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Preparation of magnetic gelatin-starch microspheres and adsorption performance for bovine serum album

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Abstract

The magnetic gelatin-starch microspheres were prepared by modified emulsion cross-linking method with glutaraldehyde as the cross-linking agent. The structure, size distribution as well as morphology of magnetic microspheres were investigated by FT-IR spectrometer, dynamic laser scattering analyzer and scanning electron microscope, respectively. Bovine serum album (BSA) was chosen as model protein, and the adsorption processes were carried out under diversified conditions including BSA initial concentration, pH value, adsorption time and temperature to evaluate the performance of the magnetic microspheres. The average diameter of optimized spherical magnetic microspheres is 1.6 μm with excellent dispersivity, and the saturation magnetization is found to be equal to 1.056×10−2 A·m2. The adsorption isotherm of the BSA on the magnetic microspheres basically obeys the Langmuir model, with a maximum adsorption capacity of 120 mg/g and an adsorption equilibrium constant of 1.60 mL/mg.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan, 528458, China

    Qiu-xing He  (何秋星)

  2. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Ping Song  (宋平)

  3. School of Chinese Materi Medica, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Zhi-ping Zhang  (张智萍) & Zhuo-ying You  (尤卓莹)

  4. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, China

    Wei-ping Tu  (涂伟萍)

Authors
  1. Qiu-xing He  (何秋星)
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  2. Ping Song  (宋平)
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  3. Zhi-ping Zhang  (张智萍)
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  4. Zhuo-ying You  (尤卓莹)
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Corresponding author

Correspondence to Qiu-xing He  (何秋星).

Additional information

Foundation item: Project(GC201204) supported by the Open Fund of Guangdong Provincial Key Laboratory for the Green Chemicals, China

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He, Qx., Song, P., Zhang, Zp. et al. Preparation of magnetic gelatin-starch microspheres and adsorption performance for bovine serum album. J. Cent. South Univ. 22, 1220–1226 (2015). https://doi.org/10.1007/s11771-015-2636-1

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  • DOI: https://doi.org/10.1007/s11771-015-2636-1

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