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From green to red: large-scale transcriptome comparison of a bud sport in poplar (Populus deltoides)

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An Erratum to this article was published on 31 March 2017

Abstract

The red-leaf coloration of Quanhong poplar (QHP) (Populus deltoides), resulting from a bud sport, is a distinctive trait conferring high ornamental value. However, the underlying molecular coloration mechanism of the red-leaf poplar remains unknown. In this study, tissue buds and leaves from mutant QHP(red)and its wild-type progenitor L2025 (green) were used to study the coloration mechanism by high-throughput RNA sequencing and comparative analysis of the transcriptomes. A total of 12,000 differentially expressed genes (DEGs) were obtained. Functional enrichment using Gene Ontology (GO) and, Genes and Genomes (KEGG) annotations showed that 4951 bud DEGs and 1927 leaf DEGs participate in many important biological and metabolic pathways, including anthocyanin biosynthesis. Importantly, some structural genes that contribute to photosynthesis were down-regulated, and some candidate genes involved in the biosynthesis of abscisic acid (ABA), ethylene and anthocyanin were up-regulated in the QHP mutant. The DEGs identified provided insight into the novel traits of the mutant. The unigene dataset that was used to discover candidate genes provides a comprehensive resource for molecular research in red-leaf poplar and may provide insight into coloration of other woody plants.

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Acknowledgments

The authors thank Professor Cheng XJ and Zhou CS for providing the materials used in this study. This work was supported by the National Natural Science Fund of China (No. 31300514) and by the 12th Five Year Key Programs for forest breeding in Sichuan Province (No. 2011YZGG).

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Authors and Affiliations

  1. College of Landscape Architecture, Sichuan Agricultural University, 611130, Chengdu, China

    Fan Zhang, Jinglong Zhao, Wei Li, Lingxia Sun & Qibing Chen

  2. College of Forestry, Sichuan Agricultural University, 611130, Chengdu, China

    Xueqin Wan & Xiaomei Luo

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  1. Fan Zhang
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  2. Jinglong Zhao
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Correspondence to Xueqin Wan.

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Communicated by J.-H. Liu.

An erratum to this article is available at http://dx.doi.org/10.1007/s11738-017-2406-9.

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Zhang, F., Zhao, J., Wan, X. et al. From green to red: large-scale transcriptome comparison of a bud sport in poplar (Populus deltoides). Acta Physiol Plant 38, 244 (2016). https://doi.org/10.1007/s11738-016-2259-7

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