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Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes

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Objective

To study the effect of salvianolic acid A (SAA) on L-type calcium current (I-CaL) in isolated ventricular myocytes of Sprague-Dawley rats.

Methods

SAA powder was dissolved in normal Tyrode’s solution to reach the concentrations of 1, 10, 100, and 1000 μmol/L. The traditional whole-cell patch-clamp recording technique was employed to evaluate the effects of SAA on I-CaL in single ventricular myocytes which were prepared by Langendorff perfusion apparatus from Sprague-Dawley rats.

Results

SAA (1, 10, 100, and 1000 μmol/L) inhibited I-CaL peak value by 16.23%±1.3% (n=6, P<0.05), 22.9%±3.6% (n=6, P<0.05), 53.4%±3.0% (n=8, P<0.01), and 62.26%±2.9% (n=6, P<0.01), respectively. SAA reversibly inhibited I-CaL in a dose-dependent manner and with a half-blocking concentration (IC50) of 38.3 μmol/L. SAA at 100 μmol/L elevated the I-V curve obviously, and shifted the half-active voltage (V0.5) from (−15.78±0.86) mV to (−11.24 ±0.77) mV (n=6, P<0.05) and the slope (K) from 5.33±0.74 to 4.35±0.74 (n=6, P>0.05). However, it did not alter the shapes of I–V curve, steady-state inactivation curve, or recovery from inactivation curve.

Conclusions

SAA inhibited I-CaL in a dose-dependent manner. It shifted the steady-state activation curve to a more positive voltage, which indicated that the drug affected the activated state of calcium channels, and suggested that the Ca2+ antagonistic effect of SAA be beneficial in the treatment of myocardial ischemia reperfusion injury.

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Authors and Affiliations

  1. The Laboratory Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China

    Bao Wang  (王 宝), Jian-xun Liu  (刘建勋), Hong-xu Meng  (孟红旭) & Cheng-ren Lin  (林成仁)

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  1. Bao Wang  (王 宝)
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  2. Jian-xun Liu  (刘建勋)
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  3. Hong-xu Meng  (孟红旭)
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  4. Cheng-ren Lin  (林成仁)
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Corresponding author

Correspondence to Jian-xun Liu  (刘建勋).

Additional information

Supported by the Key Project of National Science Foundation of China (No. 30830118) and the National Key New Drug Project (No. 2009ZX09301-005 and No. 2009ZX09303-003).

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Wang, B., Liu, Jx., Meng, Hx. et al. Blocking effect of salvianolic acid a on calcium channels in isolated rat ventricular myocytes. Chin. J. Integr. Med. 18, 366–370 (2012). https://doi.org/10.1007/s11655-011-0707-1

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  • DOI: https://doi.org/10.1007/s11655-011-0707-1

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