Abstract
Objective: To establish a fluoregenic probe quantitative RT-PCR (FQ-RT-PCR) method for detection of the expression of MDR1 gene in tumor cells and to investigate the expression of MDR1 gene in patients with lung cancer. Methods: The fluorogenic quantitative RT-PCR method for detection of the expression of MDR1 gene was established. K562/ADM and K562 cell lines or 45 tumor tissues from patients with lung cancer were examined on PE Applied Biosystems 7700 Sequence Detection machine. Results: the average levels of MDR1 gene expression in K562/ADM cells and K562 cells were (6.86±0.65)× 107 copies/µg RNA and (8.49±0.67)×105 copies/µg RNA, respectively. The former was 80.8 times greater than the latter. Each sample was measured 10 times and the coefficient variation (CV) was 9.5% and 7.9%, respectively. Various levels of MDR1 gene expression were detected in 12 of 45 patients with lung cancer. Conclusion: Quantitative detection of MDR1 gene expression in tumor cells was achieved by using FQ-RT-PCR. FQ-RT-PCR is an accurate, and sensitive method and easy to perform. Using this method, low levels of MDR1 gene expression could be detected in 24% of the patients with lung cancer.
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Foundation item: This work was supported by a grant from the National New Technology Program (No. 1998-345).
Biography: Gao Jin-song, (1963–), doctor of medicine, Sun Yat-sen University of Medical Sciences, majors in gene diagnosis of malignant tumor.
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Gao, Js., Ma, G., Tong, M. et al. The detection of MDR1 gene expression using fluorogenic probe quantitative RT-PCR method. Chin. J. Cancer Res. 13, 96–100 (2001). https://doi.org/10.1007/s11670-001-0023-y
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DOI: https://doi.org/10.1007/s11670-001-0023-y