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Identification and quantitative determination of feruloyl-glucoside from hairy root cultures of Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb. (Cactaceae)

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Abstract

Transformed hairy root cultures have become an alternative for the biosynthesis of plant secondary metabolites with biological activities. In this present work, the effects of liquid Murashige and Skoog (MS) and Gamborg B5 (B5) medium on kinetic behavior, biomass and phenolic metabolite production were analyzed in Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb. hairy root cultures. Liquid MS medium showed the highest biomass production (13.67 g L−1 dry weight) after 77 d of culture. For B5 medium, highest biomass was achieved sooner, at day 56, but with lower total biomass (8.10 g L−1 dry weight). After isolation, structural elucidation of the major compound present in T. lophophoroides hairy roots was determined by nuclear magnetic resonance and mass spectral analysis. As a result, a ferulic acid derivative (feruloyl-glucoside) was isolated from T. lophophoroides hairy roots and reported for the first time. Quantitative analysis indicated that feruloyl-glucoside was the major phenolic metabolite at 56 d of growth in MS medium (2.7267 ± 0.041 mg g−1 dry weight L−1) and at 7 and 35 d in B5 medium (2.6328 ± 0.108 and 2.4372 ± 0.026 mg g−1 dry weight L−1, respectively). The feruloyl-glucoside was not detected in untransformed roots (control). The present results suggested the potential of T. lophophoroides hairy roots culture for the production of this phenolic glycoside.

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Funding

This work was supported by the Departamento de Apoyo a la Investigación de la Universidad Autónoma de Aguascalientes (UAA) (Grants PIBT16-16). G.J. Solis-Castañeda is indebted to Consejo Nacional de Ciencia y Tecnología (CONACyT-México) for the doctoral fellowship awarded. Y.A. Gómez-Aguirre is grateful to Cátedras-CONACyT.

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Authors and Affiliations

  1. Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, 20131, Aguascalientes, Aguascalientes, Mexico

    Gladis J Solis-Castañeda, Emmanuel Cabañas-García, Eugenio Pérez-Molphe-Balch & Yenny A Gómez-Aguirre

  2. Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, 62730, Xochitepec, Morelos, Mexico

    Alejandro Zamilpa

  3. Centro de Investigaciones Químicas-IICBA, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, CP, 62209, México

    Silvia Marquina Bahena

  4. CONACyT Research Fellow-Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ciudad Universitaria, Aguascalientes, Aguascalientes, 20131, México

    Yenny A Gómez-Aguirre

Authors
  1. Gladis J Solis-Castañeda
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  2. Alejandro Zamilpa
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  3. Emmanuel Cabañas-García
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  4. Silvia Marquina Bahena
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  5. Eugenio Pérez-Molphe-Balch
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  6. Yenny A Gómez-Aguirre
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Correspondence to Yenny A Gómez-Aguirre.

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Editor: Praveen Saxena

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Figure S1.

Full mass spectrometry scan data in negative ion mode of Peak 1 (m/z 355.1) of Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb. hairy roots cultures grown in liquid Murashige and Skoog (MS; Murashige and Skoog 1962) at 56 d (exponential growth phase) (PNG 111 kb)

High resolution image (EPS 14.2 MB)

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Solis-Castañeda, G.J., Zamilpa, A., Cabañas-García, E. et al. Identification and quantitative determination of feruloyl-glucoside from hairy root cultures of Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb. (Cactaceae). In Vitro Cell.Dev.Biol.-Plant 56, 8–17 (2020). https://doi.org/10.1007/s11627-019-10029-z

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  • DOI: https://doi.org/10.1007/s11627-019-10029-z

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