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BcDR1, a putative gene, regulates the development and pathogenicity of Botrytis cinerea

  • Research Article
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Frontiers of Agriculture in China

Abstract

Botrytis cinerea is one of the important phytopathogenic fungi. Cloning of the genes related to their development and pathogenicity is fundamental to the pathogen control. A mutant (BCt160), which produces abnormal conidia and no sclerotia, was identified from Botrytis cinerea mutant library generated by Agrobacterium tumefaciens mediated transformation (ATMT). Southern blotting analysis showed that one T-DNA insertion occurred in the genome of the mutant. TAIL-PCR (thermal asymmetric interlaced PCR) and bioinformatic analysis indicated that the exogenous T-DNA insertion occurred in the second exon of a putative gene BC1G_12388.1, named as BcDR1 (B. cinerea development-related gene 1). The function analysis of BcDR1 gene showed that the BcDR1 was related to development, morphological differentiation, and pathogenicity of B. cinerea, suggesting that BcDR1 gene was required for the development and pathogenicity of B. cinerea.

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References

  • Bahn Y S, Xue C, Idnurm A, Rutherford J C, Heitman J, Cardenas M E (2007). Sensing the environment: lessons from fungi. Nat Rev Microbiol, 5(1): 57–69

    Article  PubMed  CAS  Google Scholar 

  • Balhadère P V, Foster A J, Talbot N J (1999). Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea by insertional mutagenesis. Mol Plant Microbe Interact, 12(2): 129–142

    Article  Google Scholar 

  • Brito N, Espino J J, Gonzalez C (2006). The endo-beta-1,4-xylanase xyn11A is required for virulence in Botrytis cinerea. Mol Plant Microbe Interact, 19(1): 25–32

    Article  PubMed  CAS  Google Scholar 

  • Choquer M, Fournier E, Kunz C, Levis C, Pradier J M, Simon A, Viaud M (2007). Botrytis cinerea virulence factors: new insights into a necrotrophic and polyphageous pathogen. FEMS Microbiol Lett, 277(1): 1–10

    Article  PubMed  CAS  Google Scholar 

  • Cui Z, Ding Z, Yang X, Wang K, Zhu T (2009). Gene disruption and characterization of a class V chitin synthase in Botrytis cinerea. Can J Microbiol, 55(11): 1267–1274

    Article  PubMed  CAS  Google Scholar 

  • de Groot M J, Bundock P, Hooykaas P J, Beijersbergen A G (1998). Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol, 16(9): 839–842

    Article  PubMed  Google Scholar 

  • Drenth A, Goodwin S B, Fry W E, Davidse L C (1993). Genotypic diversity of Phytophthora infestans in the Netherlands revealed by DNA polymorphisms. Phytopathology, 83(10): 1087–1092

    Article  CAS  Google Scholar 

  • Elad Y, Williamson B, Tudzynski P, Delen N (2004) Botrytis spp. And Diseases They Cause in Agricultural Systems—An Introduction. In: Elad Y, Williamson B, Tudzynski P, Delen N, eds. Botrytis: Biology, Pathology and Control. the Netherlands: Kluwer Academic Publishers

    Google Scholar 

  • Fillinger S, Chaveroche M K, Shimizu K, Keller N, D’Enfert C (2002). cAMP and ras signalling independently control spore germination in the filamentous fungus Aspergillus nidulans. Mol Microbiol, 44(4): 1001–1016

    Article  PubMed  CAS  Google Scholar 

  • Jurick W N II, Rollins J A (2007). Deletion of the adenylate cyclase (sac1) gene affects multiple developmental pathways and pathogenicity in Sclerotinia sclerotiorum. Fungal Genet Biol, 44(6): 521–530

    Article  PubMed  CAS  Google Scholar 

  • Kars I, McCalman M, Wagemakers L, van Kan J A (2005). Functional analysis of Botrytis cinerea pectin methylesterase genes by PCR-based targeted mutagenesis: Bcpme1 and Bcpme2 are dispensable for virulence of strain B05.10. Mol Plant Pathol, 6(6): 641–652

