Skip to main content

Advertisement

SpringerLink
Log in

Tissue culture of Indian rosewood (Dalbergia latifolia Roxb.)

  • Review
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

Dalbergia latifolia Roxb. (Fabaceae), famed for its wood, is a vulnerable plant species according to the International Union for Conservation of Nature and Natural Resources (IUCN) Red List as a result of its over-utilisation. This mini-review, which provides a synthesis of the micropropagation of this woody plant species, can be useful for the establishment of a more focused and comprehensive micropropagation protocol. An in-depth analysis of previously published reports indicates that the most commonly employed explant in D. latifolia in vitro regeneration protocols are seedling-derived explants such as cotyledonary nodes since the use of ex vitro material leads to considerable contamination and phenolic oxidation. The media composition for culture initiation, multiplication, maintenance and rooting has been thoroughly studied but in most studies, pertinent information about acclimatization and plant survival in the field was not provided, reducing the reproducibility of protocols. This mini-review provides possible solutions to overcome explant contamination and browning during D. latifolia in vitro culture establishment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Batista DS, Felipe SHS, Silva TD, de Castro KM, Mamedes-Rodrigues TC, Miranda NA, Ríos-Ríos AM, Faria DV, Fortini EA, Chagas K, Torres-Silva G, Xavier A, Arencibia AD, Otoni WC (2018) Light quality in plant tissue culture: does it matter? In Vitro Cell Dev Biol Plant 54(3):195–215. https://doi.org/10.1007/s11627-018-9902-5

    Article  CAS  Google Scholar 

  • Beyl CA (2011) Getting started with tissue culture: media preparation, sterile technique, and laboratory equipment. In: Trigiano RN, Gray DJ (eds) Plant tissue culture, development, and biotechnology. CRC Press Inc, Boca Raton, pp 11–26

    Google Scholar 

  • Boga A, Ram B, Ravi G, Reddy S (2012) Effect of benzyl amino purine and gibberellic acid on in vitro shoot multiplication and elongation of Dalbergia latifolia Roxb.: an important multipurpose tree. Biotechnol Bioinforma Bioeng 2(1):597–602

  • Cardoso JC, Gerald LTC, Teixeira da Silva JA (2018) Micropropagation in the twenty-first century. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Plant cell culture protocols, methods in molecular biology, vol 1815. Springer Nature, The Netherlands, pp 17–46. https://doi.org/10.1007/978-1-4939-8594-4_2

    Chapter  Google Scholar 

  • Chaturvedi HC, Sharma M, Sharma AK, Jain M, Agha BQ, Gupta P (2004) In vitro germplasm preservation through regenerative excised root culture for conservation of phytodiversity. Indian J Biotechnol 3(2):305–315

    Google Scholar 

  • Hazarika BN, Teixeira da Silva JA, Talukdar A (2006) Effective acclimatization of in vitro cultured plants: methods, physiology and genetics. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol II, 1st edn. Global Science Books Ltd, Isleworth, pp 427–438

  • IUCN (2020) The IUCN Red List of Threatened Species (2020) Dalbergia latifolia Roxb. In: Asian Reg. Work. (Conservation Sustain. Manag. Trees, Viet Nam, August 1996). https://doi.org/10.2305/IUCN.UK.1998.RLTS.T32098A9675296.en. Accessed 31 Aug 2021

  • Jøker D (2004) Dalbergia latifolia Roxb. In: Seed Leaflet, pp 1–2

  • Kacar YA, Byrne PF, Teixeira da Silva JA (2006) Molecular markers in plant tissue culture. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol II, 1st edn. Global Science Books Ltd, Isleworth, pp 444–449

    Google Scholar 

  • Kher MM, Nataraj M, Teixeira da Silva JA (2016) Micropropagation of Crataeva L. species. Rend Lincei 27(2):157–167. https://doi.org/10.1007/s12210-015-0478-2

    Article  Google Scholar 

  • Kovalchuk IY, Mukhitdinova Z, Turdiyev T, Madiyeva G, Akin M, Eyduran E, Reed BM (2018) Nitrogen ions and nitrogen ion proportions impact the growth of apricot (Prunus armeniaca) shoot cultures. Plant Cell Tissue Organ Cult 133(2):263–273. https://doi.org/10.1007/s11240-018-1379-8

