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Apoptosis of RKO induced by catechins and GA through Ca2+ and LIP

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Wuhan University Journal of Natural Sciences

Abstract

The anti-tumor effects of catechins and gallic acid (GA) in-vitro was investigated in this paper. Fluo-3AM, Calcium-AM (Ca-AM), 2′, 7′ -dichlorofluorescein-diacetate(DCFH-DA), 4′, 6-diamidino-2- phenylindole (DAPI) and Ca-AM plus colbat were used to characterize intracellular calcium, labile iron pool (LIP), reactive oxygen species (ROS), nuclei morphology and mitochondrial permeability transition pore (mPTP) opening, respectively. High performance liquid chromatography (HPLC) was used to quantitate catechins and GA in the cultural medium. The results indicated that each of them showed dose response inhibition of cell growth, provoking nuclei condensation, intracellular calcium elevation, mPTP opening, LIP reduction, and cytochrome c (Cyt-C) to release into cytosol. The caspase inhibitors, 2-aminoethoxydiphenol borate (APB) or Fe3+ could inhibit lethal effects of GA and (-)-epigallocatechin (EGC), but failed to affect (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG). Level of ROS presented negative growth while their concentration decreased in the medium. In conclusion, our findings suggest that viability of RKO decreased because of their good correlation with elevation of calcium and loss of LIP and ROS in cytosol.

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Authors and Affiliations

  1. Key Laboratory of Tea Resource Multi-field Applied Technology of Zhejiang Province, 310016, Zhejiang, Hangzhou, China

    Yunfei Tu

  2. Hangzhou Institute of Tea, All China Federtaion of Supply and Marketing Cooperative, 310016, Zhejiang, Hangzhou, China

    Yunfei Tu

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  1. Yunfei Tu
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Correspondence to Yunfei Tu.

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Foundation item: Supported by Zhejiang Provincial Natural Science Foundation of China (Y3100683)

Biography: TU Yunfei, male, M.D., research direction: active components of tea and their anti-cancer activity.

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Tu, Y. Apoptosis of RKO induced by catechins and GA through Ca2+ and LIP. Wuhan Univ. J. Nat. Sci. 19, 341–349 (2014). https://doi.org/10.1007/s11859-014-1023-3

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  • DOI: https://doi.org/10.1007/s11859-014-1023-3

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