Abstract
Auricularia is a genus of edible jelly fungi whose highly plastic fruiting bodies lack differentiation between stalks and caps, making accurate identification of similar species difficult. Auricularia villosula (A. villosula) is a wild edible mushroom that is also used in traditional Chinese medicine. Here, we sequenced and assembled its mitochondrial genome using Illumina short reads. The complete sequence is 230,069 bp in length with a GC content of 30.1%. It harbors 41 genes (25 tRNA, 2 rRNA, and 14 protein-coding): 2 on the N strand ( −) and 29 on the J strand ( +). Analysis of nucleotide composition revealed positive GC (0.027) and AT (0.002) skews. The mitochondrial genome included a very large intergenic region (22,840 bp) between cytochrome b (CytB) and tRNATyr, lacked overlapping nucleotides between genes, and used non-standard start codons for some genes. Phylogenetic analysis revealed that A. villosula was distant from other species of Polyporales, Agaricales, and Russulales. The A. villosula mitogenome sequence will be useful for future taxonomic and genetic studies.
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Data Availability
The mitochondrial genome data have been deposited at NCBI with accession number OM998506. The data are available from the corresponding authors upon reasonable request.
Abbreviations
- A. villosula :
-
Auriculariavillosula
- CytB:
-
Cytochrome b
- ATP:
-
Adenosine triphosphate
- PCGs:
-
Protein-coding genes
- tRNAs:
-
Transfer ribonucleic acids
- ORFs:
-
Open reading frames
- DNA:
-
Deoxyribonucleic acid
- NCBI:
-
National Center for Biotechnology Information
- ITS:
-
Internal transcribed spacer
- NGS:
-
Next generation sequencing
- QC:
-
Quality control
- RSCU:
-
Relative synonymous codon usage
- Ks:
-
Synonymous
- Ka:
-
Nonsynonymous
- NADH:
-
Nicotinamide adenine dinucleotide
- DHU:
-
Dihydrouridine
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Funding
This research was supported by the China Agriculture Research system [CRAS20], the Modern Agriculture and Innovation of Agricultural Organization System of Guangxi [nycytxgxcxtd-20–02], the Science and Technology Fund of Guangxi Academy of Agricultural Sciences [2021JM88, 2023YM07, and 2021YT094] and the Guangxi Natural Science Foundation [2019JJA130125].
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Conceptualization, supervision, and funding acquisition, Xiaoguo Wang and Liangliang Qi; resources, Shiyan Wei and Shengjin Wu; investigation, Xiaoguo Wang and Jiaojun Wei; writing—review and editing Xiaoguo Wang, Jun Tang and Zengliang Liu. All authors have read and agreed to the published version of the manuscript.
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Wang, X., Wei, S., Wu, S. et al. Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Auricularia villosula, an edible wild mushroom in China. Biologia 78, 3713–3723 (2023). https://doi.org/10.1007/s11756-023-01533-0
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DOI: https://doi.org/10.1007/s11756-023-01533-0