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Separation and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase Systems Based on Triblock Thermosensitive Copolymers

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Abstract

C-phycocyanin is a high market value compound derived from cyanobacterium Spirulina platensis and has a wide range of applications in pharmaceutical and food industries as a useful biochemically active compound. In this study, essentially an aqueous two-phase system (ATPS) is presented for purification of C-phycocyanin. The phase behavior of ATPSs that include Pluronic copolymers and salts was evaluated systematically. After that, the effects of different parameters such as salt type, copolymer structure, pH of solution, tie line length (TLL), system’s temperature, and volume ratio for purification of C-phycocyanin were investigated. The results revealed that Pluronic 10R5/potassium phosphate buffer system is the most appropriate system for promoting the C-phycocyanin separation from other contaminants. Besides, the purity index of C-phycocyanin was enhanced by up to 3.92 at TLL of 46.31%, pH = 6, volume ratio of 0.34, and temperature of 35 °C without any loss of stability. At these conditions, the obtained C-phycocyanin recovery was 90%. After that, the purity factor reached to 5.9 by applying the ultrafiltration method. In addition, the results of circular dichroism (CD) spectroscopy proved that the C-phycocyanin structure remained intact during the purification step. Finally, 78% of 10R5 copolymer was recovered by increasing the temperature above 57 °C (micelle formation (which can be used in new ATPS.

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Funding

The study was financially supported by the Iran National Science Foundation (INSF) with grant number 98020043.

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Authors and Affiliations

  1. Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

    Alireza Ebrahimi, Gholamreza Pazuki, Mehrdad Mozaffarian & Farzaneh Ghazizadeh Ahsaie

  2. Mechanical Engineering Department, KU Leuven, 3000, Louvain, Belgium

    Hamed Abedini

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  1. Alireza Ebrahimi
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  2. Gholamreza Pazuki
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Contributions

Alireza Ebrahimi: conceptualization, methodology, investigation, formal analysis, writing–original draft. Gholamreza Pazuki: investigation, writing–review and editing, supervision. Mehrdad Mozaffarian: writing–review and editing, supervision. Farzaneh Ghazizadeh Ahsaie: methodology, investigation, writing–review and editing. Hamed Abedini: investigation, supervision.

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Ebrahimi, A., Pazuki, G., Mozaffarian, M. et al. Separation and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase Systems Based on Triblock Thermosensitive Copolymers. Food Bioprocess Technol 16, 2582–2597 (2023). https://doi.org/10.1007/s11947-023-03057-6

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