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Preparation and Release Behavior of Gelatin-Based Capsules of Antioxidants from Ethanolic Extracts of Thai Riceberry Bran

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Abstract

This study aimed to devise an environment-friendly and effective, yet simple and practicable, antioxidant extraction and encapsulation method from Riceberry bran, whose extract was used thereafter for developing highly efficient antioxidant capsules. Ethanolic Riceberry bran extracts with high total phenolic content, total flavonoid content, total anthocyanin content, and antioxidant activity (using DPPH radical scavenging activity and ferric reducing antioxidant power assay) were obtained using ultrasonic-assisted extraction. The optimum conditions for producing the capsules, such as types of gelatin, concentrations of gelatin, and the Riceberry bran extract concentrations, were studied. Capsules produced by incorporating 1% (w/v) of acid-treated gelatin (type A) and 1% (w/v) of Riceberry bran extract yielded higher chemical properties. When dispersed in water at 37 °C, the capsules exhibited a high release of antioxidants. Moreover, the capsule showed a lower degradation rate of antioxidants under simulated gastrointestinal conditions compared to the crude extract.

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References

  • Aewsiri, T., Benjakul, S., Visessanguan, W., Eun, J.-B., Wierenga, P. A., & Gruppen, H. (2009). Antioxidative activity and emulsifying properties of cuttlefish skin gelatin modified by oxidised phenolic compounds. Food Chemistry, 117(1), 160–168.

    Article  CAS  Google Scholar 

  • Arnao, M. B. (2000). Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends in Food Science & Technology, 11(11), 419–421.

    Article  CAS  Google Scholar 

  • Barnes, C. A., Elsaesser, A., Arkusz, J., Smok, A., Palus, J., Lesniak, A., et al. (2008). Reproducible comet assay of amorphous silica nanoparticles detects no genotoxicity. Nano Letters, 8(9), 3069–3074.

    Article  CAS  Google Scholar 

  • Barnes, J. S., Nguyen, H. P., Shen, S., & Schug, K. A. (2009). General method for extraction of blueberry anthocyanins and identification using high performance liquid chromatography–electrospray ionization-ion trap-time of flight-mass spectrometry. Journal of Chromatography A, 1216(23), 4728–4735.

    Article  CAS  Google Scholar 

  • Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70–76.

    Article  CAS  Google Scholar 

  • Bermúdez-Soto, M.-J., Tomás-Barberán, F.-A., & García-Conesa, M.-T. (2007). Stability of polyphenols in chokeberry (Aronia melanocarpa) subjected to in vitro gastric and pancreatic digestion. Food Chemistry, 102(3), 865–874.

    Article  Google Scholar 

  • Bouayed, J., Deußer, H., Hoffmann, L., & Bohn, T. (2012). Bioaccessible and dialysable polyphenols in selected apple varieties following in vitro digestion vs. their native patterns. Food Chemistry, 131(4), 1466–1472.

    Article  CAS  Google Scholar 

  • Çelik, S. E., Özyürek, M., Güçlü, K., & Apak, R. (2010). Solvent effects on the antioxidant capacity of lipophilic and hydrophilic antioxidants measured by CUPRAC, ABTS/persulphate and FRAP methods. Talanta, 81(4–5), 1300–1309.

    Article  Google Scholar 

  • Chemat, F., Tomao, V., & Virot, M. (2008). Ultrasound-assisted extraction in food analysis. In Handbook of food analysis instruments (pp. 85–103). Boca Raton: CRC press.

    Google Scholar 

  • Chotimarkorn, C., Benjakul, S., & Silalai, N. (2008). Antioxidant components and properties of five long-grained rice bran extracts from commercial available cultivars in Thailand. Food Chemistry, 111(3), 636–641.

    Article  CAS  Google Scholar 

  • Daiponmak, W., Senakun, C., & Siriamornpun, S. (2014). Antiglycation capacity and antioxidant activities of different pigmented Thai rice. International Journal of Food Science and Technology, 49(8), 1805–1810.

