Abstract
Acca sellowiana (Myrtaceae) is a multipurpose species with edible fruits and ornamental value, native to Uruguay and southern Brazil. Domestication of the species in Uruguay is incipient although in other countries, it is widely cultivated. It is an evergreen out-crossing shrub, pollinated by birds and bees. For this reason, it is necessary to develop vegetative propagation strategies such as stem cuttings to reproduce outstanding genotypes for conservation or breeding programs. Adventitious root (AR) formation in cuttings is regulated by environmental and endogenous factors. Among phytohormones, indole-butyric acid (IBA) is the most widely exogenous auxin used to improve rooting of cuttings. Most studies on AR formation at the molecular level use model species; however, the conservation of these mechanisms in non-model plants has been little studied, consequently the effects of different factors and their interactions in A. sellowiana are not well understood. The identification and expression analysis of genes known to be involved in the regulation of the process is an important step to elucidate the molecular mechanisms that regulate AR differentiation in A. sellowiana cuttings. In this study, we compared two genotypes with contrasting rooting ability, and we identified and characterized three genes that might regulate the onset of AR development in A. sellowiana: AsPIN1, AsTIR1 and AsSHR. Their expression analysis showed that in the difficult-to-root genotype, AsTIR1 increases strongly in response to exogenous IBA, shortly after induction treatment. Relative expression of AsPIN1 and AsSHR also increases 24 h later. The biological significance of this gene expression pattern is discussed.
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Data availability
The datasets generated and analyzed during the current study are available in the GenBank repository at https://www.ncbi.nlm.nih.gov/genbank/, reference number MZ130946-MZ13095.
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Acknowledgements
This research was funded by AGENCIA NACIONAL DE INVESTIGACIÓN E INNOVACIÓN; grant number FMV-2-2011-1-6320. Mother plants from the breeding program of Acca sellowiana used in this study were kindly provided by Ing. Agr. (M.Sc.) Danilo Cabrera (Instituto Nacional de Investigación Agropecuaria, Canelones, Uruguay) and Ing. Agr. Beatriz Vignale (Facultad de Agronomía, Salto, Uruguay).
Funding
The research leading to these results received funding from AGENCIA NACIONAL DE INVESTIGACIÓN E INNOVACIÓN under Grand Agreement FMV-2–2011-1–6320.
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Conceptualization: SR and PS; Methodology: SR, SR-D; JPS and GP; Formal analysis and investigation: SR; Writing—original draft preparation: SR; Writing—review and editing: SR, PS and OB; Funding acquisition: SR and PS; Project administration: SR; Supervision: PS.
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Ross, S., Rodríguez-Decuadro, S., Pérez, G. et al. Validation and expression analysis of candidate genes for adventitious rooting, in micro-cuttings of Acca sellowiana (Myrtaceae). Acta Physiol Plant 46, 53 (2024). https://doi.org/10.1007/s11738-024-03682-4
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DOI: https://doi.org/10.1007/s11738-024-03682-4