Abstract
Conventional breeding methods are now supplemented by modern in vitro techniques. However, long term maintenance of cultures has many disadvantages. It incurs risk of loss through microbial contamination, somaclonal variation or human error, but above all the regeneration capacity can decrease gradually during extended maintenance. Cryopreservation as a method of long term storage of biological material without genetic alteration was adapted for embryogenic triticale calli preservation. Callus of both winter and spring genotypes were successfully cryopreserved. The best viability rates (80–85%) were achieved with 6 weeks old winter genotypes treated with cryoprotective solution containing DMSO. This simple and efficient method of cryopreservation requires no special devices for controlled freezing and can be easily adapted for other cereals.
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References
Bajaj Y.P.S. 1984. The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor. Appl. Genet., 67: 525–528.
Chen T.H.H., Kartha K.K, Gusta L.V. 1985. Cryopreservation of wheat suspension culture and regenerable callus. Plant Cell Tiss. Org. Cult., 4: 101–109
Cornejo M.J., Wong V.L., Blechl A.E. 1995. Cryopreserved callus: a source of protoplasts for rice transformation. Plant Cell Rep., 14: 210–214.
Datta K., Potrykus I., Datta S.K. 1992. Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.). Plant Cell Rep., 11: 229–233.
De Buyser J., Marcotte J-L., Henry Y. 1992. Genetic analysis of in vitro wheat somatic embryogenesis. Euphytica, 63: 265–270.
DeVerno L.L., Park Y.S., Bonga J.M., Barrett J.D. 1999. Somaclonal variation in cryopreserved embryogenic clones of white spruce [Picea glauca (Moench) Voss.]. Plant Cell Rep., 18:948–953
Fretz A., Lörz H. 1995. Cryopreservation of in vitro cultures of barley (Hordeum vulgareL. and H. murinum L.) and transgenic cells of wheat (Triticum aestivum). J. Plant Physiol., 146:489–496
Fahay G.M., Levy D.J., Ali S.E. 1987. Some engineering principles undarlaying the physical properties, biological actions and utility of vitrification solutions. Cryobiology, 24: 196–213.
Gnanapragasam S., Vasil I.K. 1992. Cryopreservation of immature embryos, embryogenic callus and cell suspension culture of gramineous species. Plant Sci., 83: 205–215.
Gordon-Kamm W.J., Spencer T.M., Mangano M.L., Adams T.R., Daines R.J., Start W.G., O’Brian J.V., Chambers S.A., Adams W.R. Jr., Willets N.G., Rice T.B., Mackey C.J., Kruger R.W., Kausch A.P., Lemaux P.G. 1990. Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell, 2: 603–618.
Hahne G., Lörz H. 1987. Cryopreservation of embryogenic callus-cultures from barley (Hordeum vulgare L.). Plant Breed., 99: 330–332.
Jähne A., Lazzeri P.A., Jäger-Gussen M., Lörz H. 1991. Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor. Appl. Genet., 82: 74–80.
Kartha K.K. 1985. Cryopreservation of Plant Cells and Organs. CRC Press. Boca Raton.
Kendall E.J., Kartha K.K, Qureshi J.A, Chermak P. 1993. Cryopreservation of immature spring wheat zygotic embryos using an abscisic acid pretreatment. Plant Cell Rep., 12: 89–94.
Levlock J.E., Bishop M.W.H. 1959. Prevention of freezing damage to living cells by dimethylsulfoxide. Nature, 183: 1394–1395.
Lu T.G., Sun C.S. 1992. Cryopreservation of millet (Setaria indica L.). J. Plant Physiol., 139: 295–298.
Martinez-Montero M.E., Mora N. Quinones J. Gonzalez-Arnao M.T., Engelmann F. Lorenzo J.C. 2002. Effect of cryopreservation on the structural and functional integrity of cell membranes of sugarcane (Saccharum sp.) embryogenic calluses. Cryo-Letters, 23: 237–244
Mazur P. 1984. Freezing of living cells: mechanism and application. Am. J. Physiol., 247: 125–142.
McFarlane D.R. 1987. Physical aspects of vitrification in aqueos solutions. Cryobiology, 24:181–195
Meijer E.G.M., van Iren F., Hansgens L.A.M., Schilperoort R.A. 1991. Retention of the capacity to produce plants from protoplasts in cryopreserved cell lines of rice (Oryza sativa L.). Plant Cell Rep., 10:171–174.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tabaco tissue cultures. Physiol. Plant., 15: 473–497.
Sharma G.C., Bello L.L., Sapra V.T.,1980. Genotypic differences in organogenesis from callus of ten Triticale lines. Euphytica, 29:751–754.
Shillito R.D., Carswell G.K., Johnson C.M., di Aaio J.J., Harms C.T. 1989. Regeneration of fertile plants from protoplasts of elite inbred maize. Biol/Technol., 7:581–587.
Steponkus P.L., Lynch D.V. 1989. The behaviour of large unilamellar vesicles of rye plasma membrane lipids during freeze thaw-induced osmotic excursions. Cryo-Lett., 10:43–50
Turner S., Krauss S.L., Bunn E., Senaratna T., Dixon K., Tan B., Touchell D. 2001. Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation. Plant Science 161:1099–1106.
Zhuang J.J, Jia X. 1983. Cell and Tissue Culture Techniques for Cereal Crop Improvement. Science Press, Beijing 431.
Zimny J., Lörz H. 1989. High frequency of somatic embryogenesis nad plant regeneration of rye (Secale cereale). Plant Breeding, 102:89–100
Zimny J., 1989. Genotypic dependance of the somatic embryogenesis of triticale (x Triticosecale Wittmack). In: Science for Plant Breeding, XII Eucarpia Congress, Göttingen, Germany. 7–13
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Sowa, S., Oleszczuk, S. & Zimny, J. A simple and efficient method for cryopreservation of embryogenic triticale calli. Acta Physiol Plant 27, 237–243 (2005). https://doi.org/10.1007/s11738-005-0028-0
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DOI: https://doi.org/10.1007/s11738-005-0028-0