    Article  CAS  Google Scholar 

  • Deepa, N., Kaur, C., Singh, B., & Kapoor, H. (2006). Antioxidant activity in some red sweet pepper cultivars. Journal of Food Composition and Analysis, 19(6), 572–578.

    Article  CAS  Google Scholar 

  • Desai, K. G. H., & Jin Park, H. (2005). Recent developments in microencapsulation of food ingredients. Drying Technology, 23(7), 1361–1394.

    Article  CAS  Google Scholar 

  • Devi, N., & Maji, T. K. (2010). Genipin crosslinked microcapsules of gelatin A and κ-carrageenan polyelectrolyte complex for encapsulation of Neem (Azadirachta indica A. Juss.) seed oil. Polymer Bulletin, 65(4), 347–362.

    Article  CAS  Google Scholar 

  • Donini, C., Robinson, D., Colombo, P., Giordano, F., & Peppas, N. (2002). Preparation of poly (methacrylic acid-g-poly (ethylene glycol)) nanospheres from methacrylic monomers for pharmaceutical applications. International Journal of Pharmaceutics, 245(1), 83–91.

    Article  CAS  Google Scholar 

  • Elless, M. P., Blaylock, M. J., Huang, J. W., & Gussman, C. D. (2000). Plants as a natural source of concentrated mineral nutritional supplements. Food Chemistry, 71(2), 181–188.

    Article  CAS  Google Scholar 

  • Francis, F. J., & Markakis, P. C. (1989). Food colorants: anthocyanins. Critical Reviews in Food Science and Nutrition, 28(4), 273–314.

    Article  CAS  Google Scholar 

  • Garcia-Castello, E. M., Rodriguez-Lopez, A. D., Mayor, L., Ballesteros, R., Conidi, C., & Cassano, A. (2015). Optimization of conventional and ultrasound assisted extraction of flavonoids from grapefruit (Citrus paradisi L.) solid wastes. LWT - Food Science and Technology, 64(2), 1114–1122.

    Article  CAS  Google Scholar 

  • Gawlik-Dziki, U., Dziki, D., Baraniak, B., & Lin, R. (2009). The effect of simulated digestion in vitro on bioactivity of wheat bread with Tartary buckwheat flavones addition. LWT-Food Science and Technology, 42(1), 137–143.

    Article  CAS  Google Scholar 

  • Gharsallaoui, A., Roudaut, G., Chambin, O., Voilley, A., & Saurel, R. (2007). Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Research International, 40(9), 1107–1121.

    Article  CAS  Google Scholar 

  • Girard, M., Turgeon, S. L., & Gauthier, S. F. (2002). Interbiopolymer complexing between β-lactoglobulin and low- and high-methylated pectin measured by potentiometric titration and ultrafiltration. Food Hydrocolloids, 16(6), 585–591.

    Article  CAS  Google Scholar 

  • Giusti, M. M., & Wrolstad, R. E. (2005). Unit F1.2: characterization and measurement of anthocyanins by UV–visible spectroscopy. In R. E. Wrolstad (Ed.), Handbook of analytical food chemistry (pp. 19–31). New York: Wiley.

    Google Scholar 

  • Hudson, E. A., Dinh, P. A., Kokubun, T., Simmonds, M. S., & Gescher, A. (2000). Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiology Biomarkers and Prevention, 9(11), 1163–1170.

    CAS  Google Scholar 

  • Iqbal, S., Bhanger, M., & Anwar, F. (2005). Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chemistry, 93(2), 265–272.

    Article  CAS  Google Scholar 

  • Jariwalla, R. (2001). Rice-bran products: phytonutrients with potential applications in preventive and clinical medicine. Drugs under Experimental and Clinical Research, 27(1), 17–26.

