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Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

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Abstract

Plant tissue culture can readily be applied to the field of medicinal plants and secondary metabolites production. In this study, in vitro propagation protocol of Rumex cyprius Murb. was established. The highest shoot multiplication rate was observed from explants grown on MS medium supplemented with 1.5 mg/L BA (7.8 shoots per explant). For rooting, the maximum root induction rate was observed on MS medium supplemented with 1.5 mg/L IBA or 1.0 mg/L NAA. The longest roots were observed for plants grown on MS medium supplemented with 1.5 mg/L IBA. For callus induction, leaves from in vitro grown plants were cultured in various levels of 2,4-D or different combinations of BA and NAA. The highest fresh weight (5.86 g) of calli was observed at 1.5 and 0.5 mg/L BA and NAA, respectively. The second part of this work investigated the effects of abiotic factors (NaCl and Mannitol) and biotic factors (Yeast extract and Chitosan) on secondary metabolites accumulation of in vitro grown R. cyprius. Phenolic compounds were extracted, and the individual phenolic compounds and antioxidant activity of the extracts were determined of in vitro grown R. cyprius. The total phenol content and antioxidant activity increased significantly by the pervious factors. Reversed high-pressure liquid chromatography (RP-HPLC) was used to measure the effect of these factors on the contents of individual phenolic compound. New phenolic compounds (Gallic acid and Chlorogenic acid) not found in R. cyprius plants grown on control media were produced in R. cyprius plants grown on media supplemented with elicitors. Results indicate that using elicitors in plant tissue culture could be used to produce/enhance secondary metabolites accumulation in R. cyprius.

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Abbreviations

BA:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

TDZ:

Thidiazuron

Kin:

Kinetin

NAA:

1-Naphthaleneacetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog (1962) medium

References

  • Abbade L, Paiva P, Paiva R et al (2010) Growth curve and biochemical analysis of callus of IPE-BRANCO (Tabebuia roseo alba). Naturalia 33:45–56

    Google Scholar 

  • Abo El-soud IH, Al-azab A, Koriesh EM et al (2011) Micropropagation of Rumex vesicarius L. through shoot tip culture. Catrina J 6:41–45

    Google Scholar 

  • Al Khateeb W, Hussein E, Qouta L et al (2012) In vitro propagation and characterization of phenolic content along with antioxidant and antimicrobial activities of Cichorium pumilum Jacq. Plant Cell Tissue Organ Cult 110:103–110

    Article  CAS  Google Scholar 

  • Al Zghoul H (2012) Production of phytochemical compounds from in vitro grown Rumex cyprius L. and Artemisia herba alba L. M.Sc Thesis in Department of Biological Sciences, Yarmouk University, Jordan

  • Alu’datt M, Alli I, Ereifej K et al (2010) Optimisation, characterisation and quantification of phenolic compounds in olive cake. Food Chem 123:117–122

    Article  Google Scholar 

  • Aytekin A, Morimura S, Kida K (2011) Synthesis of chitosan caffeic acid derivatives and evaluation of their antioxidant activities. J Biosci Bioeng 111:212–216

    Article  CAS  PubMed  Google Scholar 

  • Balaraju K, Agastian P, Ignacimuthu S (2009) Micropropagation of swertia chirata Buch.-Hams. ex Wall.: a critically endangered medicinal herb. Acta Physiol Plant 31:487–494

    Article  Google Scholar 

  • Bourgaud F, Gravot A, Milesi S et al (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851

    Article  CAS  Google Scholar 

  • Canter PH, Thomas H, Ernst E (2005) Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotech 23:180–185

    Article  CAS  Google Scholar 

  • Chanda S, Dave R (2009) In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. Afr J Microbiol Res 3:981–996

    Google Scholar 

  • Chen H, Chena F, Chiu F et al (2001) The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza. Enzym Microb Technol 28:100–105

