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Molecular approach to determine taxonomic status of Septoria sp. causing leaf blotch of Castanea sativa in Hyrcanian forests

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Abstract

Castanea sativa is a valuable tree species in Hyrcanian forests, an evolutionary relict ecosystem whose communities suffer from overexploitation and fungal diseases. In the current study, three species delimitation methods were utilized with ITS regions sequencing to determine the taxonomic status of Septoria causing leaf blotch of C. sativa in Hyrcanian forests. The results indicated that the length of ITS region in the genus Septoria (extracted from GenBank) varied from 650 to 680 bp. There were almost three times more variable sites in ITS1 than in ITS2. The ITS2 secondary structure of Hyrcanian Septoria community had the highest similarity with Septoria castaneicola, except for some differences in helix II and III. Also, Hyrcanian samples had minimum genetic distances with S. castaneicola and maximum with Septoria quercicola. The maximum parsimony method divided the studied Septoria genus into three distinct clades, mostly located in clade I. Clade II consisted entirely of Septoria aciculosa, while clade III contained S. castaneicola as well as Hyrcanian samples.

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Acknowledgements

The authors appreciate the financial support of the Tarbiat Modares University for this research and are grateful to Fatemeh Akbarzadeh for her kind support in providing the molecular data of this paper.

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Authors and Affiliations

  1. Department of Forestry, Faculty of Natural Resources, Tarbiat Modares University, Tehran, Iran

    Hamed Yousefzadeh

  2. Biotechnology Department, Faculty of Energy Engineering and New Technology, Shahid Beheshti University, Tehran, Iran

    Abbas Saidi & Somayeh Tayebi

  3. Department of Arid Land Forestry, Faculty of Desert Studies, Semnan University, Semnan, 35196-45399, Iran

    Davoud Kartoolinejad

  4. Department of Engineering of Wood and Paper Industries, Faculty of Natural Resources, Semnan University, Semnan, Iran

    Reza Naghdi

Authors
  1. Hamed Yousefzadeh
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  2. Abbas Saidi
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  3. Somayeh Tayebi
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  4. Davoud Kartoolinejad
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  5. Reza Naghdi
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Corresponding author

Correspondence to Davoud Kartoolinejad.

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Project funding: This research was financially supported by Tarbiat Modares University.

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Corresponding editor: Chai Ruihai

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Yousefzadeh, H., Saidi, A., Tayebi, S. et al. Molecular approach to determine taxonomic status of Septoria sp. causing leaf blotch of Castanea sativa in Hyrcanian forests. J. For. Res. 28, 661–670 (2017). https://doi.org/10.1007/s11676-016-0363-6

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  • DOI: https://doi.org/10.1007/s11676-016-0363-6

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