    CAS  Google Scholar 

  • Laleh, G., Frydoonfar, H., Heidary, R., Jameei, R., & Zare, S. (2006). The effect of light, temperature, pH and species on stability of anthocyanin pigments in four Berberis species. Pakistan Journal of Nutrition, 5(1), 90–92.

    Article  Google Scholar 

  • Leardkamolkarn, V., Thongthep, W., Suttiarporn, P., Kongkachuichai, R., Wongpornchai, S., & Wanavijitr, A. (2011). Chemopreventive properties of the bran extracted from a newly-developed Thai rice: the Riceberry. Food Chemistry, 125(3), 978–985.

    Article  CAS  Google Scholar 

  • Leo, E., Forni, F., & Bernabei, M. T. (2000). Surface drug removal from ibuprofen-loaded PLA microspheres. International Journal of Pharmaceutics, 196(1), 1–9.

    Article  CAS  Google Scholar 

  • Liang, L., Wu, X., Zhao, T., Zhao, J., Li, F., Zou, Y., et al. (2012). In vitro bioaccessibility and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.) following simulated gastro-intestinal digestion. Food Research International, 46(1), 76–82.

    Article  CAS  Google Scholar 

  • Luzzi, L. A. (1970). Microencapsulation. Journal of Pharmaceutical Sciences, 59(10), 1367–1376.

    Article  CAS  Google Scholar 

  • Maji, T., Baruah, I., Dube, S., & Hussain, M. (2007). Microencapsulation of Zanthoxylum limonella oil (ZLO) in glutaraldehyde crosslinked gelatin for mosquito repellent application. Bioresource Technology, 98(4), 840–844.

    Article  CAS  Google Scholar 

  • Mamidipally, P. K., & Liu, S. X. (2004). First approach on rice bran oil extraction using limonene. European Journal of Lipid Science and Technology, 106(2), 122–125.

    Article  CAS  Google Scholar 

  • Mazza, G., & Miniati, E. (1993). Anthocyanins in fruits, vegetables, and grains. London: CRC press.

    Google Scholar 

  • Min, B., Gu, L., McClung, A. M., Bergman, C. J., & Chen, M.-H. (2012). Free and bound total phenolic concentrations, antioxidant capacities, and profiles of proanthocyanidins and anthocyanins in whole grain rice (Oryza sativa L.) of different bran colours. Food Chemistry, 133(3), 715–722.

    Article  CAS  Google Scholar 

  • Müller, R., Radtke, M., & Wissing, S. (2002). Nanostructured lipid matrices for improved microencapsulation of drugs. International Journal of Pharmaceutics, 242(1), 121–128.

    Article  Google Scholar 

  • Ozgen, M., Reese, R. N., Tulio, A. Z., Scheerens, J. C., & Miller, A. R. (2006). Modified 2, 2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2, 2′-diphenyl-1-picrylhydrazyl (DPPH) methods. Journal of Agricultural and Food Chemistry, 54(4), 1151–1157.

    Article  CAS  Google Scholar 

  • Peanparkdee, M., Iwamoto, S., Borompichaichartkul, C., Duangmal, K., & Yamauchi, R. (2016). Microencapsulation of bioactive compounds from mulberry (Morus alba L.) leaf extracts by protein–polysaccharide interactions. International Journal of Food Science and Technology, 51(3), 649–655.

    Article  CAS  Google Scholar 

  • Pérez-Vicente, A., Gil-Izquierdo, A., & García-Viguera, C. (2002). In vitro gastrointestinal digestion study of pomegranate juice phenolic compounds, anthocyanins, and vitamin C. Journal of Agricultural and Food Chemistry, 50(8), 2308–2312.

    Article  Google Scholar 

  • Pitija, K., Nakornriab, M., Sriseadka, T., Vanavichit, A., & Wongpornchai, S. (2013). Anthocyanin content and antioxidant capacity in bran extracts of some Thai black rice varieties. International Journal of Food Science and Technology, 48(2), 300–308.

