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Analysis of EST-SSRs in silver birch (Betula pendula Roth.)

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Abstract

Simple sequence repeats (SSRs) defined as sequence repeat units between 1 and 6 bp occur abundantly in both coding and non-coding regions in eukaryotic genomes and these repeats can affect gene expression. In this study, ESTs (expressed sequence tags) of Betula pendula (silver birch) were analyzed for in silico mining of EST-SSRs, protein annotation, open reading frames (ORFs), designing primers, and identifying codon repetitions. In B. pendula, the frequency of ESTs containing SSRs was 7.8 % with an average of 1SSR/4. 78 kb of EST sequences. A total of 188 SSRs was identified by using MISA software and di-nucleotide SSR motifs (65.9 %) were found to be the most abundant type of repeat motif followed by tri- (27.1 %), tetra- (4.8 %), and penta- (2.2 %) motifs. Based on ORF analysis, 175 of 178 sequences were predicted as ORFs and the most frequent SSRs were detected in 5′ UTR (58.43 %), followed by in ORF (31.46 %) and in 3′ UTR (8.43 %). 102 of 178 ESTs were annotated as ribosomal protein, transport protein, membrane protein, carrier protein, binding protein, and transferase protein. For a total of 102 SSRs (57.3 %) with significant matches, a set of 102 primers (100 %) with forward and reverse strands was designed by using Primer3 software. Serine (Ser, 19.6 %) was predominant in putative encoded amino acids and most of amino acids showed nonpolar (35.3 %) nature. Our data provide resources for B. pendula and can be useful for in silico comparative analyses of Betulaceae species, including SSR mining.

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Authors and Affiliations

  1. Department of Crop and Animal Production, Cilimli Vocational School, Duzce University, Cilimli, 81750, Duzce, Turkey

    Ertugrul Filiz

  2. Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, 35430, Izmir, Turkey

    Ilhan Dogan

  3. Department of Biology, Faculty of Science, Rahatlayadururken Manas University, 72001, Bishkek, Kyrgystan

    Ilhan Dogan

  4. Department of Biology, Faculty of Science and Arts, Marmara University, Goztepe, 34722, Istanbul, Turkey

    Ibrahim Ilker Ozyigit

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  1. Ertugrul Filiz
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  2. Ilhan Dogan
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  3. Ibrahim Ilker Ozyigit
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Correspondence to Ertugrul Filiz.

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The online version is available at http://www.springerlink.com

Corresponding editor: Hu Yanbo

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Filiz, E., Dogan, I. & Ozyigit, I.I. Analysis of EST-SSRs in silver birch (Betula pendula Roth.). J. For. Res. 27, 639–646 (2016). https://doi.org/10.1007/s11676-015-0182-1

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  • DOI: https://doi.org/10.1007/s11676-015-0182-1

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