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Cryopreservation of red raspberry cultivars from the VIR in vitro collection using a modified droplet vitrification method

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Abstract

The goal of the present study was to analyze the post-cryogenic recovery of 12 red raspberry cultivars from the N.I. Vavilov All-Russian Institute of Plant Genetic Resources in vitro collection. The 1.1–1.8 mm shoot tips of microplants were subjected to cryopreservation using the modified droplet vitrification method. The current modifications to the droplet vitrification protocol included the elimination of the initial pretreatment stage of the microplants, and the use of modified media at the stages of initial micropropagation, explant isolation, and post-cryogenic regeneration. The optimized method reduced the duration of some cryopreservation stages compared to the initial protocol, and reduced the total procedure from 14 to 11 wk. This modified cryopreservation method also demonstrated a relatively high level of post-cryogenic regeneration. Depending on the genotype, the shoot recovery of explants after rewarming varied from 24.2–89.3% and averaged 58.8 ± 5.3%. There was a statistically significant influence of the genotype on the shoot recovery after rewarming. No differences in inter simple sequence repeats and in start codon targeted marker spectra were found between post-cryopreservation microplants and donor in vitro plants from two red raspberry cultivars.

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References

  • Anonymous (2015) Annual Report of the ICAR-National Bureau of Plant Genetic Resources 2014–2015, ICAR-NBPGR, Pusa Campus, New Delhi, India, 210+x p

  • Antonova OY, Dunaeva SE, Ukhatova YV, Kamylina NY, Dolganova NA, Lisicyna OV, Gavrilenko TA (2015) In vitro improvement of raspberry varieties from raspberry bushy dwarf virus (RBDV) using complex therapy method. Dostizheniya nauki i tekhniki APK 29(7):61–64 (in Russian)

    Google Scholar 

  • Bologovskaja RP (1949) Malina [Raspberry]. Sel’hozgiz Publ, Moscow-Leningrad

    Google Scholar 

  • Castillo NRF, Bassil NV, Wada S, Reed BM (2010) Genetic stability of cryopreserved shoot tips of Rubus germplasm. In Vitro Cell Dev Biol–Plant 46:246–256

  • Catalog of Raspberry cultivars. Available at: strawberryfarm.info/raspberry-sort-45.html. Cited 25 Jan 2017

  • Collard BCY, Mackill DJ (2009) Start Codon Targeted (SCoT) Polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep 27:86

    Article  CAS  Google Scholar 

  • Condello E, Ruzić D, Panis B, Caboni E (2011) Raspberry cryopreservation by droplet vitrification technique. Acta Hortic 918:965–969

    Article  Google Scholar 

  • Debnath SC (2004) Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. Plant Growth Reg 43:179–186

    Article  CAS  Google Scholar 

  • EU GEN RES “European small berries genetics resources” Available at: https://www.bordeaux.inra.fr/genberry/doc/dissemination/genres036/GENRES036-European-raspberry-germplasm-list.pdf Cited 25 Jan 2017

  • Gavrilenko T, Antonova O, Shuvalova A, Krylova E, Alpatyeva N, Spooner D, Novikova L (2013) Genetic diversity and origin of cultivated potatoes based on plastid microsatellite polymorphism. Genet Resour Crop Evol 60:1997–2015

    Article  CAS  Google Scholar 

  • Graham J, McNicol RJ, Greig K, Van de Ven WTG (1994) Identification of red raspberry cultivars and an assessment of their relatedness using fingerprints produced by random primers. J Hort Sci 69:123–130

    Google Scholar 

  • Gupta S, Reed BM (2006) Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification. Cryo-Letters 27:29–42

    PubMed  Google Scholar 

  • Kaczmarczyk A, Houben A, Keller ER, Mette MF (2010) Influence of cryopreservation on the cytosine methylation state of potato genomic DNA Cryo-Letters 31(5):380–391

  • Kaczmarczyk A, Rokka V-M, Keller ERJ (2011) Potato shoot tip cryopreservation. A review. Potato Res 54:45–79

    Article  Google Scholar 

  • Keller ERJ, Senula A, Leunufna S, Grűbe M (2006) Slow growth storage and cryopreservation—tools to facilitate germplasm maintenance of vegetatively propagated crops in living plant collections. Int J Refrig 29:411–417

