Abstract
With the increasing awareness of environmental protection, the interest in the development of biodegradable materials has become increasingly popular. At present, wheat gluten protein (WGP), soybean protein isolate (SPI), and Zein have made some progress in the application of packaging materials. At the same time, beeswax (BW) is widely applied in the preparation of food coatings. In this study, composite films were prepared by adding BW to three different types of proteins. Various properties of the composite film, such as thickness, color, mechanical properties, and thermal stability, were tested. The thickness of the film was significantly increased after adding BW. Regarding the mechanical properties, the BW didn’t improve the composite film’s tensile strength. The results of the SEM indicated that the surface morphology of the composite films changed due to the interaction between BW and protein molecules. Besides, the addition of BW resulted in a decrease of thermal stability. The Td of the Zein film (77 ℃) and WGP film (106 ℃) were decreased to 72℃ and 98℃, respectively. The study shows that the composite films will have the opportunity to be applied in the food package field in the future and further replace the traditional petroleum-based films.
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Data Availability Statement
All data generated or analyzed during this study are included in this published article.
References
Adilah, Z. A. M., Jamilah, B., & Hanani, Z. A. N. (2018). Functional and antioxidant properties of protein-based films incorporated with mango kernel extract for active packaging. Food Hydrocolloids, 74, 207–218. https://doi.org/10.1016/j.foodhyd.2017.08.017
Alehosseini, A., Gómez-Mascaraque, L. G., Martínez-Sanz, M., & López-Rubio, A. (2019). Electrospun curcumin-loaded protein nanofiber mats as active/bioactive coatings for food packaging applications. Food Hydrocolloids, 87, 758–771. https://doi.org/10.1016/j.foodhyd.2018.08.056
Andreuccetti, C., Carvalho, R. A., Galicia-García, T., Martínez-Bustos, F., & Grosso, C. R. F. (2011). Effect of surfactants on the functional properties of gelatin-based edible films. Journal of Food Engineering, 103(2), 129–136. https://doi.org/10.1016/j.jfoodeng.2010.10.007
Basch, C., Jagus, R., & Flores, S. (2013). Physical and antimicrobial properties of tapioca starch-HPMC edible films incorporated with nisin and/or potassium sorbate. Food and Bioprocess Technology, 6(9), 2419–2428. https://doi.org/10.1007/s11947-012-0860-3
Bermúdez-Oria, A., Rodríguez-Gutiérrez, G., Rubio-Senent, F., Fernández-Prior, Á., & Fernández-Bolaños, J. (2018). Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage. Meat Science, 148, 213–218. https://doi.org/10.1016/j.meatsci.2018.07.003
Chao, Z., Ma, Y., Zhao, X., & Dan, M. (2010). Development of soybean protein-isolate edible films incorporated with beeswax, Span 20, and glycerol. Journal of Food Science, 75(6), C493–C497. https://doi.org/10.1111/j.1750-3841.2010.01666.x
Chen, C. H., & Lai, L. S. (2008). Mechanical and water vapor barrier properties of tapioca starch/decolorized hsian-tsao leaf gum films in the presence of plasticizer. Food Hydrocolloids, 22(8), 1584–1595. https://doi.org/10.1016/j.foodhyd.2007.11.006
Cheng, Y., Sun, C., Zhai, X., Zhang, R., & Hou, H. (2021). Effect of lipids with different physical state on the physicochemical properties of starch/gelatin edible films prepared by extrusion blowing. International Journal of Biological Macromolecules, 185, 1005–1014. https://doi.org/10.1016/j.ijbiomac.2021.06.203
Cheng, Y., Zhai, X. S., Wu, Y. H., Li, C., Zhang, R., Sun, C. C., Wang, W, T., & Hou, H, X. (2022). Effects of natural wax types on the physicochemical properties of starch/gelatin edible films fabricated by extrusion blowing. Food chemistry, 401, 134081. https://doi.org/10.1016/j.foodchem.2022.134081