    Article  CAS  Google Scholar 

  • Lakshmi Sita G, Chattopadhyay S, Tejavathi DH (1986) Plant regeneration from shoot callus of rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep 5(4):266–268. https://doi.org/10.1007/BF00269818

    Article  CAS  PubMed  Google Scholar 

  • Lakshmi Sita G, Raghava Swamy BV (1992) Application of cell and tissue culture technology for mass propagation of elite trees with special reference to rosewood (Dalbergia latifolia Roxb.). Indian For 118(1):36–47

  • Lakshmi Sita G, Raghava Swamy BV (1993) Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci 88(1):107–112. https://doi.org/10.1016/0168-9452(93)90115-G

    Article  Google Scholar 

  • Leifert C, Woodward S (1998) Laboratory contamination management: the requirement for microbiological quality assurance. Plant Cell Tissue Organ Cult 52(1):83–88. https://doi.org/10.1023/A:1005905604043

    Article  Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Int Plant Propagators’ Soc Proc 30:421–427

    Google Scholar 

  • Muralidhar Rao M, Lakshmi Sita G (1996) Direct somatic embroygenesis from immature embryos of rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep 15(5):355–359. https://doi.org/10.1007/BF00232371

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Nataraja K, Sudhadevi AM (1984) Induction of plantlets from seedling explants of Dalbergia latifolia Roxb. in vitro. Beitrage Zur Biol Der Pflanz 59:341–349

    Google Scholar 

  • Negi KS (1982) A note on rooting response in Dalbergia latifolia Roxb. cuttings by plant hormones. Indian For 108(4):300–301

    CAS  Google Scholar 

  • Niedz RP, Evens TJ (2016) Design of experiments (DOE) – history, concepts, and relevance to in vitro culture. In Vitro Cell Dev Biol Plant 52(6):547–562. https://doi.org/10.1007/s11627-016-9786-1

    Article  CAS  Google Scholar 

  • Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S (2009) Dalbergia latifolia. In: Agroforestree Database a tree Ref. Sel. Guid. version 4.0. http://www.worldagroforestry.org/treedb/AFTPDFS/Dalbergia_latifolia.PDF. Accessed 31 Aug 2021

  • Pijut PM, Beasley RR, Lawson SS, Palla KJ, Stevens ME, Wang Y (2012) In vitro propagation of tropical hardwood tree species - A review (2001-2011). Propag Ornam Plants 12(1):25–51

  • Pospíšilová J, Tichá I, Kadlechek P, Haisel D, Plzáková Š (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42(4):481–497. https://doi.org/10.1023/A:1002688208758

    Article  Google Scholar 

  • Preece JE (1995) Can nutrient salts partially substitute for plant growth regulators? Plant Tissue Cult Biotechnol 1(1):26–37

    Google Scholar 

  • Preece JE (2003) A century of progress with vegetative plant propagation. HortScience 38(5):1015–1025. https://doi.org/10.21273/hortsci.38.5.1015

    Article  Google Scholar 

  • Preece JE (2010) Micropropagation in stationary liquid media. Propag Ornam Plants 10(4):183–187

    Google Scholar 

  • Preece JE, Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Springer, Dordrecht, pp 71–93. https://doi.org/10.1007/978-94-009-2075-0_5

    Chapter  Google Scholar 

  • Pradhan C, Pattnaik S, Chand PK (1998a) Rapid in vitro propagation of East Indian rosewood (Dalbergia latifolia Roxb.) through high frequency shoot proliferation from cotyledonary nodes. J Plant Biochem Biotechnol 7(1):61–64. https://doi.org/10.1007/BF03263037

    Article  CAS  Google Scholar 

  • Pradhan C, Pattnaik S, Dwari M, Patnaik SN, Chand PK (1998b) Efficient plant regeneration from cell suspension-derived callus of East Indian rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep 18(1):138–142. https://doi.org/10.1007/s002990050546

  • Raghava Swamy BV, Himabindu K, Sita GL (1992) In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep 11(3):126–131. https://doi.org/10.1007/BF00232164

  • Ravishankar Rai V (1993) Embryogenesis through cell suspension culture of forest tree: Dalbergia latifolia Roxb. Plant Tissue Cult 3:23–27

