Abstract
Residual sludge produced at sewage plants is high in water content and contains organic matter in large quantities. In addition, residual sludge is rich in pathogens and bacteria, and the processing cost is usually high. The humus-activated sludge process can improve sludge characteristics and reduce the processing cost for residual sludge in the subsequent stages. We prepared a novel type of composite fillers using the humus-activated sludge process. We comprehensively studied the influence of composite fillers on sludge settling and dewatering performance. The mechanism underlying composite fillers influence on extracellular polymeric substance (EPS) was revealed. Hence, the mechanism underlying composite fillers influence activated sludge settling, and dewatering performance was elucidated from the perspective of EPS. The results showed that as the amount of composite fillers added increased, the sludge volume index (SVI) and capillary suction time (CST) decreased. When the amount of composite fillers added was 100 g, the SVI and CST decreased to 37.5 mL/g and 43.9 s, respectively. Compared with the control group, the settling and dewatering performance increased by 35.3% and 31.8%, respectively. The composite fillers had an impact on the loosely bound extracellular polymeric substances (LB-EPSs), resulting in a significant reduction in polysaccharide (PS) and protein (PN) content and a significant increase in the PN-to-PS ratio. The content of hydroxyl and C–O radicals decreased dramatically. The sludge hydrophilicity decreased as well. The percentages of hydroxide, carbonyl, amido, and alkoxy radicals in protein increased significantly, increasing cation-binding sites. The present study provides a theoretical basis and technical support for improving sludge characteristics using the humus-activated sludge process and reducing the cost of residual sludge processing.
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References
Basuvaraj M, Fein J, Liss SN (2015) Protein and polysaccharide content of tightly and loosely bound extracellular polymeric substances and the development of a granular activated sludge floc. Water Res 82:104–117. https://doi.org/10.1016/j.watres.2015.05.014
Bura R, Cheung M, Liao B, Finlayson J, Lee BC, Droppo IG, Leppard GG, Liss SN (1998) Composition of extracellular polymeric substances in the activated sludge floc matrix. Water Sci Technol 37:325–333
Choi YG, Chung TH (2000) Effects of humus soil on the settling and dewatering characteristics of activated sludge. Water Sci Technol 42:127–134
Feng LJ, Wang JJ, Liu SC, Sun XD, Wang SG (2018) Role of extracellular polymeric substances in the acute inhibition of activated sludge by polystyrene nanoparticles. Environ Pollut 238:859–865. https://doi.org/10.1016/j.envpol.2018.03.101
Gaudy AF (1962) Colorimetric determination of protein and carbohydrate. Ind Water Waste 7:17–22
Higgins MJ, Novak JT (1997) The effect of cations on the settling and dewatering of activated sludges: laboratory results. Water Environ Res 69:215–224. https://doi.org/10.2175/106143097X125371
Jang YS, Kim HS, Ham SY, Park JH, Park HD (2021) Investigation of critical sludge characteristics for membrane fouling in a submerged membrane bioreactor Role of soluble microbial products and extracted extracellular polymeric substances. Chemosphere 271:129879. https://doi.org/10.1016/j.chemosphere.2021.129879
Jin B, Wilén BM, Lant P (2004) Impacts of morphological, physical and chemical properties of sludge flocs on dewaterability of activated sludge. Che Eng J 98:115–126. https://doi.org/10.1016/j.cej.2003.05.002
Jin X, Zhang WJ, Hou R, Jin PK, Song J, Wang XCC (2019) Tracking the reactivity of ozonation towards effluent organic matters from WWTP using two-dimensional correlation spectra. J Environ Sci 76:292–301. https://doi.org/10.1016/j.jes.2018.05.012
Lee MK, Yun YM, Kim DH (2019) Enhanced economic feasibility of excess sludge treatment acid fermentation with biogas production. BMC Energy 1:1–10. https://doi.org/10.1186/s42500-019-0001-x
