleophobic Surfaces Incorporated in Microfluidics, Microarrays and Lab on Chip Microsystems.” Microelectron. Eng., 132 135–155. https://doi.org/10.1016/j.mee.2014.10.002 (2015)
Zhang, X, Järn, M, Peltonen, J, Pore, V, Vuorinen, T, Levänen, E, Mäntylä, T, “Analysis of Roughness Parameters to Specify Superhydrophobic Antireflective Boehmite Films Made by the Sol-Gel Process.” J. Eur. Ceram. Soc., 28 (11) 2177–2181. https://doi.org/10.1016/j.jeurceramsoc.2008.02.020 (2008)
Polizos, G, Tuncer, E, Qiu, X, Aytug, T, Kidder, MK, Messman, JM, Sauers, I, “Nonfunctionalized Polydimethyl Siloxane Superhydrophobic Surfaces Based on Hydrophobic-Hydrophilic Interactions.” Langmuir, 27 (6) 2953–2957. https://doi.org/10.1021/la1042712 (2011)
Long, J, Pan, L, Fan, P, Gong, D, Jiang, D, Zhang, H, Li, L, Zhong, M, “Cassie-State Stability of Metallic Superhydrophobic Surfaces with Various Micro/Nanostructures Produced by a Femtosecond Laser.” Langmuir, 32 (4) 1065–1072. https://doi.org/10.1021/acs.langmuir.5b04329 (2016)
Privett, BJ, Youn, J, Hong, SA, Lee, J, Han, J, Shin, JH, Schoenfisch, MH, “Antibacterial Fluorinated Silica Colloid Superhydrophobic Surfaces.” Langmuir, 27 (15) 9597–9601. https://doi.org/10.1021/la201801e (2011)
Chauhan, P, Kumar, A, Bhushan, B, “Self-Cleaning, Stain-Resistant and Anti-Bacterial Superhydrophobic Cotton Fabric Prepared by Simple Immersion Technique.” J. Colloid Interface Sci., 535 66–74. https://doi.org/10.1016/j.jcis.2018.09.087 (2019)
Shateri Khalil-Abad, M, Yazdanshenas, ME, “Superhydrophobic Antibacterial Cotton Textiles.” J. Colloid Interface Sci., 351 (1) 293–298. https://doi.org/10.1016/j.jcis.2010.07.049 (2010)
Adlhart, C, Verran, J, Azevedo, NF, Olmez, H, Keinänen-Toivola, MM, Gouveia, I, Melo, LF, Crijns, F, “Surface Modifications for Antimicrobial Effects in the Healthcare Setting: A Critical Overview.” J. Hosp. Infect., 99 (3) 239–249. https://doi.org/10.1016/j.jhin.2018.01.018 (2018)
Ellinas, K, Kefallinou, D, Stamatakis, K, Gogolides, E, Tserepi, A, “Is There a Threshold in the Antibacterial Action of Superhydrophobic Surfaces?” ACS Appl. Mater. Interfaces, 9 (45) 39781–39789. https://doi.org/10.1021/acsami.7b11402 (2017)
Qian, H, Li, M, Li, Z, Lou, Y, Huang, L, Zhang, D, Xu, D, Du, C, Lu, L, Gao, J, “Mussel-Inspired Superhydrophobic Surfaces with Enhanced Corrosion Resistance and Dual-Action Antibacterial Properties.” Mater. Sci. Eng. C, 80 566–577. https://doi.org/10.1016/j.msec.2017.07.002 (2017)
Karunakaran, RG, Lu, C-H, Zhang, Z, Yang, S, “Highly Transparent Superhydrophobic Surfaces from the Coassembly of Nanoparticles (≤100 nm).” Langmuir, 27 (8) 4594–4602. https://doi.org/10.1021/la104067c (2011)
Zhang, M, Wang, P, Sun, H, Wang, Z, “Superhydrophobic Surface with Hierarchical Architecture and Bimetallic Composition for Enhanced Antibacterial Activity.” ACS Appl. Mater. Interfaces, 6 (24) 22108–22115. https://doi.org/10.1021/am505490w (2014)
Masyita, A, Mustika Sari, R, Dwi Astuti, A, Yasir, B, Rahma Rumata, N, Emran, TB, Nainu, F, Simal-Gandara, J, “Terpenes and Terpenoids as Main Bioactive Compounds of Essential Oils, Their Roles in Human Health and Potential Application as Natural Food Preservatives.” Food Chem. X, 13 100217. https://doi.org/10.1016/j.fochx.2022.100217 (2022)
Pandey, SK, Bhandari, S, Sarma, N, Begum, T, Munda, S, Baruah, J, Gogoi, R, Haldar, S, Lal, M, “Essential Oil Compositions, Pharmacological Importance and Agro Technological Practices of Patchouli (Pogostemon cablin Benth.): A Review.” J. Essential Oil Bear. Plants, 24 (6) 1212–1226. https://doi.org/10.1080/0972060X.2021.1995511 (2021)
Wińska, K, Mączka, W, Łyczko, J, Grabarczyk, M, Czubaszek, A, Szumny, A, “Essential Oils as Antimicrobial Agents—Myth or Real Alternative?” Molecules (Basel, Switzerland), 24 (11) 2130. https://doi.org/10.3390/molecules24112130 (2019)
Dhifi, W, Bellili, S, Jazi, S, Bahloul, N, Mnif, W, “Essential Oils’ Chemical Characterization and Investigation of Some Biological Activities: A Critical Review.” Medicines (Basel, Switzerland), 3 (4) 25. https://doi.org/10.3390/medicines3040025 (2016)