    Article  PubMed  CAS  Google Scholar 

  • Lazo G R, Stein P A, Ludwig R A (1991). A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Nat Biotechnol, 9(10): 963–967

    Article  CAS  Google Scholar 

  • Liebmann B, Gattung S, Jahn B, Brakhage A A (2003). cAMP signaling in Aspergillus fumigatus is involved in the regulation of the virulence gene pksP and in defense against killing by macrophages. Mol Genet Genomics, 269(3): 420–435

    Article  PubMed  CAS  Google Scholar 

  • Michielse C B, Hooykaas P J J, Hondel C A M J J, Ram A F J (2005). Agrobacterium-mediated transformation as a tool for functional genomics in fungi. Curr Genet, 48(1): 1–17

    Article  PubMed  CAS  Google Scholar 

  • Mullins E D, Chen X, Romaine P, Raina R, Geiser D M, Kang S (2001). Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology, 91(2): 173–180

    Article  PubMed  CAS  Google Scholar 

  • Nierman WC, Pain A, Anderson M J, Wortman J R, Kim H S, Arroyo J, Berriman M, Abe K, Archer D B, Bermejo C, Bennett J, Bowyer P, Chen D, Collins M, Coulsen R, Davies R, Dyer P S, Farman M, Fedorova N, Fedorova N, Feldblyum T V, Fischer R, Fosker N, Fraser A, García J L, García M J, Goble A, Goldman G H, Gomi K, Griffith-Jones S, Gwilliam R, Haas B, Haas H, Harris D, Horiuchi H, Huang J, Humphray S, Jiménez J, Keller N, Khouri H, Kitamoto K, Kobayashi T, Konzack S, Kulkarni R, Kumagai T, Lafton A, Latgé J P, Li W, Lord A, Lu C, Majoros W H, May G S, Miller B L, Mohamoud Y, Molina M, Monod M, Mouyna I, Mulligan S, Murphy L, O’Neil S, Paulsen I, Peñalva M A, Pertea M, Price C, Pritchard B L, Quail M A, Rabbinowitsch E, Rawlins N, Rajandream M A, Reichard U, Renauld H, Robson G D, de Córdoba S R, Rodríguez-Peña J M, Ronning C M, Rutter S, Salzberg S L, Sanchez M, Sánchez-Ferrero J C, Saunders D, Seeger K, Squares R, Squares S, Takeuchi M, Tekaia F, Turner G, de Aldana C R V, Weidman J, White O, Woodward J, Yu J H, Fraser C, Galagan J E, Asai K, Machida M, Hall N, Barrell B, Denning D W (2005). Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature, 438(7071): 1151–1156

    Article  PubMed  CAS  Google Scholar 

  • Piers K L, Heath J D, Liang X, Stephens K M, Nester E W (1996). Agrobacterium tumefaciens-mediated transformation of yeast. Proc Natl Acad Sci USA, 93(4): 1613–1618

    Article  PubMed  CAS  Google Scholar 

  • Rivera M C, Lopez M V, Lopez S E (2009). Mycobiota from Cyclamen persicum and its interaction with Botrytis cinerea. Mycologia, 101(2): 173–181

    Article  PubMed  CAS  Google Scholar 

  • Rolland S, Jobic C, Fevre M, Bruel C (2003). Agrobacterium-mediated transformation of Botrytis cinerea, simple purification of monokaryotic transformants and rapid conidia-based identification of the transfer-DNA host genomic DNA flanking sequences. Curr Genet, 44(3): 164–171

    Article  PubMed  CAS  Google Scholar 

  • Rui O, Hahn M (2007). The Slt2-type MAP kinase Bmp3 of Botrytis cinerea is required for normal saprotrophic growth, conidiation, plant surface sensing and host tissue colonization. Mol Plant Pathol, 8(2): 173–184

    Article  PubMed  CAS  Google Scholar 

  • Segmuller N, Ellendorf U, Tudzynski B, Tudzynski P (2007). BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea. Eukaryot Cell, 6(2): 211–221