    Google Scholar 

  • Ravishankar Rai V, Jagadish Chandra KS (1988) In vitro regeneration of plantlets from shoot callus of mature trees of Dalbergia latifolia. Plant Cell Tissue Organ Cult 13(1):77–83. https://doi.org/10.1007/BF00043048

    Article  Google Scholar 

  • Ravishankar Rai V, Jagadish Chandra KSS (1989) Micropropagation of Indian Rosewood by tissue culture. Ann Bot 64(1):43–46. https://doi.org/10.1093/oxfordjournals.aob.a087806

    Article  Google Scholar 

  • Sankara Rao K (1986) Plantlets from somatic callus tissue of the East Indian rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep 5(3):199–201. https://doi.org/10.1007/BF00269118

  • Sankara Rao K, Lakshmi Sita G, Vaidyanathan CS (1985) In vitro cloning of Dalbergia latifolia Roxb. (Indian rosewood). In: Henke RR, Hughes KW, Constantin MJ, Hollaender A, Wilson CM (Eds) Tissue Culture in Forestry and Agriculture, Volume 32 of the series Basic Life Sciences. Springer US, Boston, MA, p 346 (Abstract). https://doi.org/10.1007/978-1-4899-0378-5_52

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50(1):199–204. https://doi.org/10.1139/b72-026

    Article  CAS  Google Scholar 

  • Sharma PK (2006) Production of synthetic seeds in Dalbergia latifolia Roxb. J Phytol Res 19(2):333–334

    Google Scholar 

  • Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology – a complete synthesis. Biotechnol Adv 31(2):186–207. https://doi.org/10.1016/j.biotechadv.2012.09.007

    Article  CAS  PubMed  Google Scholar 

  • Shekhawat NS, Rathore TS, Singh RP, Deora NS, Rao SR (1993) Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regul 12:273–280. https://doi.org/10.1007/BF00027208

    Article  CAS  Google Scholar 

  • Shirin F, Bhadrawale D, Mishra JP, Sonkar MK, Maravi S (2020) Evaluation of biochemical changes during different stages of somatic embryogenesis in a vulnerable timber tree Dalbergia latifolia (Indian rosewood). In Vitro Cell Dev Biol Plant 56(6):894–902. https://doi.org/10.1007/s11627-020-10099-4

    Article  CAS  Google Scholar 

  • Skirvin RM, McPheeters KD, Norton M (1994) Sources and frequency of somaclonal variation. HortScience 29(11):1232–1237. https://doi.org/10.21273/HORTSCI.29.11.1232

    Article  Google Scholar 

  • Subasinghe S, Warakagoda PS, Abeywickrama WHT (2015) In vitro shoot proliferation of rosewood (Dalbergia latifolia: Fabaceae): a potent high quality timber species. Trop Agric Res Ext 18(2):94–101. https://doi.org/10.4038/tare.v18i2.5329

    Article  Google Scholar 

  • Sudhadevi AM, Nataraja K (1987a) In vitro regeneration and establishment of plantlets in stem cultures of Dalbergia latifolia Roxb. Indian for 113(7):501–506

    Google Scholar 

  • Sudhadevi AM, Nataraja K (1987b) Establishment of plantlets in hypocotyl cultures of Dalbergia latifolia Roxb. Indian J for 10:1–6

    Google Scholar 

  • Teixeira da Silva JA (2012a) Is BA (6-benzyladenine) BAP (6-benzylaminopurine)? Asian Australasian J Plant Sci Biotechnol 6 (special issue 1):121–124

  • Teixeira da Silva JA (2012b) Callus, calluses or calli: multiple plurals? Asian Australasian J Plant Sci Biotechnol 6 (special issue 1):125–126

  • Teixeira da Silva JA, Kulus D, Zhang X, Zeng S-J, Ma G-H, Piqueras A (2016a) Disinfection of explants for saffron (Crocus sativus L.) tissue culture. Env Exp Biol 14(4):183–198. https://doi.org/10.22364/eeb.14.25

    Article  Google Scholar 

  • Teixeira da Silva JA, Kher MM, Soner D, Page T, Zhang X, Nataraj M, Ma GH (2016b) Sandalwood: basic biology, tissue culture, and genetic transformation. Planta 243(4):847–887. https://doi.org/10.1007/s00425-015-2452-8

