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Spatial patterns nitrogen transfer models of ectomycorrhizal networks in a Mongolian scotch pine plantation

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Abstract

Ectomycorrhizal (EM) networks provide a variety of services to plants and ecosystems include nutrient uptake and transfer, seedling survival, internal cycling of nutrients, plant competition, and so on. To deeply their structure and function in ecosystems, we investigated the spatial patterns and nitrogen (N) transfer of EM networks using 15N labelling technique in a Mongolian scotch pine (Pinus sylvestris var. mongolica Litv.) plantation in Northeastern China. In August 2011, four plots (20 × 20 m) were set up in the plantation. 125 ml 5 at.% 0.15 mol/L 15NH 154 NO3 solution was injected into soil at the center of each plot. Before and 2, 6, 30 and 215 days after the 15N application, needles (current year) of each pine were sampled along four 12 m sampling lines. Needle total N and 15N concentrations were analyzed. We observed needle N and 15N concentrations increased significantly over time after 15N application, up to 31 and 0.42%, respectively. There was no correlation between needle N concentration and 15N/14N ratio (R2 = 0.40, n = 5, P = 0.156), while excess needle N concentration and excess needle 15N/14N ratio were positively correlated across different time intervals (R2 = 0.89, n = 4, P < 0.05), but deceased with time interval lengthening. Needle 15N/14N ratio increased with time, but it was not correlated with distance. Needle 15N/14N ratio was negative with distance before and 6th day and 30th day, positive with distance at 2nd day, but the trend was considerably weaker, their slop were close to zero. These results demonstrated that EM networks were ubiquitous and uniformly distributed in the Mongolian scotch pine plantation and a random network. We found N transfer efficiency was very high, absorbed N by EM network was transferred as wide as possible, we observed N uptake of plant had strong bias for 14N and 15N, namely N fractionation. Understanding the structure and function of EM networks in ecosystems may lead to a deeper understanding of ecological stability and evolution, and thus provide new theoretical approaches to improve conservation practices for the management of the Earth’s ecosystems.

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References

  • Albarracín MV, Six J, Houlton BZ, Bledsoe CS (2013) A nitrogen fertilization field study of carbon-13 and nitrogen-15 transfers in ectomycorrhizas of Pinus sabiniana. Oecologia 173:1439–1450

    Article  PubMed  Google Scholar 

  • Bahram M, Harend H, Tedersoo L (2014) Network perspectives of ectomycorrhizal associations. Fungal Ecol 7:70–77

    Article  Google Scholar 

  • Barto EK, Monika H, Frank M, Mohney BK, Weidenhamer JD, Rillig MC (2011) The fungal fast lane: common mycorrhizal networks extend bioactive zones of allelochemicals in soils. PLoS ONE 6:e27195

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Barto EK, Weidenhamer JD, Cipollini D, Rillig MC (2012) Fungal superhighways: do common mycorrhizal networks enhance below ground communication? Trends Plant Sci 17:633–637

    Article  CAS  PubMed  Google Scholar 

  • Beiler KJ, Durall DM, Simard SW, Maxwell SA, Kretzer AM (2010) Architecture of the wood-wide web: Rhizopogon spp. genets link multiple Douglas-fir cohorts. New Phytol 185:543–553

    Article  CAS  PubMed  Google Scholar 

  • Beiler KJ, Simard SW, Durall DM (2015) Topology of tree–mycorrhizal fungus interaction networks in xeric and mesic Douglas-fir forests. J Ecol 103:616–628

    Article  Google Scholar 

  • Bledsoe C, Allen MF, Southworth D (2014) Beyond Mutualism: Complex Mycorrhizal Interactions. In: Lüttge U, Beyschlag W, Cushman J (eds) Progress in Botany, vol 75. Springer, Berlin, pp 311–334

    Chapter  Google Scholar 

  • Booth MG (2004) Mycorrhizal networks mediate overstorey-understorey competition in a temperate forest. Ecol Lett 7:538–546

    Article  Google Scholar 

  • Brady NC, Weil RR (2002) The nature and properties of soils, 13th edn. Prentice Hall, Upper Saddle River

    Google Scholar 

  • Callesen I, Nilsson L, Schmidt I, Vesterdal L, Ambus P, Christiansen J, HögbergP Gundersen P (2013) The natural abundance of 15N in litter and soil profiles under six temperate tree species: N cycling depends on tree species traits and site fertility. Plant Soil 368:375–392

    Article  CAS  Google Scholar 

  • Cheng CX, Li J, Sun PF (2010) Analyses of the climate change tendency and abrupt climate change in Wuying, Xiaoxing’an Mountain in recent 49 years. Heilongjiang Meteotol 4:9–12

    Google Scholar 

  • Corrêa A, Strasser RJ, Martins-Loução MA (2008) Response of plants to ectomycorrhizae in N-limited conditions: which factors determine its variation? Mycorrhiza 18:413–427

