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Sequential Microwave-Ultrasound-Assisted Extraction for Isolation of Piperine from Black Pepper (Piper nigrum L.)

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Abstract

Piperine is the natural bioactive component of black pepper (Piper nigrum L.) with several astounding therapeutic properties. In this study, sequential microwave-ultrasound-assisted extraction approach was used for isolation of piperine from black pepper. The effect of various factors such as extraction solvent, particle size of pepper, solvent to solid ratio, microwave power and time and ultrasound temperature and time on the extraction yield of piperine was considered. The maximum extraction yield was 46.6 mg piperine/g pepper which was obtained using ethanol as solvent at the particle size of 0.15 mm, solvent to solid ratio of 20:1, microwave power of 100 W for 1 min, and ultrasound temperature of 50 ° C for 30 min. This extraction yield was higher than those obtained by Soxhlet (39.1 mg/g), microwave-assisted (38.8 mg/g) and ultrasound-assisted (37.0 mg/g) extractions. The purity of the extracted piperine was 81.4% as determined by HPLC analysis. The FTIR and UV-vis analyses confirmed that the structure of piperine remained intact after extraction and purification which is very important for medicinal applications.

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References

  • Agarwal, O. (2006). Natural products (Vol. 2) Krishna Prakashan Media.

    Google Scholar 

  • Barringer, S. A., Davis, E. A., Gordon, J., Ayappa, K. G., & Davis, H. (1995). Microwave-heating temperature profiles for thin slabs compared to maxwell and lambert law predictions. Journal of Food Science, 60(5), 1137–1142.

    Article  CAS  Google Scholar 

  • Camel, V. (2000). Microwave-assisted solvent extraction of environmental samples. TrAC Trends in Analytical Chemistry, 19(4), 229–248.

    Article  CAS  Google Scholar 

  • Cao, X., Ye, X., Lu, Y., Yu, Y., & Mo, W. (2009). Ionic liquid-based ultrasonic-assisted extraction of piperine from white pepper. Analytica Chimica Acta, 640(1), 47–51.

    Article  CAS  Google Scholar 

  • Castro-López, C., Ventura-Sobrevilla, J. M., González-Hernández, M. D., Rojas, R., Ascacio-Valdés, J. A., Aguilar, C. N. & Martínez-Ávila, G. C. (2017). Impact of extraction techniques on antioxidant capacities and phytochemical composition of polyphenol-rich extracts. Food Chemistry. In press.

  • Chan, C.-H., Yusoff, R., Ngoh, G.-C., & Kung, F. W.-L. (2011). Microwave-assisted extractions of active ingredients from plants. Journal of Chromatography A, 1218(37), 6213–6225.

    Article  CAS  Google Scholar 

  • Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560.

    Article  CAS  Google Scholar 

  • Cvetanović, A., Švarc-Gajić, J., Zeković, Z., Mašković, P., Đurović, S., Zengin, G., Delerue-Matos, C., Lozano-Sánchez, J., & Jakišić, A. (2017). Chemical and biological insights on aronia stems extracts obtained by different extraction techniques: From wastes to functional products. Journal of Supercritical Fluids, 128, 173–181.

    Article  Google Scholar 

  • Duarte, S. H., dos Santos, P., Michelon, M., de Pinho Oliveira, S. M., Martínez, J. & Maugeri, F. (2017). Recovery of yeast lipids using different cell disruption techniques and supercritical CO2 extraction. Biochemical Engineering Journal, In press.

  • Dutta, S., & Bhattacharjee, P. (2015). Enzyme-assisted supercritical carbon dioxide extraction of black pepper oleoresin for enhanced yield of piperine-rich extract. Journal of Bioscience and Bioengineering, 120(1), 17–23.

    Article  CAS  Google Scholar 

  • Entezari, M. H., & Kruus, P. (1996). Effect of frequency on sonochemical reactions II. Temperature and intensity effects. Ultrasonics Sonochemistry, 3(1), 19–24.

    Article  CAS  Google Scholar 

  • Eskilsson, C. S., & Björklund, E. (2000). Analytical-scale microwave-assisted extraction. Journal of Chromatography A, 902(1), 227–250.

    Article  CAS  Google Scholar 

  • Gaikar, V. G. & Raman, G. (2002) Process for extraction of piperine from piper species. Google Patents.

    Google Scholar 

  • Gorgani, L., Mohammadi, M., Najafpour, G. D., & Nikzad, M. (2017). Piperine—the bioactive compound of black pepper: from isolation to medicinal formulations. Comprehensive Reviews in Food Science and Food Safety, 16(1), 124–140.

    Article  CAS  Google Scholar 

  • Holzwarth, A., Lou, J., Hatton, T. A., & Laibinis, P. E. (1998). Enhanced microwave heating of nonpolar solvents by dispersed magnetic nanoparticles. Industrial & Engineering Chemistry Research, 37(7), 2701–2706.

    Article  CAS  Google Scholar 

  • Kala, H. K., Mehta, R., Sen, K. K., Tandey, R., & Mandal, V. (2017). Strategizing method optimization of microwave-assisted extraction of plant phenolics by developing standard working principles for universal robust optimization. Analytical Methods, 9(13), 2089–2103.

    Article  CAS  Google Scholar 

  • Lahijani, P., Zainal, Z. A., Mohamed, A. R., & Mohammadi, M. (2014). Microwave-enhanced CO2 gasification of oil palm shell char. Bioresource Technology, 158, 193–200.

    Article  CAS  Google Scholar 

  • Larhed, M., & Olofsson, K. (2006). Microwave methods in organic synthesis, vol. 266. Berlin Heidelberg: Springer.