    Article  CAS  PubMed  Google Scholar 

  • Teixeira da Silva JA, Kher MM, Soner D, Nataraj M (2018) Indian kino tree (Pterocarpus marsupium): propagation, micropropagation, and biotechnology. Environ Exp Biol 16(1):1– 8. https://doi.org/10.22364/eeb.16.01

  • Teixeira da Silva JA, Kher MM, Soner D, Nataraj M (2019) Red sandalwood (Pterocarpus santalinus L. f.): biology, importance, propagation and micropropagation. J For Res 30(3):745–754. https://doi.org/10.1007/s11676-018-0714-6

    Article  Google Scholar 

  • Teixeira da Silva JA, Nezami-Alanagh E, Barreal ME, Kher MM, Wicaksono A, Gulyás A, Hidvégi N, Magyar-Tábori K, Mendler-Drienyovszki N, Márton L, Landín M, Gallego PP, Driver JA, Dobránszki J (2020) Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions. Planta 252(3):47. https://doi.org/10.1007/s00425-020-03449-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tewari DN (1995) Monograph on Rosewood (Dalbergia latifolia Roxb.). International Book Distributors, Dehra Dun, pp 1–74

  • Thimijan RW, Heins RD (1983) Photometric, radiometric, and quantum light units of measure: a review of procedures for interconversion. HortScience 18(6):818–822

    Google Scholar 

  • Vasava D, Kher MM, Nataraj M, Teixeira da Silva JA (2018) Bael tree (Aegle marmelos (L.) Corrêa): importance, biology, propagation, and future perspectives. Trees 32(5):1165–1198. https://doi.org/10.1007/s00468-018-1754-4

  • Verma P, Soni KK, Verma RK, Shirin F (2016) Seedling dieback of Dalbergia latifolia in Central India caused by Colletotrichum capsici—a new record. Int J Curr Microbiol App Sci 5(6):350–356. https://doi.org/10.20546/ijcmas.2016.506.040

  • Warrier KCS, Vijaykumar NK (1999) Clonal propagation of selected plus trees of Indian Rosewood (Dalbergia latifolia Roxb.) through tissue culture. In: Srivastava GC, Singh K, Pal M (eds) Plant physiology for sustainable agriculture. Pointer Publishers, Jaipur, pp 422–432

    Google Scholar 

  • Warrier KCS, Warrier RR, Vijaykumar NK (2005) Polyphenol interference in micropropagation of Indian rosewood (Dalbergia latifolia Roxb.). MyForest 41(4):561–566

  • White PR (1963) The culture of animal and plant cells. The Ronaid Press Co., New York, pp 59

Download references

Author information

Authors and Affiliations

  1. School of Science, GSFC University, Vadodara, Gujarat, 391750, India

    Mafatlal M. Kher

  2. P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, P. O. Box no. 39, Vallabh Vidyanagar, Gujarat, 388120, India

    M. Nataraj

  3. Silviculture and Forest Management Division, Institute of Wood Science and Technology, Malleswaram, Bengaluru, 560 003, Karnataka, India

    A. N. Arun Kumar

  4. Department of Biology, Morgan State University, 1700 E. Cold Spring Lane, Baltimore, MD, 21251, USA

    Viji Sitther

  5. Department of Botany, Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, 605 008, India

    Mahipal S. Shekhawat

  6. Plant Biotechnology & Cytogenetics Division, Institute of Forest Genetics and Tree Breeding, Forest Campus, R.S. Puram, P.B. No. 1061, Coimbatore, 641 002, India

    Rekha R. Warrier

  7. Independent researcher, P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, 761-0799, Kagawa-Ken, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Mafatlal M. Kher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Nataraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. N. Arun Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Viji Sitther
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mahipal S. Shekhawat
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rekha R. Warrier
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jaime A. Teixeira da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed equally to the development of this review.

Corresponding author

Correspondence to Mafatlal M. Kher.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest to declare.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kher, M.M., Nataraj, M., Arun Kumar, A.N. et al. Tissue culture of Indian rosewood (Dalbergia latifolia Roxb.). Biologia 76, 3595–3604 (2021). https://doi.org/10.1007/s11756-021-00914-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11756-021-00914-7

Keywords

Search

Navigation