    Article  CAS  Google Scholar 

  • Posuwan, J., Prangthip, P., Leardkamolkarn, V., Yamborisut, U., Surasiang, R., Charoensiri, R., et al. (2013). Long-term supplementation of high pigmented rice bran oil (Oryza sativa L.) on amelioration of oxidative stress and histological changes in streptozotocin-induced diabetic rats fed a high fat diet; Riceberry bran oil. Food Chemistry, 138(1), 501–508.

    Article  CAS  Google Scholar 

  • Prangthip, P., Surasiang, R., Charoensiri, R., Leardkamolkarn, V., Komindr, S., Yamborisut, U., et al. (2013). Amelioration of hyperglycemia, hyperlipidemia, oxidative stress and inflammation in steptozotocin-induced diabetic rats fed a high fat diet by riceberry supplement. Journal of Functional Foods, 5(1), 195–203.

    Article  CAS  Google Scholar 

  • Pratruangkrai, P. (2015). Riceberry plantation to expand. http://www.nationmultimedia.com/business/Riceberry-plantation-to-expand-30272340.html.

  • Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290–4302.

    Article  CAS  Google Scholar 

  • Rawel, H. M., Kroll, J., & Rohn, S. (2001). Reactions of phenolic substances with lysozyme—physicochemical characterisation and proteolytic digestion of the derivatives. Food Chemistry, 72(1), 59–71.

    Article  CAS  Google Scholar 

  • Saikia, S., Mahnot, N. K., & Mahanta, C. L. (2015). Optimisation of phenolic extraction from Averrhoa carambola pomace by response surface methodology and its microencapsulation by spray and freeze drying. Food Chemistry, 171, 144–152.

    Article  CAS  Google Scholar 

  • Saxena, A., Sachin, K., Bohidar, H. B., & Verma, A. K. (2005). Effect of molecular weight heterogeneity on drug encapsulation efficiency of gelatin nano-particles. Colloids and Surfaces B: Biointerfaces, 45(1), 42–48.

    Article  CAS  Google Scholar 

  • Schultz, N., Metreveli, G., Franzreb, M., Frimmel, F. H., & Syldatk, C. (2008). Zeta potential measurement as a diagnostic tool in enzyme immobilisation. Colloids and Surfaces B: Biointerfaces, 66(1), 39–44.

    Article  CAS  Google Scholar 

  • Shahidi, F., & Han, X. Q. (1993). Encapsulation of food ingredients. Critical Reviews in Food Science and Nutrition, 33(6), 501–547.

    Article  CAS  Google Scholar 

  • Shao, Y., Xu, F., Sun, X., Bao, J., & Beta, T. (2014). Identification and quantification of phenolic acids and anthocyanins as antioxidants in bran, embryo and endosperm of white, red and black rice kernels (Oryza sativa L.) Journal of Cereal Science, 59(2), 211–218.

    Article  CAS  Google Scholar 

  • Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40(6), 945–948.

    Article  CAS  Google Scholar 

  • Sitthitool, R.. (2015). Thailand to increase organic Riceberry rice for the world market. http://thaitribune.org/contents/detail/315?content_id=16065&rand=1466156260.

  • Stratil, P., Klejdus, B., & Kuban, V. (2006). Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. Journal of Agricultural and Food Chemistry, 54(3), 607–616.

    Article  CAS  Google Scholar 

  • Sutharut, J., & Sudarat, J. (2012). Total anthocyanin content and antioxidant activity of germinated colored rice. International Food Research Journal, 19(1), 215–221.

    CAS  Google Scholar 

  • Suttiarporn, P., Sookwong, P., & Mahatheeranont, S. (2016). Fractionation and identification of antioxidant compounds from bran of Thai black rce cv. riceberry. International Journal of Chemical Engineering and Applications, 7(2), 109.

    Article  CAS  Google Scholar 

  • Tabaraki, R., & Nateghi, A. (2011). Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology. Ultrasonics Sonochemistry, 18(6), 1279–1286.