  • Li, H., Chen, B., Nie, L., & Yao, S. (2004). Solvent effects on focused microwave assisted extraction of polyphenolic acids from Eucommia ulmodies. Phytochemical Analysis, 15(5), 306–312.

    Article  CAS  Google Scholar 

  • Lide, D. R. (2012). CRC handbook of chemistry and physics. 12J204 (92nd ed.). Boca Raton: CRC Press, Florida.

    Google Scholar 

  • Mason, T. J., Chemat, F., & Vinatoru, M. (2011). The extraction of natural products using ultrasound or microwaves. Current Organic Chemistry, 15(2), 237–247.

    Article  CAS  Google Scholar 

  • Meghwal, M., & Goswami, T. (2013). Piper nigrum and piperine: an update. Phytotherapy Research, 27(8), 1121–1130.

    Article  CAS  Google Scholar 

  • Mishra, S. P., & Gaikar, V. G. (2009). Hydrotropic extraction process for recovery of forskolin from Coleus forskohlii roots. Industrial & Engineering Chemistry Research, 48(17), 8083–8090.

    Article  CAS  Google Scholar 

  • Osorio-Tobón, J. F., Carvalho, P. I., Rostagno, M. A., Petenate, A. J., & Meireles, M. A. A. (2014). Extraction of curcuminoids from deflavored turmeric (Curcuma longa L.) using pressurized liquids: process integration and economic evaluation. The Journal of Supercritical Fluids, 95, 167–174.

    Article  Google Scholar 

  • Pachauri, M., Gupta, E. D., & Ghosh, P. C. (2015). Piperine loaded PEG-PLGA nanoparticles: Preparation, characterization and targeted delivery for adjuvant breast cancer chemotherapy. Journal of Drug Delivery Science and Technology, 29, 269–282.

    Article  CAS  Google Scholar 

  • Parthasarathy, V. A., Chempakam, B., & Zachariah, T. J. (2008). Chemistry of spices. Calicut: CABI.

  • Peter, K. V. (2006). Handbook of herbs and spices, vol. 3. Cambridge: Woodhead Publishing.

  • Raman, G., & Gaikar, V. G. (2002a). Extraction of piperine from Piper nigrum (black pepper) by hydrotropic solubilization. Industrial & Engineering Chemistry Research, 41(12), 2966–2976.

    Article  CAS  Google Scholar 

  • Raman, G., & Gaikar, V. G. (2002b). Microwave-assisted extraction of piperine from Piper nigrum. Industrial & Engineering Chemistry Research, 41(10), 2521–2528.

    Article  CAS  Google Scholar 

  • Raso, J., Manas, P., Pagan, R., & Sala, F. J. (1999). Influence of different factors on the output power transferred into medium by ultrasound. Ultrasonics Sonochemistry, 5(4), 157–162.

    Article  CAS  Google Scholar 

  • Rathod, S. S., & Rathod, V. K. (2014). Extraction of piperine from Piper longum using ultrasound. Industrial Crops and Products, 58, 259–264.

    Article  CAS  Google Scholar 

  • Ravindran, P. (2003). Black pepper: Piper nigrum. Boca Raton: CRC Press, Florida.

    Google Scholar 

  • Roriz, C. L., Barros, L., Prieto, M., Barreiro, M. F., Morales, P., & Ferreira, I. C. (2017). Modern extraction techniques optimized to extract betacyanins from Gomphrena globosa L. Industrial Crops and Products, 105, 29–40.

    Article  CAS  Google Scholar 

  • Rostagno, M. A., & Prado, J. M. (2013). Natural product extraction: principles and applications, vol. 21. Cambridge: Royal Society of Chemistry.

  • Schulz, H., & Baranska, M. (2007). Identification and quantification of valuable plant substances by IR and Raman spectroscopy. Vibrational Spectroscopy, 43(1), 13–25.

    Article  CAS  Google Scholar 

  • Schulz, H., Baranska, M., Quilitzsch, R., Schütze, W., & Lösing, G. (2005). Characterization of peppercorn, pepper oil, and pepper oleoresin by vibrational spectroscopy methods. Journal of Agricultural and Food Chemistry, 53(9), 3358–3363.

    Article  CAS  Google Scholar 

  • Sharma, S., & Sahu, A. N. (2016). Development, characterization, and evaluation of hepatoprotective effect of Abutilon indicum and Piper longum Phytosomes. Pharmacognosy research, 8(1), 29.

    Article  CAS  Google Scholar 

  • Veerareddy, P., Vobalaboina, V., & Nahid, A. (2004). Formulation and evaluation of oil-in-water emulsions of piperine in visceral leishmaniasis. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 59(3), 194–197.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Biotechnology Research Laboratory, Department of Chemical Engineering, Babol Noshirvani University of Technology, Shariati Ave., Babol, 47148-71167, Iran

    Leila Gorgani, Maedeh Mohammadi, Ghasem D. Najafpour & Maryam Nikzad

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  1. Leila Gorgani
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  2. Maedeh Mohammadi
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  3. Ghasem D. Najafpour
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  4. Maryam Nikzad
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Correspondence to Maedeh Mohammadi.

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Gorgani, L., Mohammadi, M., Najafpour, G.D. et al. Sequential Microwave-Ultrasound-Assisted Extraction for Isolation of Piperine from Black Pepper (Piper nigrum L.). Food Bioprocess Technol 10, 2199–2207 (2017). https://doi.org/10.1007/s11947-017-1994-0

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  • DOI: https://doi.org/10.1007/s11947-017-1994-0

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