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The vitamin D receptor expression in skeletal muscle of women with distal radius fracture

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Abstract

Summary

We evaluated the vitamin D receptor (VDR) expression in the forearm flexor muscle of women with distal radius fracture. High VDR expression was associated with low appendicular lean mass index.

Introduction

We aimed to evaluate the relationship between the VDR expression in the muscle cell and the muscle mass in women with a distal radius fracture (DRF).

Methods

We prospectively recruited 45 women over 50 years of age (mean age, 66 years) with DRF and acquired biopsy of the forearm flexor muscle. The muscle cross-sectional area (CSA) and VDR expression were measured using immunohistochemistry staining. The clinical parameters including grip strength, gait speed, body mass index (BMI), bone mineral density (BMD), and serum vitamin D levels were compared between patients grouped by appendicular lean mass index and were correlated with the VDR expression.

Results

Twelve patients (27%) showed a decreased appendicular lean mass index, less than the cut-off value of 5.4 kg/m2 which was suggested by the Asian Working Group for Sarcopenia. Patients with a low appendicular lean mass index had significantly lower muscle CSA (p = 0.037), but a higher VDR expression (p = 0.045) than those with higher indices. VDR expression was negatively correlated with BMI (r = − 0.417, p = 0.004) and appendicular lean mass index (r = − 0.316, p = 0.044).

Conclusions

DRF patients with low appendicular lean mass index presented high VDR expression and low CSA in forearm muscle cells. This suggests that the VDR expression might be upregulated in the attempt to compensate for the decreasing muscle mass. Further studies are necessary to explore the role of VDR in the progression of sarcopenia.

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References

  1. Janssen I, Heymsfield SB, Wang Z, Ross R (2000) Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr. J Appl Physiol 89(1):81–88

    Article  CAS  Google Scholar 

  2. Lukaski HC (1996) Estimation of muscle mass; in Roche AF, Heymsfield SB, Lohman TG(eds): Human body composition. Human Kinetics, Champaign, pp 109–128

  3. Lexell J, Taylor CC, Sjöström M (1988) What is the cause of the ageing atrophy?: total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15-to 83-year-old men. J Neurol Sci 84(2):275–294

    Article  CAS  Google Scholar 

  4. Frontera WR, Hughes VA, Fielding RA, Fiatarone MA, Evans WJ, Roubenoff R (2000) Aging of skeletal muscle: a 12-yr longitudinal study. J Appl Physiol 88(4):1321–1326

    Article  CAS  Google Scholar 

  5. Lang T, Streeper T, Cawthon P, Baldwin K, Taaffe DR, Harris TB (2010) Sarcopenia: etiology, clinical consequences, intervention, and assessment. Osteoporos Int 21(4):543–559. https://doi.org/10.1007/s00198-009-1059-y

    Article  CAS  PubMed  Google Scholar 

  6. O'neill T, Marsden D, Adams J, Silman A (1996) Risk factors, falls, and fracture of the distal forearm in Manchester, UK. J Epidemiol Community Health 50(3):288–292

    Article  CAS  Google Scholar 

  7. Mallmin H, Ljunghall S (1994) Distal radius fracture is an early sign of general osteoporosis: bone mass measurements in a population-based study. Osteoporos Int 4(6):357–361. https://doi.org/10.1007/bf01622198

    Article  CAS  PubMed  Google Scholar 

  8. Jang WY, Chung MS, Baek GH, Song CH, Cho HE, Gong HS (2012) Vitamin D levels in post-menopausal Korean women with a distal radius fracture. Injury 43(2):237–241. https://doi.org/10.1016/j.injury.2011.10.020

    Article  PubMed  Google Scholar 

  9. Cho YJ, Gong HS, Song CH, Lee YH, Baek GH (2014) Evaluation of physical performance level as a fall risk factor in women with a distal radial fracture. J Bone Joint Surg Am 96(5):361–365. https://doi.org/10.2106/jbjs.l.01359

    Article  PubMed  Google Scholar 

  10. Roh YH, Koh YD, Noh JH, Gong HS, Baek GH (2017) Evaluation of sarcopenia in patients with distal radius fractures. Arch Osteoporos 12(1):5. https://doi.org/10.1007/s11657-016-0303-2

    Article  PubMed  Google Scholar 

  11. Owen R, MELTON III L, Ilstrup D, Johnson K, Riggs B (1982) Colles’ fracture and subsequent hip fracture risk. Clin Orthop Relat Res (171):37-43

  12. Visser M, Deeg DJ, Lips P, Longitudinal Aging Study A (2003) Low vitamin D and high parathyroid hormone levels as determinants of loss of muscle strength and muscle mass (sarcopenia): the Longitudinal Aging Study Amsterdam. J Clin Endocrinol Metab 88(12):5766–5772. https://doi.org/10.1210/jc.2003-030604