    Article  CAS  Google Scholar 

  • Tagliazucchi, D., Verzelloni, E., Bertolini, D., & Conte, A. (2010). In vitro bio-accessibility and antioxidant activity of grape polyphenols. Food Chemistry, 120(2), 599–606.

    Article  CAS  Google Scholar 

  • Tan, Y. T., Peh, K. K., & Al-Hanbali, O. (2000). Effect of Carbopol and polyvinylpyrrolidone on the mechanical, rheological, and release properties of bioadhesive polyethylene glycol gels. AAPS PharmSciTech, 1(3), 69–78.

    Article  Google Scholar 

  • Tananuwong, K., & Tewaruth, W. (2010). Extraction and application of antioxidants from black glutinous rice. LWT-Food Science and Technology, 43(3), 476–481.

    Article  CAS  Google Scholar 

  • Thamnarathip, P., Jangchud, K., Jangchud, A., Nitisinprasert, S., Tadakittisarn, S., & Vardhanabhuti, B. (2016). Extraction and characterisation of Riceberry bran protein hydrolysate using enzymatic hydrolysis. International Journal of Food Science and Technology, 51(1), 194–202.

    Article  CAS  Google Scholar 

  • Waterhouse, A. L. (2001). Determination of total phenolics. In R. E. Wrolstad (Ed.), Current protocols in food analytical chemistry (pp. I1.1.1–I1.1.8). New York: Wiley.

    Google Scholar 

  • Wei, X., Luo, M., Li, W., Yang, L., Liang, X., Xu, L., et al. (2012). Synthesis of silver nanoparticles by solar irradiation of cell-free Bacillus amyloliquefaciens extracts and AgNO 3. Bioresource Technology, 103(1), 273–278.

    Article  CAS  Google Scholar 

  • Wojdyło, A., Oszmiański, J., & Czemerys, R. (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105(3), 940–949.

    Article  Google Scholar 

  • Woodward, G. M., Needs, P. W., & Kay, C. D. (2011). Anthocyanin-derived phenolic acids form glucuronides following simulated gastrointestinal digestion and microsomal glucuronidation. Molecular Nutrition & Food Research, 55(3), 378–386.

    Article  CAS  Google Scholar 

  • Yin, G., Liu, Z., Zhan, J., Ding, F., & Yuan, N. (2002). Impacts of the surface charge property on protein adsorption on hydroxyapatite. Chemical Engineering Journal, 87(2), 181–186.

    Article  CAS  Google Scholar 

  • Zhou, K., & Yu, L. (2004). Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT-Food Science and Technology, 37(7), 717–721.

    Article  CAS  Google Scholar 

  • Zullaikah, S., Lai, C.-C., Vali, S. R., & Ju, Y.-H. (2005). A two-step acid-catalyzed process for the production of biodiesel from rice bran oil. Bioresource Technology, 96(17), 1889–1896.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to express their gratitude to Associate Professor Apichart Vanavichit, Kasetsart University, Thailand, for kindly providing the Riceberry bran, Nitta Gelatin, Inc., for providing the gelatin samples, and JSPS KAKENHI (Grant Number JP26450364) for funding this research.

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  1. Division of Science of Biological Resources, United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Methavee Peanparkdee, Satoshi Iwamoto & Ryo Yamauchi

  2. Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Satoshi Iwamoto & Ryo Yamauchi

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  1. Methavee Peanparkdee
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  2. Satoshi Iwamoto
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Correspondence to Satoshi Iwamoto.

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Peanparkdee, M., Iwamoto, S. & Yamauchi, R. Preparation and Release Behavior of Gelatin-Based Capsules of Antioxidants from Ethanolic Extracts of Thai Riceberry Bran. Food Bioprocess Technol 10, 1737–1748 (2017). https://doi.org/10.1007/s11947-017-1945-9

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