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Sorghum lysine-rich cultivar verification by SDS-PAGE and Southern blot

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Abstract

The aim of this study was to assess the genetic variability among lysine-rich cultivars of sorghum (IS 21702; CVS B65, G 1058, G205 and CVS 549) and to compare with low lysine cultivar White Martin and a chemically induced high lysine mutant P721O. The lysine-rich cultivars contain approximately 1.5 to 2 times more lysine when compared to low-lysine cultivar. Sodium-dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of kafirins showed the absence of both 25.3 kD and 25.9 kD α-kafirins in lysine rich cultivars IS 21702, G 1058 and CV 365 and only the 25.9 kD protein was not present in G 205 compared with a low-lysine cultivar White Martin and with a chemically induced high-lysine mutant P721O. Southern blot analysis with RsaI enzyme gave significantly different banding pattern indicating the absence of 1.0 kb band in lysine-rich cultivars IS 21702, G 1058 and CVS 365 compared to White Martin indicating genetic variability among these cultivars. The detected variability among kafirins both in SDS-PAGE and Southern blot could be effectively used as markers in selection of lysine-rich cultivars for further use in breeding programme.

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Author notes

  1. N. P. Eswara Reddy (Associate Professor)

    Present address: S.V. Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati, Andhra Pradesh, India

Authors and Affiliations

  1. Laboratorium voor Plantengenetica, Instituut voor Moleculaire Biologie, Vrije Universiteit Brussels, B-1640, Sint-Genesius Rode, Belgium

    N. P. Eswara Reddy (Associate Professor) & M. Jacobs

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  1. N. P. Eswara Reddy
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  2. M. Jacobs
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Eswara Reddy, N.P., Jacobs, M. Sorghum lysine-rich cultivar verification by SDS-PAGE and Southern blot. Acta Physiol Plant 24, 285–290 (2002). https://doi.org/10.1007/s11738-002-0053-1

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  • DOI: https://doi.org/10.1007/s11738-002-0053-1

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