Cruces, F., García, M. G., & Ochoa, N. A. (2021). Reduction of water vapor permeability in food multilayer biopackaging by epitaxial crystallization of beeswax. Food Bioprocess Technology, 14, 1244–1255. https://doi.org/10.1007/s11947-021-02628-9
De Vargas, V. H., Marczak, L. D. F., Flôres, S. H., & Mercaliet, G. D. (2022). Advanced technologies applied to enhance properties and structure of films and coatings: A review. Food Bioprocess Technology, 15, 1224–1247. https://doi.org/10.1007/s11947-022-02768-6
Erdem, B. G., Dıblan, S., & Kaya, S. (2019). Development and structural assessment of whey protein isolate/sunflower seed oil biocomposite film. Food and Bioproducts Processing, 118, 270–280. https://doi.org/10.1016/j.fbp.2019.09.015
Erdem, B. G., Dıblan, S., & Kaya, S. (2021). A comprehensive study on sorption, water barrier, and physicochemical properties of some protein- and carbohydrate-based edible films. Food Bioprocess Technology, 14, 2161–2179. https://doi.org/10.1007/s11947-021-02712-0
Fabra, M. J., Talens, P., & Chiralt, A. (2008). Tensile properties and water vapor permeability of sodium caseinate films containing oleic acid–beeswax mixtures. Journal of Food Engineering, 85(3), 393–400. https://doi.org/10.1016/j.jfoodeng.2007.07.022
Fabra, M. J., Talens, P., & Chiralt, A. (2009). Microstructure and optical properties of sodium caseinate films containing oleic acid–beeswax mixtures. Food Hydrocolloids, 23(3), 676–683. https://doi.org/10.1016/j.foodhyd.2008.04.015
Ghanbarzadeh, B., Musavi, M., Oromiehie, A. R., Rezayi, K., Rad, E. R., & Milani, J. (2007). Effect of plasticizing sugars on water vapor permeability, surface energy and microstructure properties of zein films. LWT - Food Science and Technology, 40(7), 1191–1197. https://doi.org/10.1016/j.lwt.2006.07.008
Gontard, N., Guilbert, S., & Cuq, J. L. (2010). Edible wheat gluten films: Influence of the main process variables on film properties using response surface methodology. Journal of Food Science, 57(1), 190–195. https://doi.org/10.1111/j.1365-2621.1992.tb05453.x
Guo, G., Zhang, C., Du, Z., Zou, W., Tian, H., Xiang, A., & Li, H. (2015). Structure and property of biodegradable soy protein isolate/PBAT blends. Industrial Crops & Products, 74, 731–736. https://doi.org/10.1016/j.indcrop.2015.06.009
He, C., Hu, Y., Wang, Y., Liao, Y., Xiong, H., Selomulya, C., et al. (2020). Complete waste recycling strategies for improving the accessibility of rice protein films. Green Chemistry, 22(2), 490–503. https://doi.org/10.1039/C9GC03354H
Jiang, L., Jia, F., Han, Y., Meng, X., Xiao, Y., & Bai, S. (2021). Development and characterization of zein edible films incorporated with catechin/β-cyclodextrin inclusion complex nanoparticles. Carbohydrate Polymers, 261, 117877. https://doi.org/10.1016/j.carbpol.2021.117877
Jie, H. C., Tay, W., Ong, D., Liebl, D., & Henry, C. J. (2021). Physicochemical, textural and structural characteristics of wheat gluten-soy protein composited meat analogues prepared with the mechanical elongation method. Food Structure, 28(2), 100183. https://doi.org/10.1016/j.foostr.2021.100183
Jiménez, A., Fabra, M. J., Talens, P., & Chiralt, A. (2012). Edible and biodegradable starch films: A review. Food and Bioprocess Technology, 5(6), 2058–2076. https://doi.org/10.1007/s11947-012-0835-4
Kai, L., Xiangxin, X., Huiping, L., Zitian, L., Kaixuan, Z., & Yunhao, M., et al. (2021). Mechanical properties and water sensitivity of soybean protein isolate film improved by incorporation of sodium caseinate and transglutaminase. Progress in Organic Coatings, 153, 106154. https://doi.org/10.1016/j.porgcoat.2021.106154