    Article  PubMed  Google Scholar 

  • Dawson TE, Mambelli S, Plamboeck AH, Templer PH, Tu KP (2002) Stable isotopes in plant ecology. Annu Rev Ecol Syst 33:507–559

    Article  Google Scholar 

  • Fry B (2006) Stable isotope ecology. Springer, New York

    Book  Google Scholar 

  • He XH, Critchley C, Bledsoe CS (2003) Nitrogen transfer within and between plants through common mycorrhizal networks (CMNs). Crit Rev Plant Sci 22:531–567

    Article  Google Scholar 

  • He XH, Critchley C, Ng H, Bledsoe CS (2005) Nodulated N2-fixing Casuarina cunninghamiana is the sink for net N transfer from non-N2-fixing Eucalyptus maculata via an ectomycorrhizal fungus Pisolithus sp. using 15NH4 + or 15NO3 − supplied as ammonium nitrate. New Phytol 167:897–912

    Article  CAS  PubMed  Google Scholar 

  • He XH, Horwath WR, Zasoski RJ, Aanderud Z, Bledsoe CS (2007) Nitrogen sink strength of ectomycorrhizal morphotypes of Quercus douglasii, Q. garryana, and Q. agrifolia seedlings grown in a northern California oak woodland. Mycorrhiza 18:33–41

    Article  CAS  PubMed  Google Scholar 

  • Heaton LLM, López E, Maini PK, Fricker MD, Jones NS (2012) Advection, diffusion and delivery over a network. Phys Rev E Stat Nonlinear Soft Matter Phys 86:021905–021905

    Article  Google Scholar 

  • Heijden MGAVD, Horton TR (2009) Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems. J Ecol 97:1139–1150

    Article  Google Scholar 

  • Hobbie EA, Colpaert JV (2003) Nitrogen availability and colonization by mycorrhizal fungi correlate with nitrogen isotope patterns in plants. New Phytol 157:115–126

    Article  CAS  Google Scholar 

  • Hobbie E, Colpaert J, White M, Ouimette A, Macko S (2008) Nitrogen form, availability, and mycorrhizal colonization affect biomass and nitrogen isotope patterns in Pinus sylvestris. Plant Soil 310:121–136

    Article  CAS  Google Scholar 

  • Hobbie EA, van Diepen LTA, Lilleskov EA, Ouimette AP, Finzi AC, Hofmockel KS (2014) Fungal functioning in a pine forest: evidence from a 15N-labeled global change experiment. New Phytol 201:1431–1439

    Article  CAS  PubMed  Google Scholar 

  • Högberg P, Högberg MN, Quist ME, Ekblad ALF, Näsholm T (1999) Nitrogen isotope fractionation during nitrogen uptake by ectomycorrhizal and non-mycorrhizal Pinus sylvestris. New Phytol 142:569–576

    Article  Google Scholar 

  • Högberg P, Johannisson C, Yarwood S, Callesen I, Näsholm T, Myrold DD, Högberg MN (2011) Recovery of ectomycorrhiza after ‘nitrogen saturation’ of a conifer forest. New Phytol 189:515–525

    Article  PubMed  Google Scholar 

  • Knowles RR, Blackburn TH (1993) Nitrogen isotope techniques. Academic Press, San Diego

    Google Scholar 

  • Koide RT, Fernandez C, Malcolm G (2014) Determining place and process: functional traits of ectomycorrhizal fungi that affect both community structure and ecosystem function. New Phytol 201:433–439

    Article  PubMed  Google Scholar 

  • Kranabetter JM, Hawkins BJ, Jones MD, Robbins S, Dyer T, Li T (2015) Species turnover (β-diversity) in ectomycorrhizal fungi linked to NH4 + uptake capacity. Mol Ecol 24:5992–6005

    Article  CAS  PubMed  Google Scholar 

  • Lang C, Finkeldey R, Polle A (2013) Spatial patterns of ectomycorrhizal assemblages in a monospecific forest in relation to host tree genotype. Front Plant Sci 4:103

    Article  PubMed  PubMed Central  Google Scholar 

  • Leake JR, Johnson D, Donnelly DP, Muckle GE, Boddy L, Read DJ (2004) Networks of power and influence: the role of mycorrhizal mycelium in controlling plant communities and agroecosystem functioning. Can J Bot 82:1016–1045

    Article  Google Scholar 

  • Mayor J, Schuur EG, Mack M, Hollingsworth T, Bååth E (2012) Nitrogen isotope patterns in Alaskan black spruce reflect organic nitrogen sources and the activity of ectomycorrhizal fungi. Ecosystems 15:819–831

    Article  CAS  Google Scholar 

  • Mayor JR, Mack MC, Schuur EAG (2015a) Decoupled stoichiometric, isotopic, and fungal responses of an ectomycorrhizal black spruce forest to nitrogen and phosphorus additions. Soil Biol Biochem 88:247–256