Li XY, Yang SF (2007) Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge. Water Res 41:1022–1030. https://doi.org/10.1016/j.watres.2006.06.037
Li J, Liu X, Liu Y, Ramsay J, Yao CC, Dai RH (2011) The effect of continuous exposure of copper on the properties and extracellular polymeric substances (EPS) of bulking activated sludge. Environ Sci Pollut Res 18:1567–1573. https://doi.org/10.1007/s11356-011-0492-6
Li CH, Zhao FC, Yu QS, Chen YQ, Zhang CH, Ge Y (2019) Effcts of extracellular polymeric substances on the accumulation and transformation of arsenic by Chlamydomonas reinhardtii. J Hunan Agr U (natural Sciences) 45:384–390. https://doi.org/10.13331/j.cnki.jhau.2019.04.009 (in Chinese)
Li ZW, Lin L, Liu X, Wan CL, Lee DJ (2020) Understanding the role of extracellular polymeric substances in rheological properties of aerobic granular sludge. Sci Total Environ 705:135948. https://doi.org/10.1016/j.scitotenv.2019.135948
Liu XW, Sheng GP, Yu HQ (2009) Physicochemical characteristics of microbial granules. Biotechnol Adv 27:1061–1070. https://doi.org/10.1016/j.biotechadv.2009.05.020
Mohite BV, Koli SH, Patil SV (2018) Heavy metal stress and its consequences on exopolysaccharide (EPS)-producing Pantoea agglomerans. Appl Biochem Biotechnol 186:199–216. https://doi.org/10.1007/s12010-018-2727-1
Murthy SN, Novak JT, Haas RDD (1998) Monitoring cations to predict and improve activated sludge settling and dewatering properties of industrial wastewaters. Water Sci Technol 38:119–126
Peng SN, Hu AB, Ai J, Zhang WJ, Wang DS (2021) Changes in molecular structure of extracellular polymeric substances (EPS) with temperature in relation to sludge macro-physical properties. Water Res 201:117316. https://doi.org/10.1016/j.watres.2021.117316
Pevere A, Guibaud G, Van Hullebusch ED, Boughzala W, Lens PNL (2007) Effect of Na+ and Ca2+ on the aggregation properties of sieved anaerobic granular sludge. Colloid Surface A 306:142–149. https://doi.org/10.1016/j.colsurfa.2007.04.033
Raunkjær K, Hvitved-Jacobsen T, Nielsen PH (1994) Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res 28:251–262. https://doi.org/10.1016/0043-1354(94)90261-5
Sajjad M, Kim KS (2015) Studies on the interactions of Ca2+ and Mg2+ with EPS and their role in determining the physicochemical characteristics of granular sludges in SBR system. Process Biochem 50:966–972. https://doi.org/10.1016/j.procbio.2015.02.020
Sang L (2017) Study on treatment efficienc and microbiological mechanisms of A-OSA process strengthened by composite humic biological filler. Harbin Institute of Technology, Harbin (in Chinese)
Sheng GP, Yu HQ, Li XY (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894. https://doi.org/10.1016/j.biotechadv.2010.08.001
Sponza DT (2003) Investigation of extracellular polymer substances (EPS) and physicochemical properties of different activated sludge flocs under steady-state conditions. Enzyme Microb Tech 32:375–385
Tan PN, Hilal N, Hankins NP, Novak JT (2008) The relationship between cation ions and Polysaccharide on the floc formation of synthetic and activated sludge. Desalination 227:94–102. https://doi.org/10.1016/j.desal.2007.05.038
Urbain V, Block JC, Manem J (1993) Bioflocculation in activated sludge an analytic approach. Water Res 27:829–838
Uyguner CS, Bekbolet M (2005) Evaluation of humic acid photocatalytic degradation by UV-vis and fluorescence spectroscopy. Catal Today 101:267–274. https://doi.org/10.1016/j.cattod.2005.03.011
Verlicchi P, Aukidy MA, Jelic A, Petrović M, Barceló D (2014) Comparison of measured and predicted concentrations of selected pharmaceuticals in wastewater and surface water A case study of a catchment area in the Po Valley (Italy). Sci Total Environ 470–471:844–854. https://doi.org/10.1016/j.scitotenv.2013.10.026
Wang ZC, Gao MC, Zhe W, She ZL, Chang QB, Sun CQ, Zhang J, Ren Y, Yang N (2013) Effect of salinity on extracellular polymeric substances of activated sludge from an anoxic–aerobic sequencing batch reactor. Chemosphere 93:2789–2795. https://doi.org/10.1016/j.chemosphere.2013.09.038
