Abstract
In vitro conservation of Mandevilla moricandiana was performed by slow-growth storage and encapsulation–dehydration. For slow-growth storage, half- and full-strength Murashige and Skoog (MS) medium and Woody Plant Medium, with or without sorbitol, mannitol, or glucose, were used to test the development of nodal segments and maintenance of plant viability after 6 mo. Recovery was performed using MS medium. The basal medium and carbon source did not interact, and only the carbon source had a significant effect on slow-growth storage and recovery. Sorbitol and glucose, individually or in combination, promoted development of plants with a low multiplication rate during the slow-growth period and a high multiplication rate during the recovery period. For encapsulation–dehydration, nonencapsulated and sodium alginate-encapsulated nodal segments were evaluated to determine their viability after storage at different temperatures. Nonencapsulated nodal segments gave 16.6% recovery after 60 d at 25°C. The effects of preculturing encapsulated nodal segments in MS medium with 0.4 or 0.75 M sucrose followed by dehydration were also tested. Capsules precultured for 48 h in the presence of 0.40 M sucrose and dehydrated to 40% moisture content showed 93.3% recovery. These conditions were then used to store capsules under different temperatures and for different lengths of time. The precultured capsules showed ca. 30% recovery after storage for 30 d at 4°C. Well-developed plantlets regenerated from encapsulated, stored nodal segments were rooted and acclimatized successfully, with 100% survival.
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Acknowledgments
The authors thank CAPES (Conselho de Administração de Pessoal de Ensino Superior) for a doctoral scholarship for the first author; PBV-UFRJ (Programa de Pós-graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro) and FAPERJ (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro) for their financial support; Prof. Dr. Tatiana Ungaretti Paleo Konno of UFRJ-Macaé for providing seeds of M. moricandiana; taxonomists Prof. Dr. Jorge Fontella Pereira of Museu Nacional (UFRJ), MSc. Marcelo Fraga Castilhiori, and Inaldo do Espírito Santo of Herbarium Bradeanum for species identification; Prof. Dr. Aline Vieira Santos (PBV-UFRJ) for valuable assistance with encapsulation–dehydration protocols; Universidade Federal do Estado do Rio de Janeiro (UNIRIO) for providing transport to the collection areas; the Brazilian Institute for Environment and Natural Renewable Resources (IBAMA) for authorization to collect (Scientific Research Activities no. 18498–1); and specially to Dr. Randall P. Niedz (US Horticultural Research Laboratory) and to the editor of this Journal, Dr. John J. Finer, for their valuable contributions and suggestions to improve this manuscript.
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Editor: Randy Niedz
This study is part of doctoral thesis of the first author
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Cordeiro, S.Z., Simas, N.K., Henriques, A.B. et al. In vitro conservation of Mandevilla moricandiana (Apocynaceae): short-term storage and encapsulation–dehydration of nodal segments. In Vitro Cell.Dev.Biol.-Plant 50, 326–336 (2014). https://doi.org/10.1007/s11627-014-9600-x
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DOI: https://doi.org/10.1007/s11627-014-9600-x