Abstract
In the present study, the antimicrobial activity of kefir and probiotic yogurt produced from cow, camel, ewe, and goat milk on pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica) and fungi (Aspergillus niger, Penicillium sp., and Fusarium sp.) during 20-day storage period at 4 ºC was investigated. The carbohydrate content and pH of milk samples decreased during the fermentation process to produce probiotic yogurt and kefir, also the acidity increased significantly. The results revealed that kefir samples had stronger antifungal and antibacterial effect than probiotic yogurt samples. Among kefir samples, the ewe and cow milk kefir expressed the highest and the lowest antimicrobial activity, respectively. A. niger, S. aureus, and L. monocytogenes were the most sensitive and Penicillium sp. and E. coli were the most resistant microorganisms against treatment by kefir and probiotic yogurt.
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D. Dalié, A. Deschamps, F. Richard-Forget, Lactic acid bacteria–potential for control of mould growth and mycotoxins: a review. Food Control 21(4), 370–380 (2010)
P. Russo et al., Lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. Int. J. Food Microbiol. 247, 48–54 (2017)
E.M. EL-Fakharany et al., Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7. 5 hepatoma. Virol. J. 9(1), 201 (2012)
M.H. Yassin et al., Antimicrobial effects of camel milk against some bacterial pathogens. J. Food Nutr. Res. 3(3), 162–168 (2015)
F.O. Ebrahim et al., Purification of lactoferrin from camel colostrum and protein profiles of camel and bovine milk. Alex. J. Vet. Sci. 60(2), 67–70 (2019)
R. Omer, A. Eltinay, Microbial quality of camel’s raw milk in central & southern regions of United Arab Emirates. Emir. J. Food Agric. 20, 76–83 (2008)
M.S.F. de Lima et al., Brazilian Kefir-fermented sheep’s milk, a source of antimicrobial and antioxidant peptides. Probiotics Antimicrob. Proteins 10(3), 446–455 (2018)
C.G. Papadimitriou et al., Identification of peptides in traditional and probiotic sheep milk yoghurt with angiotensin I-converting enzyme (ACE)-inhibitory activity. Food Chem. 105(2), 647–656 (2007)
A.S. Akalın, Dairy-derived antimicrobial peptides: action mechanisms, pharmaceutical uses and production proposals. Trends Food Sci. Technol. 36(2), 79–95 (2014)
S. BoyCheVa et al., Quality characteristics of yogurt from goat’s milk, supplemented with fruit juice. Czech J. Food Sci. 29(1), 24–30 (2011)
F. Yangilar, As a potentially functional food: goats’ milk and products. J. Food Nutr. Res. 1(4), 68–81 (2013)
L. Yilmaz, T. Ozcan Yilsay, A. Akpinar, Bayizit, The sensory characteristics of berry-flavoured kefir. Czech J. Food Sci. 24(1), 26 (2006)
S. Cenesiz et al., The effect of kefir on glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels in mice with colonic abnormal crypt formation (ACF) induced by azoxymethane (AOM). Dtsch. Tierarztl. Wochenschr. 115(1), 15–19 (2008)
M.H. Namaei, M. Ghannadkafi, M. Ziaee, Antibacterial effect of non-industrial yogurt on Salmonella and Shigella. Modern Care J. 12(3), 109–113 (2015)
N. Said et al., Assessment of the antibacterial activity of goat milk kefir on Escherichia coli ATCC 8739 and Salmonella enteric subsp. enterica serovar typhimurium ATCC 14028 using a well diffusion method, in IOP Conference Series: Earth and Environmental Science. (IOP Publishing, Bristol, 2019)
C. Puerari, K.T. Magalhães, R.F. Schwan, New cocoa pulp-based kefir beverages: microbiological, chemical composition and sensory analysis. Food Res. Int. 48(2), 634–640 (2012)
L. Yilmaz-Ersan et al., The antioxidative capacity of kefir produced from goat milk. Int. J. Chem. Eng. Appl. 7(1), 22 (2016)
AOAC, Official of Analysis of the Association of Official Analytical Chemists. Method.15, Determination of Moisture Content, 2005; Method 942.05, Determination of Ash Content, 2000; Method 981.10, Determination of Protein Content, 2016; Method 2000.18, Determination of Milk Fat Content, 2001; Method 995.13, Determination of Carbohydrate Content, 2011; Method 942.15, Determination of Total (AOAC International, Washington, 2000)
