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Quantitative analysis of phosphorus, iron, and arsenic in the inner and outer portions of rice roots: an interaction of arsenic with iron

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Abstract

Two experiments were conducted to observe the effect of arsenic (As; NaAsO2) and additional Fe3+ (Fe3+-EDTA) on phosphorus (P), iron (Fe), and As in inner and outer portions of rice roots (Oryza sativa L. cv. Akitakomachi) grown for 14 days. Measurement of elements was done with PIXE (particle-induced X-ray emission) system. The first experiment contained various rates of As ranging from 0 to as high as 26.8 µM with a constant rate of 10 µM Fe3+, while in the second experiment, the Fe concentrations were varied from 10 to 50 µM with As at 0 and 13.4 µM. The intensity of the reddish coloration in the roots increased with increasing As in the medium. Phosphorus adsorbed by apoplastic Fe and Fe-plaque (outer portion) was not detected, suggesting that P might not have complexed with apoplastic Fe or with Fe-plaque. Iron was mostly concentrated in the outer portion of the roots whereas As was mostly concentrated in the inner portion. High-Fe3+ in the medium caused a decrease in P and As concentrations in the inner portion of the roots.

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Abbreviations

CBE:

Citrate-bicarbonate-ethylenediaminetetraacetic acid

DCB:

Dithionite-citrate-bicarbonate

DAT:

Days after treatments

DW:

Dry weight

PIXE:

Particle-induced X-ray emission

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Acknowledgments

Dr. M. R. Shaibur wishes to express his appreciation to the Ministry of Education, Science, Culture and Sports, Government of Japan for providing him a Monbukagakusho Scholarship during which period this study was undertaken. We are grateful to Mr. TA Adjadeh of University of Ghana for correcting the English of the manuscript.

Conflict of interest

The authors declared that they have no conflict of interest.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan

    Molla Rahman Shaibur & Shigenao Kawai

  2. Department of Soil, Water and Environment, University of Dhaka, Dhaka, 1000, Bangladesh

    S. M. Imamul Huq

Authors
  1. Molla Rahman Shaibur
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  2. S. M. Imamul Huq
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  3. Shigenao Kawai
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Correspondence to Molla Rahman Shaibur.

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Communicated by W. Filek.

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Shaibur, M.R., Imamul Huq, S.M. & Kawai, S. Quantitative analysis of phosphorus, iron, and arsenic in the inner and outer portions of rice roots: an interaction of arsenic with iron. Acta Physiol Plant 37, 67 (2015). https://doi.org/10.1007/s11738-015-1815-x

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  • DOI: https://doi.org/10.1007/s11738-015-1815-x

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