    Article  CAS  Google Scholar 

  • Chengalrayan K, Abouzid A, Gallo-Meacher M (2005) In vitro regeneration of plants from sugarcane seed-derived callus. Vitro Cell Dev Biol Plant 41:477–482

    Article  Google Scholar 

  • Cunha A, Ferreira M (1996) Somatic embryogenesis, organogenesis and callus growth kinetics of flax (Linum usitatissimum L.). Plant Cell Tissue Organ Cult 47:1–8

    Article  Google Scholar 

  • Dawidowicz A, Wianowska D, Baraniak B (2006) The antioxidant properties of alcoholic extracts from Sambucus nigra L. (antioxidant properties of extracts). LWT Food Sci Technol 39:308–315

    Article  CAS  Google Scholar 

  • Dicosmo F, Misawa M (1985) Eliciting secondary metabolism in plant cell cultures. Trends Biotech 3:318–322

    Article  CAS  Google Scholar 

  • Dornenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzym Microb Technol 17:674–684

    Article  Google Scholar 

  • Erkan N, Ayranci G, Ayranci E (2008) Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 110:76–82

    Article  CAS  PubMed  Google Scholar 

  • Estime L, O'Shea M, Borst M et al (2002) Suspension culture and plant regeneration of Typha latifolia. HortScience 37:406–408

    Google Scholar 

  • Gouthamchandra K, Mahmood R, Manjunatha H (2010) Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ Toxicol Pharmacol 30:11–18

    Article  CAS  PubMed  Google Scholar 

  • Hintikka V (1990) Germination ecology and survival strategy of Rumex acetosella polygonaceae on drought exposed rock outcrops in south finland. Ann Bot Fenn 27:205–215

    Google Scholar 

  • Jaleel C, Gopi R, Lakshmanan G (2006) Triadimefon induced changes in the antioxidant metabolism and ajmalicine production in Catharanthus roseus (L.) G. Don. Plant Sci 171:271–276

    Article  CAS  Google Scholar 

  • Moncalean P, Rodriguez A, Fernandez B (2003) Effect of different benzyladenine time pulses on the endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in micropropagated explants of Actinidia deliciosa. Plant Physiol Bioch 41:149–155

    Article  CAS  Google Scholar 

  • Mostafa H, Elbakry A, Alam E (2011) Evaluation of antibacterial and antioxidant activities of different plant parts of Rumex vesicariusl. Int J Pharm Pharm Sci 3:109–118

    Google Scholar 

  • Nhut D, Trang T, Vu N et al (2005) A wounding method and liquid culture in Paphiopedilum delenatii propagation. Propag Ornam Plants 5:156–161

    Google Scholar 

  • Norton M, Norton C (1985) In vitro propagation of Ericaceae: a comparison of the activity of the cytokinins N6-benzyladenine and N6-isopentenyladenine in shoot proliferation. Sci Hortic 27:335–340

    Article  CAS  Google Scholar 

  • Pradeep K, Park S, Ko K (2008) Hesperidin a flavanoglycone protects against -irradiation induced hepatocellular damage and oxidative stress in Sprague Dawley rats. Eur J Pharmacol 587:273–280

    Article  CAS  PubMed  Google Scholar 

  • Purkayastha J, Sugla T, Paul A et al (2008) Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant. Vitro Cell Dev Biol Plant 44:442–447

    Article  CAS  Google Scholar 

  • Saleh N, El-Hadidi M, Arafa R (1993) Flavonoids and anthraquinones of some Egyptian Rumexspecies (Polygonaceae). Biochem Syst Ecol 21:301–303

    Article  CAS  Google Scholar 

  • Simon S, Petrasek J (2011) Why plants need more than one type of auxin. Plant Sci 180:454–460

    Article  CAS  PubMed  Google Scholar 

  • Singh J, Tiwari K (2012) In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L. An important medicinal plant. Ind Crops Prod 35:224–229