Kang, H., Wang, Z., Zhang, W., Li, J., & Zhang, S. (2016). Physico-chemical properties improvement of soy protein isolate films through caffeic acid incorporation and tri-functional aziridine hybridization. Food Hydrocolloids, 61, 923–932. https://doi.org/10.1016/j.foodhyd.2016.07.009
Khanzadi, M., Jafari, S. M., Mirzaei, H., Chegini, F. K., & Dehnad, D. (2015). Physical and mechanical properties in biodegradable films of whey protein concentrate–pullulan by application of beeswax. Carbohydrate Polymers, 118, 24–29. https://doi.org/10.1016/j.carbpol.2014.11.015
Khashayary, S., & Aarabi, A. (2021). Evaluation of physico-mechanical and antifungal properties of gluten-based film incorporated with vanillin, salicylic acid, and montmorillonite (Cloisite 15A). Food Bioprocess Technology, 14, 665–678. https://doi.org/10.1007/s11947-021-02598-y
Kowalczyk, D., Kordowska-Wiater, M., Sołowiej, B., & Baraniak, B. (2015). Physicochemical and antimicrobial properties of biopolymer-candelilla wax emulsion films containing potassium sorbate – A comparative study. Food Bioprocess Technology, 8, 567–579. https://doi.org/10.1007/s11947-014-1423-6
Kumar, R., Praveen, R., Rani, S., Sharma, K., Tiwary, P. K., & DineshKumar, K. (2019). ZnSe Nanoparticles reinforced biopolymeric soy protein isolate film. Journal of Renewable Materials, 7(8), 749–761. https://doi.org/10.32604/jrm.2019.06286
Liao, L., Wang, Q., & Zhao, M. (2013). Functional, conformational and topographical changes of succinic acid deamidated wheat gluten upon freeze- and spray-drying: A comparative study. LWT - Food Science and Technology, 50(1), 177–184. https://doi.org/10.1016/j.lwt.2012.06.002
Li, X., Wei, Y., Jiang, S., Zhou, Y., & Li, J. (2021). Full bio-based soy protein isolate film enhanced by chicken feather keratin. Macromolecular Materials and Engineering, 306(5), 2100004. https://doi.org/10.1002/mame.202100004
Li, Y., Bai, Y., Huang, J., Yuan, C., & Hu, Y. (2019). Airglow discharge plasma treatment affects the surface structure and physical properties of zein films. Journal of Food Engineering, 273, 109813. https://doi.org/10.1016/j.jfoodeng.2019.109813
Liu, J., Meng, G., Liu, S., Kan, J., & Jin, C. (2017). Preparation and characterization of protocatechuic acid grafted chitosan films with antioxidant activity. Food Hydrocolloids, 63, 457–466. https://doi.org/10.1016/j.foodhyd.2016.09.035
Ma, Y., Bai, H., Jia, X., Lu, Y., Cui, H., & Guo, X. (2012). Factors affecting the physical properties of edible composite film prepared from zein and wheat gluten. Molecules, 17(4), 3794–3804. https://doi.org/10.3390/molecules17043794
Manassero, C. A., Beaumal, V., Vaudagna, S. R., Speroni, F., & Anton, M. (2018). Calcium addition, ph and high hydrostatic pressure effects on soybean protein isolates—part 2: Emulsifying properties. Food Bioprocess Technology, 11, 2079–2093. https://doi.org/10.1007/s11947-018-2164-8
Mandal, A., & Chakrabarty, D. (2015). Characterization of nanocellulose reinforced semi-interpenetrating polymer network of poly (vinyl alcohol) & polyacrylamide composite films. Carbohydrate Polymers, 134(10), 240–250. https://doi.org/10.1016/j.carbpol.2015.07.093
Meng, R., Wu, Z., Xie, Q. -T., Cheng, J. -S., & Zhang, B. (2021). Preparation and characterization of zein/carboxymethyl dextrin nanoparticles to encapsulate curcumin: Physicochemical stability, antioxidant activity and controlled release properties. Food Chemistry, 340, 127893. https://doi.org/10.1016/j.foodchem.2020.127893
Monedero, F. M., Fabra, M. J., Talens, P., & Chiralt, A. (2009). Effect of oleic acid–beeswax mixtures on mechanical, optical and water barrier properties of soy protein isolate based films. Journal of Food Engineering, 91(4), 509–515. https://doi.org/10.1016/j.jfoodeng.2008.09.034