    Article  CAS  PubMed  Google Scholar 

  13. Snijder MB, van Schoor NM, Pluijm SM, van Dam RM, Visser M, Lips P (2006) Vitamin D status in relation to one-year risk of recurrent falling in older men and women. J Clin Endocrinol Metab 91(8):2980–2985. https://doi.org/10.1210/jc.2006-0510

    Article  CAS  PubMed  Google Scholar 

  14. Ceglia L, Harris SS (2013) Vitamin D and its role in skeletal muscle. Calcif Tissue Int 92(2):151–162. https://doi.org/10.1007/s00223-012-9645-y

    Article  CAS  PubMed  Google Scholar 

  15. Simpson RU, Thomas GA, Arnold AJ (1985) Identification of 1,25 dihydroxyvitamin D3 receptors and activities in muscle. J Biol Chem 260(July 25):8882–8891

    CAS  PubMed  Google Scholar 

  16. Bischoff HA, Borchers M, Gudat F, Duermueller U, Theiler R, HB S¨a, Dick W (2001) In situ detection of 1,25dihydroxycitamin D3 receptor in human skeletal muscle tissue. Histochem J 33(1):19–24

    Article  CAS  Google Scholar 

  17. Girgis CM, Clifton-Bligh RJ, Hamrick MW, Holick MF, Gunton JE (2013) The roles of vitamin D in skeletal muscle: form, function, and metabolism. Endocr Rev 34(1):33–83. https://doi.org/10.1210/er.2012-1012

    Article  CAS  PubMed  Google Scholar 

  18. Endo I, Inoue D, Mitsui T, Umaki Y, Akaike M, Yoshizawa T, Kato S, Matsumoto T (2003) Deletion of vitamin D receptor gene in mice results in abnormal skeletal muscle development with deregulated expression of myoregulatory transcription factors. Endocrinology 144(12):5138–5144

    Article  CAS  Google Scholar 

  19. Chen LK, Liu LK, Woo J, Assantachai P, Auyeung TW, Bahyah KS, Chou MY, Chen LY, Hsu PS, Krairit O (2014) Sarcopenia in Asia: consensus report of the Asian Working Group for Sarcopenia. J Am Med Dir Assoc 15(2):95–101

    Article  Google Scholar 

  20. Petersen P, Petrick M, Connor H, Conklin D (1989) Grip strength and hand dominance: challenging the 10% rule. Am J Occup Ther 43(7):444–447

    Article  CAS  Google Scholar 

  21. Wang D, Li T, Ye G, Shen Z, Hu Y, Mou T, Yu J, Li S, Liu H, Li G (2015) Overexpression of the receptor for advanced glycation endproducts (RAGE) is associated with poor prognosis in gastric cancer. PLoS One 10(4):e0122697. https://doi.org/10.1371/journal.pone.0122697

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Remmele W, Hildebrand U, Hienz HA, Klein P-J, Vierbuchen M, Behnken LJ, Heicke B, Scheidt E (1986) Comparative histological, histochemical, immunohistochemical and biochemical studies on oestrogen receptors, lectin receptors, and Barr bodies in human breast cancer. Virchows Archiv A 409(2):127–147

    Article  CAS  Google Scholar 

  23. Ceglia L, Niramitmahapanya S, Price LL, Harris SS, Fielding RA, Dawson-Hughes B (2013) An evaluation of the reliability of muscle fiber cross-sectional area and fiber number measurements in rat skeletal muscle. Biol Proced Online 15(1):6

    Article  CAS  Google Scholar 

  24. Ceglia L, Niramitmahapanya S, da Silva Morais M, Rivas DA, Harris SS, Bischoff-Ferrari H, Fielding RA, Dawson-Hughes B (2013) A randomized study on the effect of vitamin D3supplementation on skeletal muscle morphology and vitamin D receptor concentration in older women. J Clin Endocrinol Metab 98(12):E1927–E1935. https://doi.org/10.1210/jc.2013-2820

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Cruz-Jentoft AJ, Landi F, Schneider SM, Zúñiga C, Arai H, Boirie Y, Chen L-K, Fielding RA, Martin FC, Michel J-P (2014) Prevalence of and interventions for sarcopenia in ageing adults: a systematic review. Report of the International Sarcopenia Initiative (EWGSOP and IWGS). Age Ageing 43(6):748–759

    Article  Google Scholar 

  26. Kwon HJ, Ha YC, Park HM (2016) Prevalence of sarcopenia in the Korean woman based on the Korean National Health and Nutritional Examination Surveys. J Bone Metab 23(1):23–26

    Article  Google Scholar 

  27. Silman A (2003) Risk factors for Colles' fracture in men and women: results from the European Prospective Osteoporosis Study. Osteoporos Int 14(3):213–218

    Article  CAS  Google Scholar 

  28. Kelsey JL, Prill MM, Keegan TH, Tanner HE, Bernstein AL, Quesenberry CP, Sidney S (2005) Reducing the risk for distal forearm fracture: preserve bone mass, slow down, and don’t fall! Osteoporos Int 16(6):681–690

