Abstract
Lipases have been widely applied in a variety of industrial fields, such as food, pharmaceuticals, biofuels, and biotechnology. Recent years have witnessed a great interest in modifying lipids for the production of triacylglycerols enriched with n-3 polyunsaturated fatty acids (PUFAs). Here, a novel salt-tolerant, organic solvent-stable, and bile salt-activated lipase was purified from golden pompano (Trachinotus ovatus) viscera, which was named as golden pompano lipase (GPL). GPL had a specific activity of 57.2 Umg−1 with an estimated molecular weight of 14 kDa, exhibited optimal activity at 40°C and pH 8.0, and showed Km and Vmax of 40.16 µmolL−1 and 769.23 µmolL−1 min−1, respectively. GPL activity was enhanced by Mn2+ and sodium deoxycholate. It was active in organic solvents, including methanol, ethanol, chloroform, and hexane. GPL also showed a good salinity tolerance of up to 1 molL−1. n-3 PUFA enrichment in the glyceride fraction of golden pompano oil was performed by GPL-catalyzed hydrolysis and yielded a total PUFA concentration of 56.99%. EPA, DHA, and DPA were enriched by 10.4-, 3.2-, and 1.8-fold of their initial levels, respectively. This study recognized the industrial applicability of GPL to prepare enriched C20−22n-3 PUFA.
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Acknowledgements
This work was supported by the National Key R&D Programs of China (No. 2018YFD0901103), the Program of the Hainan Association for Science and Technology Plans to Youth R&D Innovation (No. QCXM202003), and the Hainan Provincial Natural Science Foundation of China (No. 2019RC093).
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Liu, H., Liu, S., Zhang, X. et al. Novel Lipase from Golden Pompano (Trachinotus ovatus) Viscera: Purification, Characterization, and Application in the Concentrating of n-3 Polyunsaturated Fatty Acids. J. Ocean Univ. China 22, 501–508 (2023). https://doi.org/10.1007/s11802-023-5234-0
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DOI: https://doi.org/10.1007/s11802-023-5234-0