    Article  CAS  Google Scholar 

  • Mayor J, Bahram M, Henkel T, Buegger F, Pritsch K, Tedersoo L (2015b) Ectomycorrhizal impacts on plant nitrogen nutrition: emerging isotopic patterns, latitudinal variation and hidden mechanisms. Ecol Lett 18:96–107

    Article  PubMed  Google Scholar 

  • McGuire KL (2007) Common ectomycorrhizal networks may maintain monodominance in a tropical rain forest. Ecology 88:567–574

    Article  PubMed  Google Scholar 

  • Moyer-Henry KA, Burton JW, Israel DW, Rufty TW (2006) Nitrogen transfer between plants: A 15N natural abundance study with crop and weed species. Plant Soil 282:7–20

    Article  CAS  Google Scholar 

  • Nara K (2006) Ectomycorrhizal networks and seedling establishment during early primary succession. New Phytol 169:169–178

    Article  CAS  PubMed  Google Scholar 

  • Nave LE, Nadelhoffer KJ, Moine JM, Diepen LTA, Cooch JK, Dyke NJ (2013) Nitrogen uptake by trees and mycorrhizal fungi in a successional northern temperate forest: insights from multiple isotopic methods. Ecosystems 16:590–603

    Article  CAS  Google Scholar 

  • Pena R, Polle A (2014) Attributing functions to ectomycorrhizal fungal identities in assemblages for nitrogen acquisition under stress. ISME J 8:321–330

    Article  CAS  PubMed  Google Scholar 

  • Pickles BJ, Genney DR, Anderson IC, Alexander IJ (2012) Spatial analysis of ectomycorrhizal fungi reveals that root tip communities are structured by competitive interactions. Mol Ecol 21:5110–5123

    Article  PubMed  Google Scholar 

  • Selosse MA, Richard F, He XH, Simard SW (2006) Mycorrhizal networks: des liaisons dangereuses? Trends Ecol Evol 21:621–628

    Article  PubMed  Google Scholar 

  • Simard SW, Beiler KJ, Bingham MA, Deslippe JR, Philip LJ, Teste FP (2012) Mycorrhizal networks: mechanisms, ecology and modelling. Fungal Biol Rev 26:39–60

    Article  Google Scholar 

  • Simard S, Asay A, Beiler K, Bingham M, Deslippe J, He XH, Philip L, Song YY, Teste F (2015) Resource transfer between plants through ectomycorrhizal fungal networks. In: Horton RT (ed) Mycorrhizal networks. Springer, Dordrecht, pp 133–176

    Chapter  Google Scholar 

  • Southworth D, He XH, Swenson W, Bledsoe CS, Horwath WR (2005) Application of network theory to potential mycorrhizal networks. Mycorrhiza 15:589–595

    Article  CAS  PubMed  Google Scholar 

  • Teste FP, Simard SW, Durall DM, Guy RD, Jones MD, Schoonmaker AL (2009) Access to mycorrhizal networks and roots of trees: importance for seedling survival and resource transfer. Ecology 90:2808–2822

    Article  PubMed  Google Scholar 

  • Toju H, Yamamoto S, Tanabe AS, Hayakawa T, Ishii HS (2016) Network modules and hubs in plant-root fungal biomes. J R Soc Interface 13(116):20151097. doi:10.1098/rsif.2015.1097

    Article  PubMed  PubMed Central  Google Scholar 

  • Wu BY, Nara K, Hogetsu T (2002) Spatiotemporal transfer of carbon-14-labelled photosynthate from ectomycorrhizal Pinus densiflora seedlings to extraradical mycelia. Mycorrhiza 12:83–88

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. College of Life Science, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Yanbin Liu, Hongmei Chen & Pu Mou

  2. Institute of Forestry Science, Heilongjiang Academy of Forestry, Harbin, 150081, People’s Republic of China

    Yanbin Liu

  3. Institute of Biology, University of Leipzig, Johannisallee 21, 04103, Leipzig, Germany

    Hongmei Chen

  4. State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing, 100875, People’s Republic of China

    Pu Mou

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  1. Yanbin Liu
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  2. Hongmei Chen
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  3. Pu Mou
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Correspondence to Yanbin Liu.

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Project funding: This work was supported by National Natural Science Foundation of China (30830024).

The online version is available at http://www.springerlink.com

Corresponding editor: Tao Xu.

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Liu, Y., Chen, H. & Mou, P. Spatial patterns nitrogen transfer models of ectomycorrhizal networks in a Mongolian scotch pine plantation. J. For. Res. 29, 339–346 (2018). https://doi.org/10.1007/s11676-017-0454-z

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  • DOI: https://doi.org/10.1007/s11676-017-0454-z

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