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Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance

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Abstract

The Arabidopsis gene AVP1 encodes an H+-pyrophosphatase that functions as a proton pump at the vacuolar membranes, generating a proton gradient across vacuolar membranes, which serves as the driving force for many secondary transporters on vacuolar membranes such as Na+/H+-antiporters. Overexpression of AVP1 could improve drought tolerance and salt tolerance in transgenic plants, suggesting a possible way in improving drought and salt tolerance in crops. The AVP1 was therefore introduced into peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both drought and salt tolerance in greenhouse and growth chamber conditions, as AVP1-overexpressing peanuts produced more biomass and maintained higher photosynthetic rates under both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plants by having higher photosynthetic rates and producing higher yields under low irrigation conditions.

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Abbreviations

AVP1 :

Arabidopsis vacuolar pyrophosphatase 1

IPT:

Isopentenyltransferase

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Acknowledgments

This research was supported by Grants from National Peanut Board and Texas Peanut Producers Board. Hua Qin thanks the China Scholarship Council for a 1-year fellowship to study abroad.

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Authors and Affiliations

  1. College of Life Sciences, Sichuan University, Chengdu, Sichuan, China

    Hua Qin & Yizheng Zhang

  2. Department of Biological Sciences, Texas Tech University, Lubbock, TX, 79409, USA

    Qiang Gu, Sundaram Kuppu, Li Sun, Xunlu Zhu, Neelam Mishra, Rongbin Hu & Hong Zhang

  3. Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China

    Guoxin Shen

  4. Texas AgriLife Research Center, Texas A&M University, Lubbock, TX, 79403, USA

    Junling Zhang & Mark Burow

  5. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China

    Longfu Zhu & Xianlong Zhang

  6. USDA-ARS Cropping Systems Research Laboratory, Lubbock, TX, 79415, USA

    Paxton Payton

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  1. Hua Qin
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  2. Qiang Gu
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  3. Sundaram Kuppu
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  4. Li Sun
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  9. Junling Zhang
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  12. Xianlong Zhang
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  13. Mark Burow
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  14. Paxton Payton
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  15. Hong Zhang
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Correspondence to Hong Zhang.

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Qin, H., Gu, Q., Kuppu, S. et al. Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance. Plant Biotechnol Rep 7, 345–355 (2013). https://doi.org/10.1007/s11816-012-0269-5

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  • DOI: https://doi.org/10.1007/s11816-012-0269-5

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