Abstract
Two new dihydropyrones, rhytismatones C (1) and D (2), and a known compound, penicillenol A1 (3), were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Penicillium citrinum HDN11-186. Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance (NMR) spectra and mass spectra. The absolute configurations of new compounds were determined by calculating the electronic circular dichroism (ECD) spectrum. UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture, suggesting new biosynthetic pathways were activated in the co-cultured fungi. Compound 1 showed obvious antibacterial activities against Proteus sp. MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration (MIC) both at 25 µmol L−1.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 41806167), the High-Level Talents Research Fund of Qingdao Agricultural University (No. 665/1120034), the NSFC-Shandong Joint Fund (No. U1906212), the Major Project of the 14th Five-Year Plan (No. 2022QNLM030003-1), the Natural Science Foundation of Shandong Province (No. ZR2021ZD28), the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (No. 2021CXLH0012), and the Youth Innovation Plan of Shandong Province (No. 2019KJM004).
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Yu, G., Zhou, L., Wu, G. et al. New Antibacterial Dihydropyrones Induced by Co-Culture of Penicillium crustosum PRB-2 and Penicillium citrinum HDN11-186. J. Ocean Univ. China 23, 216–220 (2024). https://doi.org/10.1007/s11802-023-5676-4
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DOI: https://doi.org/10.1007/s11802-023-5676-4