Nogueira, D., & Martins, V. G. (2019). Use of different proteins to produce biodegradable films and blends. Journal of Polymers and the Environment, 27(9), 2027–2039. https://doi.org/10.1007/s10924-019-01494-z
Ochoa, T. A., Almendárez, B. E. G., Reyes, A. A., Pastrana, D. M. R., Gutiérrez López, G. F. G., Belloso, O. M., & González, C. R. (2017). Design and characterization of corn starch edible films including beeswax and natural antimicrobials. Food Bioprocess Technology, 10, 103–114. https://doi.org/10.1007/s11947-016-1800-4
Oh, M., Ma, Q., Simsek, S., Bajwa, D., & Jiang, L. (2019). Comparative study of zein- and gluten-based wood adhesives containing cellulose nanofibers and crosslinking agent for improved bond strength. International Journal of Adhesion and Adhesives, 92, 44–57. https://doi.org/10.1016/j.ijadhadh.2019.04.004
Omar-Aziz, M., Khodaiyan, F., Yarmand, M. S., Mousavi, M., & Hosseini, S. S. (2020). Combined effects of octenylsuccination and beeswax on pullulan films: Water-resistant and mechanical properties. Carbohydrate Polymers, 255(6), 117471. https://doi.org/10.1016/j.carbpol.2020.117471
Pei, X., Jiao, H., Fu, H., Yin, X., & Zhang, L. (2020). Facile construction of a highly dispersed Pt nanocatalyst anchored on biomass-derived N/O-doped carbon nanofibrous microspheres and its catalytic hydrogenation. ACS Applied Materials & Interfaces, 12(46), 51459–51467. https://doi.org/10.1021/acsami.0c14581
Rani, S., & Kumar, R. (2019). A review on material and antimicrobial properties of soy protein isolate film. Journal of Polymers and the Environment, 27(8), 1613–1628. https://doi.org/10.1007/s10924-019-01456-5
Rocca-Smith, J. R., Marcuzzo, E., Karbowiak, T., Centa, J., Giacometti, M., Scapin, F., Venir, E., Sensidoni, A., & Debeaufort, F. (2016). Effect of lipid incorporation on functional properties of wheat gluten based edible films. Journal of Cereal Science, 69, 275–282. https://doi.org/10.1016/j.jcs.2016.04.001
Rovera, C., Türe, H., Hedenqvist, M. S., & Farris, S. (2020). Water vapor barrier properties of wheat gluten/silica hybrid coatings on paperboard for food packaging applications. Food Packaging and Shelf Life, 26, 100561. https://doi.org/10.1016/j.fpsl.2020.100561
Shaikh, M., Haider, S., Ali, T. M., & Hasnain, A. (2019). Physical thermal mechanical and barrier properties of pearl millet starch films as affected by levels of acetylation and hydroxypropylation. International Journal of Biological Macromolecules, 124, 209–219. https://doi.org/10.1016/j.ijbiomac.2018.11.135
Suwannasang, S., Thumthanaruk, B., Zhong, Q., Uttapap, D., Puttanlek, C., Vatanyoopaisarn, S., & Rungsardthong, V. (2021). The improved properties of zein encapsulating and stabilizing sacha inchi oil by surfactant combination of lecithin and Tween 80. Food Bioprocess Technology, 14, 2078–2090. https://doi.org/10.1007/s11947-021-02706-y
Talens, P., & Krochta, J. M. (2010). Plasticizing effects of beeswax and carnauba wax on tensile and water vapor permeability properties of whey protein films. Journal of Food Science, 70(3), 239–243. https://doi.org/10.1111/j.1365-2621.2005.tb07141.x
Tanada-Palmu, P. S., & Grosso, C. (2005). Effect of edible wheat gluten-based films and coatings on refrigerated strawberry (Fragaria ananassa) quality. Postharvest Biology and Technology, 36(2), 199–208. https://doi.org/10.1016/j.postharvbio.2004.12.003
Tang, S., Wang, Z., Li, W., Li, M., Deng, Q., Wang, Y., Li, C., & Chu, P. K. (2019). Ecofriendly and biodegradable soybean protein isolate films incorporated with ZnO nanoparticles for food packaging. ACS Applied Bio Materials, 2(5), 2202–2207. https://doi.org/10.1021/acsabm.9b00170
