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Fabrication of Monarda citriodora essential oil nanoemulsions: characterization and antifungal activity against Penicillium digitatum of kinnow

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Abstract

Postharvest fungal pathogenic invasions are the major root cause of reduced shelf life of kinnow fruit, thereby contributing to the postharvest losses. Development of eco-friendly alternates are the need of the hour owing to health safety concerns for replacing the ongoing synthetic fungicide use. Essential oils with promising antimicrobial activities offer a promising solution but their hydrophobicity poses a big hindrance for exploiting the same. Present work was planned to explore their antimicrobial potential by developing their hydrophilic formulation with the use of nanotechnology. An in vitro study was conducted to assess the efficacy Monarda citriodora essential oil (MCEO) and its emulsions against major postharvest fungal pathogen of Kinnow; Penicillium digitatum. Both micro and nano formulations were prepared for different ratios of MCEO (0.5 to 3%) with different surfactant combinations and oil-surfactant-ratios (OSR) of 1:1 to 1:3. The influence of several process factors such as surfactant and oil phase concentrations, as well as sonication time intervals on emulsion stability was investigated by assessing attributes such as droplet diameter, Polydispersity index (PDI), zeta (ζ) potential and rheology. An emulsion formulated with 1% oil and 1:1 OSR treated with ultrasonic waves for 15 min was optimized with droplet diameter of 52.2 nm, 0.245 PDI and − 21 mV of ζ potential with consistent stability till 1 month. Further, in vitro antifungal activity of the optimized MCEO nanoemulsion exhibited the best efficacy with 100% inhibition at 200 mg L−1.

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Data availability

Data is available on request to the corresponding author.

References

  1. F. Peles, P. Sipos, S. Kovacs, Z. Gyori, I. Pocsi, T. Pusztahelyi, Toxins 13, 104 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. I. Saleh, R. Al-Thani, Vet World 12, 1877 (2019)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. W. Wang, S. Liu, L. Deng, J. Ming, S. Yao, K. Zeng, Front. Microbiol. 9, 2455 (2018)

    Article  PubMed  PubMed Central  Google Scholar 

  4. N. Paster, R. Barkai Golan, World Mycotoxin J. 1, 385 (2008)

    Article  CAS  Google Scholar 

  5. A.K. Chaudhari, V.K. Singh, S. Das, N.K. Dubey, Food Chem. Toxicol. 149, 112019 (2021)

    Article  CAS  PubMed  Google Scholar 

  6. S. Burt, Int. J. Food Microbiol. 94, 223 (2004)

    Article  CAS  PubMed  Google Scholar 

  7. J. Ju, X. Chen, Y. Xie, H. Yu, Y. Guo, Y. Cheng, W. Yao, Trends Food Sci. Technol. 92, 22 (2019)

    Article  CAS  Google Scholar 

  8. J.E. Collins, C.D. Bishop, S.G. Deans, K.P. Svoboda, J. Essent, Oil Res. 6, 27 (1994)

    Article  CAS  Google Scholar 

  9. A. Salama, R.M. Sabry, M.S. Eldin, Int. J. Pharm Tech. Res. 9, 67 (2016)

    CAS  Google Scholar 

  10. M. Fatima, M.A. Sheraz, S. Ahmed, S.H. Kazi, I. Ahmad, RADS J. Pharma Pharmaceut Sci. 2, 56 (2014)

    Google Scholar 

  11. T.J. Ashaolu, Environ. Chem. Lett. 19, 3381 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. J.L. Salager, Encyclopedia of emulsion technology (Marcel Dekker Inc, New York, 1988), p.80

