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In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments

  • Micropropagation
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Abstract

Acanthostachys strobilacea (Schult. f.) Klotzsch is an ornamental species of Bromeliaceae that may show an elongated stem when cultivated in vitro. This work reports a micropropagation protocol for A. strobilacea using nodal segments. Seeds were placed in Murashige and Skoog’s medium with macronutrients diluted to 1/5. Nodal segments isolated from the stems of in vitro elongated plants were subcultured in the same medium and kept in different light intensities (14, 41, and 50 μmol m−2 s−1) or continuous darkness. Another group of nodes was subcultured according to the position in the mother seedling. The plants that showed the most stem elongation were those that were cultured in 14 μmol m−2 s−1 or that came from isolated nodal segments in the median and basal regions of the mother plant. After 2 mo, all of the plants originating from the development of lateral buds were transferred to a greenhouse. Only those that were not elongated survived ex vitro and flowered after 1 yr.

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Acknowledgment

This work was supported by CAPES for the scholarship awarded to D.S.S.

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Authors and Affiliations

  1. Instituto de Botânica da Secretaria do Meio Ambiente do Estado de São Paulo, Núcleo de Pesquisa em Plantas Ornamentais (NPPO), Caixa Postal 4005, 01061-970, São Paulo, São Paulo, Brazil

    Daniela Soares dos Santos, Vívian Tamaki & Catarina Carvalho Nievola

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  1. Daniela Soares dos Santos
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  2. Vívian Tamaki
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  3. Catarina Carvalho Nievola
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Correspondence to Daniela Soares dos Santos.

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Editor: E. Bunn

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Santos, D.S., Tamaki, V. & Nievola, C.C. In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments. In Vitro Cell.Dev.Biol.-Plant 46, 524–529 (2010). https://doi.org/10.1007/s11627-010-9325-4

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  • DOI: https://doi.org/10.1007/s11627-010-9325-4

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