Tavares, L., Souza, H., Gonalves, M. P., & Rocha, C. (2020). Physicochemical and microstructural properties of composite edible film obtained by complex coacervation between chitosan and whey protein isolate. Food Hydrocolloids, 113, 106471. https://doi.org/10.1016/j.foodhyd.2020.106471
Thomas, S., Jimenez, A., Torre, L., Chiralt, P. D., & Gopakumar. (2016). Revalorization of sunflower stalks as novel sources of cellulose nanofibrils and nanocrystals and their effect on wheat gluten bionanocomposite properties. Carbohydrate Polymers, 149, 357–368. https://doi.org/10.1016/j.carbpol.2016.04.120
Vogler, E. A. (1998). Structure and reactivity of water at biomaterial surfaces. Advances in Colloid and Interface Science, 74, 69–117. https://doi.org/10.1016/s0001-8686(97)00040-7
Wang, Z., Zhang, N., Wang, H. Y., Sui, S. Y., & Ma, Z. S. (2014a). The effects of ultrasonic/microwave assisted treatment on the properties of soy protein isolate/titanium dioxide films. LWT- Food Science and Technology, 57(2), 548–555. https://doi.org/10.1016/j.lwt.2014.01.036
Wang, Z., Zhou, J., Wang, X. -X., Zhang, N., Sun, X. -X., & Ma, Z. -S. (2014b). The effects of ultrasonic/microwave assisted treatment on the water vapor barrier properties of soybean protein isolate-based oleic acid/stearic acid blend edible films. Food Hydrocolloids, 35, 51–58. https://doi.org/10.1016/j.foodhyd.2013.07.006
Woods, K. K., & Selling, G. W. (2010). Melt reaction of zein with glyoxal to improve tensile strength and reduce solubility. Journal of Applied Polymer Science, 109(4), 2375–2383. https://doi.org/10.1002/app.28334
Zhang, C., Ma, Y., Guo, K., & Zhao, X. (2012). High-pressure homogenization lowers water vapor permeability of soybean protein isolate–beeswax films. Journal of Agricultural and Food Chemistry, 60(9), 2219–2223. https://doi.org/10.1021/jf2035109
Zhan, F., Yan, X., Sheng, F., & Li, B. (2020). Facile in situ synthesis of silver nanoparticles on tannic acid/zein electrospun membranes and their antibacterial, catalytic and antioxidant activities. Food Chemistry, 330, 127172. https://doi.org/10.1016/j.foodchem.2020.127172
Zhang, M., Zhang, L., Li, M., & Sun, Q. (2021). Inhibitory effects of sorbitol on the collapse and deterioration of gluten network in fresh noodles during storage. Food Chemistry, 344, 128638. https://doi.org/10.1016/j.foodchem.2020.128638
Zhang, Y., Zhang, Y., Cao, Q., Wang, C., Yang, C., Li, Y., & Zhou, J. (2020). Novel porous oil-water separation material with super-hydrophobicity and super-oleophilicity prepared from beeswax, lignin, and cotton. Science of The Total Environment, 706, 135807. https://doi.org/10.1016/j.scitotenv.2019.13580
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This work was supported by the National Natural Science Foundation of China (No. 32172205).
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Jiangkai Duan: conceptualization, methodology, validation, formal analysis, investigation, data curation, writing—original draft. Qin Zhou: methodology, investigation, writing—review, software and editing. Menghan Fu: conceptualization, methodology, writing—review and editing. Mengyuan Cao: methodology, writing—review and software. Miao Jiang: formal analysis, writing—review and editing. Liqiang Zhang: writing—review and editing. Xiang Duan: methodology, formal analysis, funding acquisition, writing—review and editing.
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Duan, J., Zhou, Q., Fu, M. et al. Research on Properties of Edible Films Prepared from Zein, Soy Protein Isolate, Wheat Gluten Protein by Adding Beeswax. Food Bioprocess Technol 16, 2443–2454 (2023). https://doi.org/10.1007/s11947-023-03077-2
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DOI: https://doi.org/10.1007/s11947-023-03077-2