Mieres-Castro, D, Ahmar, S, Shabbir, R, Mora-Poblete, F, “Antiviral Activities of Eucalyptus Essential Oils: Their Effectiveness as Therapeutic Targets Against Human Viruses.” Pharmaceuticals, 14 (12) 1210. https://doi.org/10.3390/ph14121210 (2021)
Ait-Ouazzou, A, Cherrat, L, Espina, L, Lorán, S, Rota, C, Pagán, R, “The Antimicrobial Activity of Hydrophobic Essential Oil Constituents Acting Alone or in Combined Processes of Food Preservation.” Innov. Food Sci. Emerg. Technol., 12 (3) 320–329. https://doi.org/10.1016/j.ifset.2011.04.004 (2011)
Silva, FAB, Chagas-Silva, FA, Florenzano, FH, Pissetti, FL, “Poly(dimethylsiloxane) and Poly[vinyltrimethoxysilane-co-2-(dimethylamino) ethyl methacrylate] Based Cross-Linked Organic-Inorganic Hybrid Adsorbent for Copper(II) Removal from Aqueous Solutions.” J. Braz. Chem. Soc., 27 (12) 2181–2191. https://doi.org/10.5935/0103-5053.20160110 (2016)
Bardelli, T, Marano, C, Briatico Vangosa, F, “Polydimethylsiloxane Crosslinking Kinetics: A Systematic Study on Sylgard184 Comparing Rheological and Thermal Approaches.” J. Appl. Polym. Sci., 138 (39) 51013. https://doi.org/10.1002/app.51013 (2021)
Duffy, DC, Olivier, JCM, Schueller, JA, Whitesides, GM, “Rapid Prototyping of Microfluidic Systems in Poly(demethylsiloxane).” Anal. Chem., 70 4974–4984. https://doi.org/10.1021/ac980656z (1998)
Duffy, DC, Brittain, JA, Whitesides, GM, “Rapid Prototyping of Microfluidic Switches in Poly(dimethylsiloxane) and Their Actuation by Electro-Osmotic Flow.” J. Micromech. Microeng., 9 211–217. https://doi.org/10.1088/0960-1317/9/3/301 (1999)
Koh, KS, Chin, J, Chia, J, Chiang, CL, “Quantitative Studies on PDMS-PDMS Interface Bonding with Piranha Solution and Its Swelling Effect.” Micromachines, 3 427–441. https://doi.org/10.3390/mi3020427 (2012)
Kwak, Y, Kang, Y, Park, W, Jo, E, Kim, J, “Fabrication of Fine-Pored Polydimethylsiloxane Using an Isopropyl Alcohol and Water Mixture for Adjustable Mechanical, Optical, and Thermal Properties.” RSC Adv., 11 18061–18067. https://doi.org/10.1039/D1RA02466C (2021)
Hidouri, S, Jafari, R, Fournier, C, Girard, C, Momen, G, “Formulation of Nanohybrid Coating Based on Essential Oil and Fluoroalkyl Silane for Antibacterial Superhydrophobic Surfaces.” Appl. Surf. Sci. Adv., 9 100252. https://doi.org/10.1016/j.apsadv.2022.100252 (2022)
Johnson LM, Gao L, Shields CW, Smith M, Efimenko K, Cushing K, Genzer J, López GP. “Elastomeric Microparticles for Acoustic Mediated Bioseparations.” J. Nanobiotechnology, 11 22. https://doi.org/10.1186/1477-3155-11-22 (2013)
Wang, L, Fu, J, Jiang, X, Li, D, “Solid-Phase Extraction Based on PDMS/Ionic Liquid Sponge Followed by Gas Chromatography-Mass Spectrometry for Rapid and Sensitive Determination of Volatile Components in Lavender.” Phytochem. Anal., 34 (2) 225–239 (2023)
Miranda, I, Souza, A, Sousa, P, Ribeiro, J, Castanheira, EMS, Lima, R, Minas, G, “Properties and Applications of PDMS for Biomedical Engineering: A Review.” J. Funct. Biomater., 13 (1) 2. https://doi.org/10.3390/jfb13010002 (2022)
Ariati, R, Sales, F, Souza, A, Lima, RA, Ribeiro, J, “Polydimethylsiloxane Composites Characterization and Its Applications: A Review.” Polymers, 13 (23) 4258. https://doi.org/10.3390/polym13234258 (2021)
Yang, L, Hao, X, Wang, C, Zhang, B, Wang, W, “Rapid and Low Cost Replication of Complex Microfluidic Structures with PDMS Double Casting Technology.” Microsyst. Technol., 20 (10) 1933–1940. https://doi.org/10.1007/s00542-013-2004-8 (2014)
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The authors thank Dr. Catherine Gerard and Claire Fournier for their assistance with the antibacterial tests.
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Hidouri, S., Jafari, R. & Momen, G. Development of a polydimethylsiloxane–Eucalyptus essential oil antibacterial coating. J Coat Technol Res 21, 747–760 (2024). https://doi.org/10.1007/s11998-023-00854-8
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DOI: https://doi.org/10.1007/s11998-023-00854-8