Abstract
Objective: To investigate the expression of matrix metalloproteinase-9 (MMP-9) gene in cancer cells induced by adhesion with fibronectin and the underlying mechanism of cell invasion. Methods: Following adhesion of ovarian cancer cells A2780 to fibronectin, MMP mRNA expression was assayed by using reverse transcription-polymerase chain reaction (RT-PCR). MMP-9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells. Results: Adhesion could induce the expression of MMP-9 gene in A2780 cells, but did not affect longer the expression of MMP-2 or TIMP-1 gene. The induction was enhanced with longer adhesion time. When the transfected cells were allowed to adhere and spread on FN-coated surface, the promoter activity of MMP-9 gene was also enhanced dramatically. Conclusion: adhesion of cells with ECM may stimulate the expression of MMP-9 gene through stimulating the promoter activity, thereby enhancing cancer cell invasion and metastasis.
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Biography: Tian Fang(1958–), female, doctor of medicine, associate professor, Department of Tumor Molecular Biology, Institute of Basic Medical Sciences, Beijing, majors in tumor immunology
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Tian, F., Yan, Ch., Xue, H. et al. Adhesion induces matrix metalloproteinase-9 gene expression in ovarian cancer cells. Chin. J. Cancer Res. 14, 251–253 (2002). https://doi.org/10.1007/s11670-002-0055-y
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DOI: https://doi.org/10.1007/s11670-002-0055-y