Wu L (2014) Study on the performance and kinetics of nitrogen and phosphorus removal in humic activated sludge A2/O system. Harbin Institute of Technology, Harbin (in Chinese)
Wu M, Zhu R, Pan XH, Sun JP, Zhu Y (2009) Physicochemical characteristics of humus soil. Ind Water Wastewater 40:61–63 (in Chinese)
Wu M, Zhu R, Zhu HG, Dai XH, Yang J (2013) Phosphorus removal and simultaneous sludge reduction in humus soil sequencing batch reactor treating domestic wastewater. Chem Eng J 125–126:136–143. https://doi.org/10.1016/j.cej.2012.11.066
Wu JW, Yang Q, Luo W, Sun J, Xu QX, Chen F, Zhao JW, Yi KX, Wang XL, Wang DB, Li XM, Zeng GM (2018) Role of free nitrous acid in the pretreatment of waste activated sludge extracellular polymeric substances disruption or cells lysis. Chem Eng J 336:28–37. https://doi.org/10.1016/j.cej.2017.11.038
Xiong DS, Xiong DW (2018) Status and research progress on sludge reduction technologies. IOP Conf Ser 170:032066. https://doi.org/10.1088/1755-1315/170/3/032066
Yuan DQ, Wang YL, Feng J (2014) Contribution of stratified extracellular polymeric substances to the gel-like and fractal structures of activated sludge. Water Res 56:56–65. https://doi.org/10.1016/j.watres.2014.02.028
Zhang JK (2015) Study on nitrogen and phosphorus removal and functional bacteria in complex humic biological filler SBR system. Harbin Institute of Technology, Harbin (in Chinese)
Zhang WJ, Cao BD, Wang DS, Ma T, Xia H, Yu DH (2016) Influence of wastewater sludge treatment using combined peroxyacetic acid oxidation and inorganic coagulants re-flocculation on characteristics of extracellular polymeric substances (EPS). Water Res 88:728–739. https://doi.org/10.1016/j.watres.2015.10.049
Zhang YS, Cao BD, Ren RJ, Shi Y, Xiong JQ, Zhang WJ, Wang DS (2021) Correlation and mechanism of extracellular polymeric substances (EPS) on the effect of sewage sludge electro-dewatering. Sci Total Environ 801:149753. https://doi.org/10.1016/j.scitotenv.2021.149753
Zhao XQ, Jiao CJ, Su XL, Zhang JY, Ren BH (1991) Determination of DNA content in cells I. Modified diphenylamine method. Tianjin Med J 9:562–564 ((in Chinese))
Zhao K, Zhang YT, Lu ZL, Chen LZ, Zhang Y (2016) Effect of HS-SBR process on sedimentation and dewatering performance of activated sludge at low temperatures. China Water Wastewater 32:102–105 (in Chinese; CNKI:SUN:GSPS.0.2016-13-027)
Zhao W, Zhan XH, Liu WL, Wang S, Deng JY, Wei DY, Sun M (2021) Research on ultrasonic treatment in the field of actual excess sludge treatment. IOP Conf Ser 651:042046. https://doi.org/10.1088/1755-1315/651/4/042046
Zhou Y, Nguyen BT, Zhou C, Straka L, Lai YS, Xia SQ, Rittmann BE (2017) The distribution of phosphorus and its transformations during batch growth of Synechocystis. Water Res 122:355–362. https://doi.org/10.1016/j.watres.2017.06.017
Zhou LJ, Dong N, Ye B, Zhuang WQ, Xia SQ (2021) Assessing effects of Ca2+ addition on membrane bioreactor performance and macro-floc sludge characteristics. Sci Total Environ 798:149223. https://doi.org/10.1016/j.scitotenv.2021.149223
Zhu Z, Li T, Wang DS, Yao CH (2009) Role of Ca(II) in bioflocculation of activated sludge. Chinese J Environ Eng 3: 612–616 (in Chinese). http://159.226.240.226/handle/311016/5162
Zhuang XZ, Huang YQ, Liu HC, Yuan HY, Yin XL, Wu CZ (2018) Relationship between physicochemical properties and dewaterability of hydrothermal sludge derived from different source. J Environ Sci 69:261–270. https://doi.org/10.1016/j.jes.2017.10.021
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This work was supported by the National Natural Science Foundation of China (No.51478206).
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Zhao, K., Wang, J., Peng, G. et al. Influence of composite fillers on sludge characteristics and extracellular polymeric substance composition and structure in the sequencing batch reactor. Chem. Pap. 76, 4147–4158 (2022). https://doi.org/10.1007/s11696-022-02173-5
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DOI: https://doi.org/10.1007/s11696-022-02173-5