    Article  Google Scholar 

  • Kovalchuk I, Turdiev T, Kushnarenko S, Rakhimbaev I, Reed BM (2010) Cryopreservation of raspberry cultivars: testing techniques for long-term storage of Kazakhstan’s plant germplasm In: Turuspekov Y (Ed) Khazakhstan Plant Science and Biotechnology.The Asian Aust J Plant Sci Biotech 4(Special Issue 1):1–4

  • Kovalchuk IY, Turdiev TT, Uspanova GK, Madiyeva GA, Chukanova NI, Reed BМ (2014) Cryopreservation and cold storage of fruit, berry crops and grape germplasm in Kazakhstan. In: Proceedings Plant biology and biotechnology international conference May 28-30, Almaty, Kazakhstan: P. 43

  • Lamoureux D, Sorokin A, Lefèvre I, Alexanian S (2011) Investigation of genetic diversity in Russian collections of raspberry and blue honeysuckle. Plant Genet Resour 9(2):202–205

    Article  CAS  Google Scholar 

  • Lefèvre I, Ziebel J, Guignard C, Sorokin A, Tikhonova O, Dolganova N, Hoffmann L, Eyzaguirre P, Hausman J-F (2011) Evaluation and comparison of nutritional quality and bioactive compounds of berry fruits from Lonicera caerulea. Ribes L species and Rubus idaeus grown in Russia J Berry Res 1:159–167

  • Matsumoto T (2017) Cryopreservation of plant genetic resources: conventional and new methods. Rev Agric Sci 5:13–20

    Google Scholar 

  • Matsumoto T, Sakai A, Yamada K (1994) Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia Japonica) by vitrification and subsequent high plant regeneration. Plant Cell Rep 13:442–446

    Article  CAS  PubMed  Google Scholar 

  • Mix-Wagner G, Schumacher HM, Cross RJ (2002) Recovery of potato apices after several years of storage in liquid nitrogen. Cryo-Letters 24:33–41

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nicuţă D, Rotilă G, Ciobanu Ş (2014) Aspects regarding the in vitro multiplication of the Rubus hirtus L. species. Studii şi Cercetări Biologie 23(1):79–84

    Google Scholar 

  • Nukari A, Uosukainen M (2007) Cryopreservation in the Finnish national germplasm programme for horticultural plants. Adv Hort Sci 21(4):232–234

  • Nukari A, Uosukainen M, Laamanen J, Rantala S (2011) Cryopreservation of horticultural plants at MTT. In: Grapin A, Keller ERJ, Lynch PT, Panis B, Revilla Bahillo A, Engelmann F (eds) Proceeding of the final meeting COST Action 871 CryoPlanet “Cryopreservation of crop species in Europe”. pp. 93-97

  • Nukari A, Uosukainen M, Rokka V-M, Antonius K, Wang Q, Valkonen JPT (2009) Cryopreservation techniques and their application in vegetatively propagated crops in Finland. Agric Food Sci 18:117–128

    Article  Google Scholar 

  • Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci 168:45–55

    Article  CAS  Google Scholar 

  • Panis B, Van den Houwe I, Swennen R, Rhee J, Roux N (2016) Securing plant genetic resources for perpetuity through cryopreservation. Indian J Plant Genet Resour 29(3):300–302

    Article  Google Scholar 

  • Reed BM (2008) Cryopreservation of temperate berry crops. In: Reed BM (ed) Plant cryopreservation: A practical guide. Springer, New York, pp 333–364

    Chapter  Google Scholar 

  • Reed BM, DeNoma J (2012) Tissue Culture and Cryopreservation. In: Hummer K (ed) Corvallis repository annual report for 2012. United States Department of Agriculture, https://iapreview.ars.usda.gov/SP2UserFiles/Place/20721500/AnnualReports/CorvallisAnnualReport2012.pdf, pp 29. Cited 05 Apr 2017

  • Reed BM, Engelmann F, Dulloo ME, Engels JMM (2004) Technical guidelines for the management of field and in vitro germplasm collections. Handbooks for Genebanks No. 7. Rome, IPGRI

