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Sequential hydrolysis of hemicellulose and lignin in lignocellulosic biomass by two-stage percolation process using dilute sulfuric acid and ammonium hydroxide

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Abstract

To obtain the total fractionation and pretreatment of the corn stover, two-stage percolation process was investigated. This process consists of two steps: use of 0.07 wt% sulfuric acid for hemicellulose recovery in first stage and ARP (ammonia recycled percolation) in the following stage for lignin recovery. Among tested conditions, the best conditions of two-stage process were as follows: 1st stage; 170 °C, 2.5 ml/min, 30 minutes using 0.07 wt% sulfuric acid and 2nd stage; 170 °C, 5.0 ml/min, 60minutes using 15 wt% ammonium hydroxide. At above two-stage treatment conditions, the hemicellulose in corn stover was easily hydrolyzed (95%) and recovered with high yields (86%) and the extent of the lignin removal was 81%. After two-stage process, the treated biomass contained nearly pure glucan (85%). Two-stage treatment brought about enzymatic digestibility of 90% and 89% with 60 and 15 FPU/g glucan cellulase enzyme loadings, respectively.

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Authors and Affiliations

  1. Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, 50011, USA

    Tae Hyun Kim

  2. Department of Natural Resource Ecology and Management, Iowa State University, Ames, IA, 50011, USA

    Tae Hyun Kim

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  1. Tae Hyun Kim
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Correspondence to Tae Hyun Kim.

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Kim, T.H. Sequential hydrolysis of hemicellulose and lignin in lignocellulosic biomass by two-stage percolation process using dilute sulfuric acid and ammonium hydroxide. Korean J. Chem. Eng. 28, 2156–2162 (2011). https://doi.org/10.1007/s11814-011-0093-6

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  • DOI: https://doi.org/10.1007/s11814-011-0093-6

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