    Article  CAS  Google Scholar 

  • Singh S, Lewis N, Towers G (1998) Nitrogen recycling during phenylpropanoid metabolism in sweet potato tubers. J Plant Physiol 153:316–323

    Article  CAS  PubMed  Google Scholar 

  • Sujatha G, Kumari B (2008) Micropropagation, encapsulation and growth of Artemisia vulgaris node explants for germplasm preservation. S Afr J Bot 74:93–100

    Article  CAS  Google Scholar 

  • Thokozani B, Zulu D, Sileshi G (2011) Seed germination and in vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis. Afr. J. Biotechnol 10:5959–5966

    CAS  Google Scholar 

  • Thomas T, Maseena E (2006) Callus induction and plant regeneration in Cardiospermum halicacabum Linn. an important medicinal plant. Sci Hortic 108(3):332–336

    Article  CAS  Google Scholar 

  • Tripathi L, Tripathi J (2003) Role of biotechnology in medicinal plants. Trop J Pharm Res 2:243–253

    Google Scholar 

  • Van Assche J, Van Nerum D, Darius P (2002) The comparative germination ecology of nine Rumex species. Plant Ecol 159:131–142

    Article  Google Scholar 

  • Vieira C, Silva E, Alvarenga A et al (2010) Stress-associated factors increase after desiccation of germinated seeds of Tabebuia impetiginosa Mart. Plant Growth Regul 62:257–263

    Article  CAS  Google Scholar 

  • Vinale F, Sivasithamparam K, Ghisalberti E (2008) A novel role for Trichoderma secondary metabolites in the interactions with plants. Physiol Mol Plant Pathol 72:80–86

    Article  CAS  Google Scholar 

  • Wahid A, Ghazanfar A (2006) Possible involvement of some secondary metabolites in salt tolerance of sugarcane. J Plant Physiol 163:723–730

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Hu Z, Wang Y (2011) Phenolic compounds and the antioxidant activities in litchi pericarp: difference among cultivars. Sci Hortic 129:784–789

    Article  CAS  Google Scholar 

  • Warghat A, Rampur N, Wagh P (2011) In vitro Callus induction of Abelmoschus moschatus L. by using different hormone concentration. Int J Pharm Sci Rev Res 10:82–84

    CAS  Google Scholar 

  • Xing X, Ono A, Miyanaga K (2001) A kinetic model for growth of callus derived from Eucommia ulmoides aiming at mass production of a factor enhancing collagen synthesis of animal cells. Math Comput Simul 56:463–474

    Article  Google Scholar 

  • Yang J, Guo J, Yuan J (2008) In vitro antioxidant properties of rutin. Food Sci Technol 41:1060–1066

    CAS  Google Scholar 

  • Zeng S, Wu K, Silva T (2012) Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Sci Hortic 138:198–209

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Adel Rababah for his technical assistance. This study was supported by the deanship of research at Yarmouk University/Jordan as M.Sc. thesis project for Haifa Al Zghoul. Funding was provided by Yarmouk University (Grant No. 12/2011).

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Authors and Affiliations

  1. Department of Biological Sciences, Yarmouk University, Irbid, Jordan

    Wesam Al Khateeb, Haifa Al Zghoul, Razan Kanaan, Ahmad El-Oqlah & Jamil Lahham

  2. Department of Food Science, Jordan University of Science and Technology, Irbid, Jordan

    Muhammad Alu’datt

Authors
  1. Wesam Al Khateeb
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  2. Muhammad Alu’datt
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  3. Haifa Al Zghoul
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  4. Razan Kanaan
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  5. Ahmad El-Oqlah
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  6. Jamil Lahham
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Correspondence to Wesam Al Khateeb.

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Communicated by Q. Wang.

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Al Khateeb, W., Alu’datt, M., Al Zghoul, H. et al. Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.. Acta Physiol Plant 39, 14 (2017). https://doi.org/10.1007/s11738-016-2312-6

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  • DOI: https://doi.org/10.1007/s11738-016-2312-6

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