  • Sakai A (1997) Potentially valuable cryogenic procedures for cryopreservation of cultured plant meristems. In: Razdan MK, Cocking EC (eds) Conservation of plant genetic resource in vitro. Science Publishers, USA, pp 53–66

    Google Scholar 

  • Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nuclear cells of navel orange (Citrus sinensis Osb. var.brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:3–33

    Article  Google Scholar 

  • Schäfer-Menuhr A, Schumacher HM, Mix-Wagner G (1997) Long-term storage of old potato varieties by cryopreservation of shoot-tips in liquid nitrogen. Plant Genet Resour Newsl 111:19–24

    Google Scholar 

  • Solov’eva AI, Vysotskaya ON, Dolgikh YI (2016) Effect of dehydration duration of apices on characteristics of in vitro plants of Fragaria vesca after cryopreservation. Russ J Plant Physiol 63(2):243–251

    Article  Google Scholar 

  • Towill LE (1983) Improved survival after cryogenic exposure of shoot tips derived from in vitro plantlet cultures of potato. Cryobiology 20:567–573

    Article  CAS  PubMed  Google Scholar 

  • Vysotskaya ON, Popov AS (2005) Method for cryogenic in vitro keeping of meristems isolated from red raspberry plants. Patent RF, no. 2248121 http://russianpatents.com/patent/224/2248121.html Cited 25 Jan 2017

  • Wang B, Wang RR, Cui ZH, Bi WL, Li JW, Li BQ, Ozudogru EA, Volk GM, Wang QC (2014) Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication. Biotechnol Adv 32:583–595

    Article  CAS  PubMed  Google Scholar 

  • Wang LY, Li YD, Sun HY, Liu HG, Tang XD, Wang QC, Zhang ZD (2017) An efficient droplet-vitrification cryopreservation for valuable blueberry germplasm. Sci Hortic 219:60–69

    Article  CAS  Google Scholar 

  • Wang QC, Laamanen J, Uosukainen M, Valkonen JPT (2005) Cryopreservation of in vitro grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Rep 24:280–288

    Article  PubMed  Google Scholar 

  • Zayova E, Stancheva I, Geneva M, Petrova M, Dimitrova L (2016) Comparison of antioxidant activity of the fruits derived from in vitro propagated and traditionally cultivated tayberry plants. J Sci Food Agric 96:3477–3483

    Article  CAS  PubMed  Google Scholar 

  • Zhang Z, Skjeseth G, Elameen A, Haugslien S, Sivertsen A, Clarke L, Wang Q-C, Blystad DR (2015) Field performance evaluation and genetic integrity assessment in Argyranthemum ‘Yellow Empire’ plants recovered from cryopreserved shoot tips. Vitro Cell Dev Biol– Plant 51(5):505–513

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Funding

The paper has been prepared with the support from the Program of Fundamental Research of the State Academies of Sciences (Russian Federation) for 2013–2020.

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Authors and Affiliations

  1. Department of Biotechnology, Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42-44, Bolshaya Morskaya Street, St. Petersburg, Russia, 190000

    Y. V. Ukhatova, S. E. Dunaeva, O. Y. Antonova, O. V. Apalikova, K. S. Pozdniakova, L. Y. Novikova, L. E. Shuvalova & T. A. Gavrilenko

  2. St. Petersburg State University, 7/9, Universitetskaya Embassy, St. Petersburg, Russia, 199034

    T. A. Gavrilenko

Authors
  1. Y. V. Ukhatova
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  2. S. E. Dunaeva
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  3. O. Y. Antonova
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  4. O. V. Apalikova
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  5. K. S. Pozdniakova
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  6. L. Y. Novikova
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  7. L. E. Shuvalova
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  8. T. A. Gavrilenko
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Corresponding author

Correspondence to T. A. Gavrilenko.

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Editor: Barbara Reed

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Ukhatova, Y.V., Dunaeva, S.E., Antonova, O.Y. et al. Cryopreservation of red raspberry cultivars from the VIR in vitro collection using a modified droplet vitrification method. In Vitro Cell.Dev.Biol.-Plant 53, 394–401 (2017). https://doi.org/10.1007/s11627-017-9860-3

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  • DOI: https://doi.org/10.1007/s11627-017-9860-3

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