D.-H. Kim et al., Growth inhibition of Cronobacter sakazakii in experimentally contaminated powdered infant formula by kefir supernatant. J. Food Prot. 78(9), 1651–1655 (2015)
F.B. Taheur, C. Mansour, K. Chaieb, Inhibitory effect of kefir on Aspergillus growth and mycotoxin production. Euro-Mediterr. J. Environ. Integr. 5(1), 5 (2020)
S.D. Eddine et al., Antifungal and antibacterial activity of some Lactobacilli isolated from camel’s milk biotope in the south of Algeria. J. Microbiol. Biotechnol. Food Sci. 9(4), 871–877 (2020)
R.R. Gamba et al., Antifungal activity against Aspergillus parasiticus of supernatants from whey permeates fermented with kéfir grains. Adv. Microbiol. 05(06), 479–492 (2015)
M. Cheesbrough, Medical Laboratory Manual for Tropical Countries: Microbiology (Linacre House, Jordan Hill, Oxford, 2000)
R.R. Gamba et al., Chemical, microbiological, and functional characterization of kefir produced from cow’s milk and soy milk. Int. J. Microbiol. 2020, 1–11 (2020)
C. Matar et al., Biologically active peptides released in fermented milk: role and functions, in Handbook of Fermented Functional Foods. (CRC Press, Boca Raton, 2003), pp. 193–218
D.-H. Kim et al., Antimicrobial activity of kefir against various food pathogens and spoilage bacteria. Korean J. Food Sci. Anim. Resour. 36(6), 787 (2016)
L. Yilmaz-Ersan et al., Comparison of antioxidant capacity of cow and ewe milk kefirs. J. Dairy Sci. 101(5), 3788–3798 (2018)
G. Satir, Z.B. Guzel-Seydim, Influence of Kefir fermentation on the bioactive substances of different breed goat milks. LWT-Food Sci. Technol. 63(2), 852–858 (2015)
W. Gao et al., Microbial diversity and stability during primary cultivation and subcultivation processes of Tibetan kefir. Int. J. Food Sci. Technol. 50(6), 1468–1476 (2015)
F. Montanuci, S. Garcia, S. Prudencio, Sensory characterization and acceptance of sweetened full fat and low fat kefir with inulin. Braz. J. Food Technol. 8, 79–90 (2010)
K.T. Magalhães et al., Brazilian kefir: structure, microbial communities and chemical composition. Braz. J. Microbiol. 42(2), 693–702 (2011)
M.G.C.P. Miguel et al., Diversity of bacteria present in milk kefir grains using culture-dependent and culture-independent methods. Food Res. Int. 43(5), 1523–1528 (2010)
A. Londero et al., Fermented whey as poultry feed additive to prevent fungal contamination. J. Sci. Food Agric. 94(15), 3189–3194 (2014)
C.A. Caro Velez, ÁM. Leon, Pelaez, Fungal growth inhibition of Aspergillus ochraceus with “Panela” fermented with water kefir grains. Vitae 21(3), 191–200 (2014)
A.A. Ismaiel, M.F. Ghaly, A.K. El-Naggar, Milk kefir: ultrastructure, antimicrobial activity and efficacy on aflatoxin B1 production by Aspergillus flavus. Curr. Microbiol. 62(5), 1602–1609 (2011)
R.R. Gamba et al., Application of whey permeate fermented with kefir grains for the shelf-life improvement of food and feed. Adv. Microbiol. 6, 650–661 (2016)
J. Miao et al., Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp. tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China. Food Control 42, 48–53 (2014)
K.L. Rodrigues et al., Antimicrobial and healing activity of kefir and kefiran extract. Int. J. Antimicrob. Agents 25(5), 404–408 (2005)
J. Van Wyk, The Inhibitory Activity and Sensory Properties of Kefir, Targeting the Low-Income African Consumer Market (Stellenbosch University, Stellenbosch, 2000)
M.C. Chifiriuc, A.B. Cioaca, V. Lazar, In vitro assay of the antimicrobial activity of kephir against bacterial and fungal strains. Anaerobe 17(6), 433–435 (2011)
A. Santos et al., The antimicrobial properties of different strains of Lactobacillus spp. isolated from kefir. Syst. Appl. Microbiol. 26(3), 434–437 (2003)
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Azizkhani, M., Saris, P.E.J. & Baniasadi, M. An in-vitro assessment of antifungal and antibacterial activity of cow, camel, ewe, and goat milk kefir and probiotic yogurt. Food Measure 15, 406–415 (2021). https://doi.org/10.1007/s11694-020-00645-4
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DOI: https://doi.org/10.1007/s11694-020-00645-4