    Google Scholar 

  13. T. Tadros, P. Izquiedo, J. Esquena, C. Solans, Adv. Colloid Interface Sci. 108–109, 303 (2004)

    Article  PubMed  Google Scholar 

  14. I.M. Geremias-Andrade, N.P. Souki, I.C. Moraes, S.C. Pinho, Gels 2, 22 (2016)

    Article  PubMed  PubMed Central  Google Scholar 

  15. R. Mahajan, R. Tandon, A. Kalia, B.V.C. Mahajan, J. Nanosci. Nanotech. 21, 3556 (2021)

    Article  Google Scholar 

  16. M.A. dos Santos, M. Roehrs, C.M. de Pereira, R.A. Freitag, A.V. de Bairros, Austin. Chromatogr. 14, 2014 (2014)

    Google Scholar 

  17. F. Mir, M.A. Rather, B.A. Dar, S. Rasool, A.S. Shawl, M.S. Alam, M.A. Qurishi, J. Pharm. Res. 5, 1353 (2012)

    Google Scholar 

  18. S. Belgheisi, A. Motamedzadegan, J.M. Milani, L. Rashidi, A. Rafe, J. Food Sc. Tech. Mys. 58, 484 (2020)

    Article  Google Scholar 

  19. A. Gupta, H.B. Eral, T.A. Hatton, P.S. Doyle, Soft Matter 12, 2826 (2016)

    Article  CAS  PubMed  Google Scholar 

  20. P.A. Rocha-Filho, M.F.P. Camargo, M. Ferrari, M. Maruno, J. Nanomed. Nanotech. 5, 2 (2014)

    Google Scholar 

  21. W.T. Chong, C.P. Tan, Y.K. Cheah, B.A.F. Lajis, N.L. Habi Mat Dian, S. Kanagaratnam, O.M. Lai, PLoS ONE 13, e0202771 (2018)

    Article  PubMed  PubMed Central  Google Scholar 

  22. M. Kamkar, S. Sadeghi, M. Arjmand, E. Aliabadian, U. Sundararaj, Nanomaterials 10, 1257 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. M. Balouiri, M. Sadiki, S.K. Ibnsouda, J. Pharm. Anal. 6, 71 (2016)

    Article  PubMed  Google Scholar 

  24. A.R. Wani, K. Yadav, Int. J. Pharm. Investig. 11, 170 (2021)

    Article  CAS  Google Scholar 

  25. Deepika, A. Singh, A.K. Chaudhari, S. Das, N.K. Dubey, J. Food Sci. Technol. 57, 2883 (2020)

    Article  Google Scholar 

  26. H.D. Dorman, S.G. Deans, J. Essen, Oil Res. 16, 145 (2004)

    Article  CAS  Google Scholar 

  27. L. Gontar, A. Herman, E. Osinska, Nat. Volatiles Essent. Oils. 8, 29 (2021)

    Article  CAS  Google Scholar 

  28. S.K. Lawson, P. Satyal, W.N. Setzer, Plants 10, 482 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. L.O. Orafidiya, F.A. Oladimeji, Int. J. Pharma. 237, 241 (2002)

    Article  CAS  Google Scholar 

  30. D.J. McClements, E.A. Decker, Y. Park, J. Weiss, Crit. Rev. Food Sci. Nutr. 49, 577 (2009)

    Article  CAS  PubMed  Google Scholar 

  31. D.J. McClements, “Emulsion stability” Food emulsions (CRC Press, Boca Raton, 2015), p.314

    Google Scholar 

  32. I.K. Hong, S.I. Kim, S.B. Lee, J. Ind. Eng. Chem. 67, 123 (2018)

    Article  CAS  Google Scholar 

  33. J.F. Scamehorn, Phenomena in mixed surfactant systems (American Chemical Society, Washington, 1986)

    Book  Google Scholar 

  34. V. Uskokovic, M. Drofenik, Surf. Rev. Lett. 12, 239 (2005)

    Article  CAS  Google Scholar 

  35. K. Ziani, Y. Fang, D.J. McClements, Food Chem. 134, 1106 (2012)

    Article  CAS  PubMed  Google Scholar 

  36. O. Sarheed, M. Dibi, K.V.R.N.S. Ramesh, Pharmaceutics 12, 1223 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. D. N. Do, D.P. Nguyen, H. D. Pham, T. A. Trieu, X. C. Luu Mater. Sci. Eng. 991, 012043 (IOP Publishing, 2020)