    Article  PubMed  Google Scholar 

  • Takano Y, Komeda K, Kojima K, Okuno T (2001). Proper regulation of cyclic AMP-dependent protein kinase is required for growth, conidiation, and appressorium function in the anthracnose fungus Colletotrichum lagenarium. Mol Plant Microbe Interact, 14(10): 1149–1157

    Article  PubMed  CAS  Google Scholar 

  • Tellier F, Fritz R, Kerhoas L, Ducrot P H, Einhorn J, Carlin-Sinclair A, Leroux P (2008). Characterization of metabolites of fungicidal cymoxanil in a sensitive strain of Botrytis cinerea. J Agric Food Chem, 56(17): 8050–8057

    Article  PubMed  CAS  Google Scholar 

  • Thevelein J M, Gelade R, Holsbeeks I, Lagatie O, Popova Y, Rolland F, Stolz F, van de Velde S, van Dijck P, Vandormael P, van Nuland A, van Roey K, van Zeebroeck G, Yan B (2005). Nutrient sensing systems for rapid activation of the protein kinase A pathway in yeast. Biochem Soc Trans, 33(1): 253–256

    Article  PubMed  CAS  Google Scholar 

  • Tudzynski P, Siewers V (2004). Approaches to Molecular Genetics and Genomics of Botrytis. In: Elad Y, Williamson B, Tudzynski P, Delen N, eds. Botrytis: Biology, Pathology and Control. The Netherlands: Kluwer Academic Press, 53–66

    Google Scholar 

  • van der Vlugt-Bergmans C J, Wagemakers C A, van Kan J A (1997). Cloning and expression of the cutinase A gene of Botrytis cinerea. Mol Plant Microbe Interact, 10(1): 21–29

    Article  PubMed  Google Scholar 

  • van Kan J A (2006). Licensed to kill: the lifestyle of a necrotrophic plant pathogen. Trends Plant Sci, 11(5): 247–253

    Article  PubMed  Google Scholar 

  • Vienken K, Fischer R (2006). The Zn(II)2Cys6 putative transcription factor NosA controls fruiting body formation in Aspergillus nidulans. Mol Microbiol, 61(2): 544–554

    Article  PubMed  CAS  Google Scholar 

  • Williamson B, Tudzynski B, Tudzynski P, van Kan J A (2007). Botrytis cinerea: the cause of grey mould disease. Mol Plant Pathol, 8(5): 561–580

    Article  PubMed  CAS  Google Scholar 

  • Yamauchi J, Takayanagi N, Komeda K, Takano Y, Okuno T (2004). cAMP-pKA signaling regulates multiple steps of fungal infection cooperatively with Cmk1 MAP kinase in Colletotrichum lagenarium. Mol Plant Microbe Interact, 17(12): 1355–1365

    Article  PubMed  CAS  Google Scholar 

  • Zhao W, Panepinto J C, Fortwendel J R, Fox L, Oliver B G, Askew D S, Rhodes J C (2006). Deletion of the regulatory subunit of protein kinase A in Aspergillus fumigatus alters morphology, sensitivity to oxidative damage, and virulence. Infect Immun, 74(8): 4865–4874

    Article  PubMed  CAS  Google Scholar 

  • Zheng L, Campbell M, Murphy J, Lam S, Xu J R (2000). The BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea. Mol Plant Microbe Interact, 13(7): 724–732

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding, 071001, China

    Bin Zhao, Zhiying Sun, Jihong Xing & Jingao Dong

  2. Agriculture Bureau of Langfang, Langfang, 065000, China

    Meng Zheng

  3. Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050031, China

    Zhiyong Li

Authors
  1. Bin Zhao
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  2. Meng Zheng
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  3. Zhiying Sun
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  4. Zhiyong Li
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  5. Jihong Xing
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  6. Jingao Dong
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Correspondence to Jihong Xing or Jingao Dong.

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They contributed equally to this work.

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Zhao, B., Zheng, M., Sun, Z. et al. BcDR1, a putative gene, regulates the development and pathogenicity of Botrytis cinerea . Front. Agric. China 5, 338–343 (2011). https://doi.org/10.1007/s11703-011-1090-6

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  • DOI: https://doi.org/10.1007/s11703-011-1090-6

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