    Article  Google Scholar 

  29. Simpson R, Thomas G, Arnold A (1985) Identification of 1, 25-dihydroxyvitamin D3 receptors and activities in muscle. J Biol Chem 260(15):8882–8891

    CAS  PubMed  Google Scholar 

  30. Horst R, Goff J, Reinhardt T (1990) Advancing age results in reduction of intestinal and bone 1,25-dihydroxyvitamin D receptor. Endocrinology 126(2):1053–1057

    Article  CAS  Google Scholar 

  31. Bischoff-Ferrari H, Borchers M, Gudat F, Dürmüller U, Stähelin H, Dick W (2004) Vitamin D receptor expression in human muscle tissue decreases with age. J Bone Miner Res 19(2):265–269

    Article  CAS  Google Scholar 

  32. Kinyamu HK, Gallagher JC, Prahl JM, Deluca HF, Petranick KM, Lanspa SJ (1997) Association between intestinal vitamin D receptor, calcium absorption, and serum 1,25 dihydroxyvitamin D in normal young and elderly women. J Bone Miner Res 12(6):922–928. https://doi.org/10.1359/jbmr.1997.12.6.922

    Article  CAS  PubMed  Google Scholar 

  33. Pojednic RM, Ceglia L, Olsson K, Gustafsson T, Lichtenstein AH, Dawson-Hughes B, Fielding RA (2015) Effects of 1,25-dihydroxyvitamin D3 and vitamin D3 on the expression of the vitamin d receptor in human skeletal muscle cells. Calcif Tissue Int 96(3):256–263. https://doi.org/10.1007/s00223-014-9932-x

    Article  CAS  PubMed  Google Scholar 

  34. Bozsodi A, Boja S, Szilagyi A, Somhegyi A, Varga PP, Lazary A (2016) Muscle strength is associated with vitamin D receptor gene variants. J Orthop Res 34(11):2031–2037. https://doi.org/10.1002/jor.23220

    Article  CAS  PubMed  Google Scholar 

  35. Girgis CM, Cha KM, Houweling PJ, Rao R, Mokbel N, Lin M, Clifton-Bligh RJ, Gunton JE (2015) Vitamin D receptor ablation and vitamin D deficiency result in reduced grip strength, altered muscle fibers, and increased myostatin in mice. Calcif Tissue Int 97(6):602–610. https://doi.org/10.1007/s00223-015-0054-x

    Article  CAS  PubMed  Google Scholar 

  36. Garcia LA, King KK, Ferrini MG, Norris KC, Artaza JN (2011) 1, 25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152(8):2976–2986

    Article  CAS  Google Scholar 

  37. Perez-Siles G, Grant A, Ellis M, Ly C, Kidambi A, Khalil M, Llanos RM, La Fontaine S, Strickland AV, Züchner S (2016) Characterizing the molecular phenotype of an Atp7a T985I conditional knock in mouse model for X-linked distal hereditary motor neuropathy (dHMNX). Metallomics 8(9):981–992

    Article  CAS  Google Scholar 

  38. Frontera WR, Reid KF, Phillips EM, Krivickas LS, Hughes VA, Roubenoff R, Fielding RA (2008) Muscle fiber size and function in elderly humans: a longitudinal study. J Appl Physiol (1985) 105(2):637–642. https://doi.org/10.1152/japplphysiol.90332.2008

    Article  Google Scholar 

  39. Brennan-Speranza TC, Mor D, Mason RS, Bartlett JR, Duque G, Levinger I, Levinger P (2017) Skeletal muscle vitamin D in patients with end stage osteoarthritis of the knee. J Steroid Biochem Mol Biol 173:180–184. https://doi.org/10.1016/j.jsbmb.2017.01.022

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by a research fund (2015R1D1A1A01058562) from the National Research Foundation of Korea and in part by a basic research fund (14-2015-001) from the authors’ institution.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Hallym University College of Medicine, Chuncheon, South Korea

    Kahyun Kim

  2. Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, South Korea

    Hyun Sik Gong & Goo Hyun Baek

  3. Department of Orthopaedic Surgery, Seoul National University Bundang Hospital, 300 Gumidong, Bundanggu, Seongnam, Gyeonggido, 463-707, Republic of Korea

    Hyun Sik Gong

  4. Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul, South Korea

    Jae-Young Lim

  5. Department of Physical Education, Hanyang University, Seoul, South Korea

    Jong Hee Kim

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Correspondence to Hyun Sik Gong.

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The Seoul National University Bundang Hospital Institutional Review Board (SNUBH IRB) reviewed the protocol and approved the study (B-1501-282-002). All participants provided informed written consents.

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Kim, K., Gong, H.S., Lim, JY. et al. The vitamin D receptor expression in skeletal muscle of women with distal radius fracture. Arch Osteoporos 13, 24 (2018). https://doi.org/10.1007/s11657-018-0442-8

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