  38. J.C. Eriksson, S. Ljunggren, Langmuir 11, 1145 (1995)

    Article  CAS  Google Scholar 

  39. A.M. Hashtjin, S. Abbasi, Food Hydrocoll. 44, 40 (2015)

    Article  CAS  Google Scholar 

  40. S. Asadinezhad, F. Khodaiyan, M. Salami, H. Hosseini, B. Ghanbarzadeh, J. Food Meas. Charact. 13, 2501 (2019)

    Article  Google Scholar 

  41. S.G. Gaikwad, A.B. Pandit, Ultrason. Sonochem. 15, 554 (2008)

    Article  CAS  PubMed  Google Scholar 

  42. P.H. Campelo, L.A. Junqueira, J.V.D. Resende, R.D. Zacarias, R.V.D.B. Fernandes, D.A. Botrel, S.V. Borges, Int. J. Food Prop. 20, 564 (2017)

    Article  Google Scholar 

  43. N. Sharma, G. Kaur, S.K. Khatkar, LWT 141, 110962 (2021)

    Article  CAS  Google Scholar 

  44. Z. Wu, J. Wu, R. Zhang, S. Yuan, Q. Lu, Y. Yu, Carbohydr. Polym. 181, 56 (2018)

    Article  CAS  PubMed  Google Scholar 

  45. H.D. Silva, M.A. Cerqueira, A.A. Vicente, Food Bioproc. Tech. 5, 854 (2012)

    Article  CAS  Google Scholar 

  46. D.T. Piorkowski, D.J. McClements, Food Hydrocoll. 42, 5 (2014)

    Article  CAS  Google Scholar 

  47. N. Aligiannis, E. Kalpoutzakis, S. Mitaku, I.B. Chinou, J. Agric. Food Chem. 49, 4168 (2001)

    Article  CAS  PubMed  Google Scholar 

  48. M. Lis-Balchin, S.G. Deans, J. App. Micro. 82, 759 (1997)

    Article  CAS  Google Scholar 

  49. H. Lboutounne, J.F. Chaulet, C. Ploton, F. Falson, F. Pirot, J. Control Release 82, 319 (2002)

    Article  CAS  PubMed  Google Scholar 

  50. A.E. Hammad, M.M.H. Hassanin, Pak. J. Nematol. 40, 245 (2022)

    Google Scholar 

  51. M.K. Khan, B.A. Khan, B. Uzair, S.I. Niaz, H. Khan, K.M. Hosny, F. Menaa, Int J Nanomed. 16, 8203 (2021)

    Article  CAS  Google Scholar 

  52. M.J. Del Rio, C. Velez-Pardo, Archives Med. Res. 35, 185 (2004)

    Article  Google Scholar 

  53. N. Tao, L. Jia, H. Zhou, Food Chem. 153, 265 (2014)

    Article  CAS  PubMed  Google Scholar 

  54. T.S.T. Ariffin, E. Yahya, H. Husin, Procedia Eng. 148, 1149 (2016)

    Article  CAS  Google Scholar 

  55. M.I.D. Dg, V. Jideani, Acta Sci. Nutr Heal. 3, 105 (2019)

    Article  Google Scholar 

  56. L.R. Costa, A. Gomes, R.L. Cunha, Food Res. Int. 107, 717 (2018)

    Article  CAS  PubMed  Google Scholar 

  57. E.K. Silva, M.T.M. Gomes, M.D. Hubinger, R.L. Cunha, M.A.A. Meireles, Food Hydrocoll. 47, 1 (2015)

    Article  CAS  Google Scholar 

  58. L.G. Torres, R. Iturbe, M.J. Snowden, B.Z. Chowdhry, S.A. Leharne, Coll. Surf. 302, 439 (2007)

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to thank Dr. Sumit Singh, Anton Paar, India Ltd. for providing technical guidance to interpret rheological measurements.

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Authors and Affiliations

  1. Department of Chemistry, Punjab Agricultural University, Ludhiana, India

    Kiranjot Kaur & Ritu Tandon

  2. Department of Soil Science, Punjab Agricultural University, Ludhiana, India

    Anu Kalia

  3. Department of Chemistry, Central University of Punjab, Ghudda, Bathinda, India

    J. Nagendra Babu

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  1. Kiranjot Kaur
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  2. Ritu Tandon
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Correspondence to Ritu Tandon.

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Kaur, K., Tandon, R., Kalia, A. et al. Fabrication of Monarda citriodora essential oil nanoemulsions: characterization and antifungal activity against Penicillium digitatum of kinnow. Food Measure 17, 3044–3060 (2023). https://doi.org/10.1007/s11694-023-01821-y

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