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Sialoglycoproteins Isolated from the Eggs of Gadus morhua Inhibit Bone Resorption in Ovariectomized Rats by Suppressing the MAPK and NF-κB Pathways

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Abstract

The present study investigated the anti-osteoporosis function and the mechanism of sialoglycoproteins isolated from the eggs of Gadus morhua (Gm-SGP) on ovariectomized (OVX) rats. After 3 months of Gm-SGP treatment, OVX-induced bone loss was suppressed and uncoupling bone turnover was balanced, as indicated by systemic biomarkers of bone metabolism; no uterine estrogenicity was observed. Moreover, rats administered with Gm-SGP exhibited increased bone mineral density and biomechanical strength and significant restoration of the trabecular microarchitecture compared with rats in the control group. Gm-SGP significantly decreased bone resorption-related indicators in serum. Investigation of the associated mechanisms revealed that Gm-SGP significantly increases the OPG/RANKL ratio at the mRNA and protein levels. Further research suggested that Gm-SGP inhibits the mRNA and protein expressions of important transcription factors of the MAPK and NF-κB signaling pathways. It also attenuates the activation of related transduction signaling pathways by inhibiting phosphorylation of JNK, ERK, p38, and NF-κB, and ultimately suppresses the induction of c-Fos and NFATc1. Overall, these results demonstrate that Gm-SGP inhibits bone resorption by suppressing osteoclastogenesis-related MAPK and NF-κB pathways, thereby improving osteoporosis.

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Acknowledgments

This study is financially supported by the National Natural Science Foundation of China (No. 31371876).

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  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Lei Mao, Yiming Wang, Meiling Wang, Yufeng Dai, Jingfeng Wang & Changhu Xue

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  1. Lei Mao
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  2. Yiming Wang
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  3. Meiling Wang
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Correspondence to Jingfeng Wang or Changhu Xue.

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Mao, L., Wang, Y., Wang, M. et al. Sialoglycoproteins Isolated from the Eggs of Gadus morhua Inhibit Bone Resorption in Ovariectomized Rats by Suppressing the MAPK and NF-κB Pathways. J. Ocean Univ. China 18, 1174–1184 (2019). https://doi.org/10.1007/s11802-019-3881-y

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