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Purification and structural analysis of the toxin AP-I from the pathogen of Bambusa pervariabilis × Dendrocalamopsis grandis blight

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Abstract

Bambusa pervariabilis × Dendrocalamopsis grandis blight is caused by a toxin from the fungus Arthrinium phaeospermum (corda) M. B. Ellis. We used shaking culture in a modified Fries culture medium and methanol extraction to isolate the toxin. The optimal developing solvent mixture (methanol: ethyl acetate: H2O at 7:1.5:3) was selected using thin layer chromatography and used as the eluent for toxin purification by silica gel column chromatography. Two toxic fractions were identified in the bioassay. A flaxen oil substance, AP-I, showed higher toxicity than a toxic white powder, AP-II. The more toxic AP-I was determined to be dibutyl phthalate (C16H22O4, molecular weight of 278) by mass spectrometry, nuclear magnetic resonance, and infrared spectrophotometry. Dibutyl phthalate might contribute to the pathogenesis of bamboo blight.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31070578) and the National Natural Science and Technology Resources Sharing Platform of China (2005DKA21207-13).

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Authors and Affiliations

  1. College of Forestry, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Shujiang Li, Tianhui Zhu, Tianmin Qiao & Shan Han

  2. Key Laboratory of Forest Protection of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Tianhui Zhu

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  1. Shujiang Li
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  2. Tianhui Zhu
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  3. Tianmin Qiao
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  4. Shan Han
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Correspondence to Tianhui Zhu.

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Project founding: This research was supported by the National Natural Science Foundation of China (31070578) and the National Natural Science and Technology Resources Sharing Platform of China (2005DKA21207-13).

The online version is available at http://www.springerlink.com

Corresponding editor: Chai Ruihai

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Li, S., Zhu, T., Qiao, T. et al. Purification and structural analysis of the toxin AP-I from the pathogen of Bambusa pervariabilis × Dendrocalamopsis grandis blight. J. For. Res. 26, 1035–1042 (2015). https://doi.org/10.1007/s11676-015-0129-6

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